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Cic (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Cic
Gene Name:  capicua homolog (Drosophila)
Sequence             
Original Clone ID: FLH270372.01L
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23512657
Title: Structural basis of protein complex formation and reconfiguration by polyglutamine disease protein Ataxin-1 and Capicua
PMID: 23512657
Title: Structural basis of protein complex formation and reconfiguration by polyglutamine disease protein Ataxin-1 and Capicua
Authors: The ORFeome Collaboration
MmCD00083297

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4818nts         Open reading frame : 1 to 4818

ATGTACTCGGCCCACAGGCCCCTGATACCCGCGTCTGGCGCGGCCTCTCGTGGCCTCGGC
ATGTTCGTGTGGACAAATGTCGAACCTCGTTCTGTGGCTGTGTTCCCCTGGCACTCCTTA
GTCCCCTTCCTGGCACCCAGCCAGCCCGACCCCTCTGTGCAGCCTAGTGAGGCCCAGCAA
CCTGCCAGCCACCCTGTGGCCTCCAACCAGAGCAAAGAACCTGCTGAGTCTGCTGCTGTT
GCTCATGAGCAGCCACCAGGAGGGACGGGGGGTGCTGACCCTGGACGGCCCCCTGGAGCA
GTGTGCCCTGAGAGCCCAGGGCCTGGACCTCCACTTACTTTGGGTGGTGTGGATCCTGGT
AAAAGTCTCCCCCCCACCACTGAGGAGGAGGCTCCTGGCCCTCCAGGAGAGCCCCGGCTG
GACAGTGAGACCGAGAGTGATCATGATGATGCCTTCCTCTCCATCATGTCTCCTGAGATT
CAGTTGCCTCTGCCACCTGGAAAGCGCCGCACCCAGTCTCTGAGTGCCTTGCCCAAGGAA
CGAGACTCATCTTCTGAAAAGGATGGACGAAGTCCTAACAAGCGGGAGAAGGACCATATT
CGTCGGCCCATGAATGCCTTCATGATCTTCAGCAAGCGGCACCGGGCCTTGGTCCACCAG
CGGCACCCCAACCAGGACAACCGGACTGTCAGCAAGATCCTGGGCGAGTGGTGGTACGCC
CTGGGGCCCAAAGAGAAGCAGAAATACCACGACCTGGCCTTCCAGGTGAAAGAGGCCCAC
TTCAAGGCCCACCCAGATTGGAAATGGTGCAACAAGGACCGAAAGAAGTCCAGCTCAGAG
GCCAAGCCTGCGAGCCTGGGTCTGGCAGGAGGGCACAAGGAGACGCGGGAACGGAGCATG
TCGGAGACGGGAACTGCTGCTGCCCCTGGGGTGTCTTCTGAGCTCCTGTCTGTTGCTGCC
CAGACACTTTTGAGCTCGGACACCAAGGTTCCAGGGAGTGGCCCCTGTGGAGCAGAACGG
CTACACGCAGTTGGGGCACCTGGCTCAGCCCGACCCAGAGCCTTCTCCCACAGTGGGGTG
CACAGTCTTGATGGTGGGGAAGTAGACAGCCAAGCACTTCAAGAACTGACCCAGATGGTT
TCTGGCCCCGCATCATACTCCGGCCCAAAGCCTTCCCCCCAGTATGGTGCTCCAGGATCT
TTTGCAGCTCCTGGTGAAGGAGGTACCTTGGCCACTAGTGGGCGGCCTCCACTGTTGCCC
TCCCGAGCCTCTCGTTCCCAGCGTGCAGCCAGTGAGGACATGACCAGTGATGAGGAGCGG
ATGGTCATCTGTGAGGAAGAAGGGGATGATGATGTCATTGCTGATGACAGCTTTGGCACC
ACCGACATTGATCTCAAGTGCAAGGAACGAGTGACTGACAGTGAAAGTGGAGACAGCTCT
GGGGAGGACCCAGAGGGTAACAAGGGCTTTGGCCGTAAGGTGTTCTCACCTGTCATCCGC
TCCTCCTTTACCCATTGCCGTCCAACCCTGGACCCTGAGCCTCCAGGGCCCCCGGATCCA
CCTGCAGCCTTCAGCAAAGGCTACGGTCCCACCCCATCATCCTCCTCTTCACCTGCTTCC
ACCTCAGTCTCAGTCTCCACCTCCTTTTCACTGGGCTCTGGAACCTTTAAGACCCAGGAG
TCTGGTCAGGGCAGCACAGCGGTGCCACTACGGCCCCCACCCCCTGGAGCCGGGGGCCCA
GCAACACCTTCCAAGGCCACTCGGTTTCCTCCTACGGATTCTGCCACCTTTCGGCGCAAG
AGACCTGAAAGTGTTGGTAGCCTTGAAGCACCAGGCCCCTCTGTCATTGCAGCACCTCCC
AGTGGGGGAGGAAACCTTCTGCAGACACTGGTTCTGCCTCCGAGCAAGGAGGATCGGGAG
GGTACACGAGTGCCCTCAGCCCCAGCCCCACCACTGGCTTATGGGGCTCCCGCAGCCCCT
CTGTGCCGCCCTGCTGCCACCATGGTCACCAATGTGGTACGGCCTGTCAGCAGCACTCCT
GTGCCCATTGCCTCTAAGCCCTTTCCCACCTCTGGTCGGGCTGAGGCATCTTCAAATGAC
ATAGCAGGTGCCAGGACTGAAATGGGCACAGGATCCCGGGTGCCTGGGGGCTCCCCAATG
GGTGTCAGTTTAGTGTATTCAGATAAGAAGTCAGCAGCAGCAGCCACCTCGCCAGCTCCA
CATTTGGTAGCTGGACCTTTATTGGGCACTGTGGGGAAGGCACCTGCTACTGTCACCAAC
TTGCTGGTAGGCACCCCAGGCTATGGGGCTCCTGCATCGCCTGCTGTTCAGTTTATTGCC
CAGGGAGCCCCAGGCAGTGCAACCCCTGCAGGCTCAGGAGCAAGTACTGGGAGTGGCCCC
AATGGGCCAGTACCCCTGGGCATCCTGCAGCCAGGTGCCCTAGGCAAGGCTGGGGGAATC
ACACAGGTGCAGTACATCCTGCCCACACTGCCCCAGCAGCTTCAAGTGGCACCTGCCCCA
GCACCAGCCCCTGGGACCAAGGCAGCAGCTCCCAGTGGCCCTGCACCCACCACCAGCATC
CGTTTCACCCTCCCTCCGGGCACCTCGACCAACGGCAAGGTCCTGGCTGCCACTGCACCC
ACTGCTGGCATCCCTATCCTGCAGTCCGTACCCTCAGCCCCACCCCCTAAAGCCCAGTCA
GTTTCTCCTGTCCAGGCCACACCTTCAGGTGGCTCAGCCCAGCTGCTGCCTGGGAAGGTG
CTAGTCCCCCTGGCTGCCCCTAGCATGTCAGTTCGAGGTGGAGGGGCTGGTCAGCCACTG
CCCCTGGTTAGCTCGCCTTTCTCAGTACCTGTCCAAAATGGTGCCCAACAACCTAGCAAG
ATTATCCAGCTGACTCCTGTGCCTGTGAGCACACCTAGTGGCCTGGTACCACCCCTGAGC
CCAGCTACAATGCCGGGACCCACATCACAGCCTCAGAAGGTCCTGTTGCCCTCTTCCACA
AGAATCACCTATGTACAGTCAGCAGGTGGGCACACTCTGCCTCTAGGCACCAGTTCTGCA
TGCAGTCAGACTGGAACAGTGACCTCATATGGGCCCACCAGCTCTGTAGCTCTGGGCTTC
ACATCTTTGGGGCCCAGTGGTCCTGCCTTTGTACAGCCTCTGCTCTCAGCAGGCCAAGCT
CCTTTGCTGGCTCCTGGCCAGGTGGGCGTGTCACCTGTGCCTAGTCCCCAGTTGCCTCCT
GCCTGTACAGCCTCTGGAGGTCCTGTCATAACAGCATTTTACCCTGGCAGCCCTGCACCC
ACTTCAGCACCCCTGGGCCCACCTTCCCAAGCTCCGCCAAGCCTGGTCTACACTGTAGCC
ACCAGTACCACTCCACCTGCTGCTACCATTCTGCCCAAGGGCCCACCAGCCTCTGCCACT
GCCACTCCAGCCCCTACTAGTCCTTTCCCTAGCGCCACAGCAGGCTCCATGACCTACAGC
TTAGTGGCTCCCAAAGCTCAGCGACCCAGCCCAAAAGCTCCCCAGAAAGTAAAGGCAGCC
ATTGCCAGCATTCCTGTGGGGTCTTTTGAATCGGGTACTACTGGGCGGCCTGGATCTACA
CCCCGACAGTCTTCAGACTCTGGCGTAGCCAGAGAGCCTGCTGCCCCAGAATCAGAACTT
GAGGGGCAGCCCACACCCCCAGCTCCCCCACCCCCCACAGAGACCTGGCCTCCCACTGCC
CGGAGCAGTCCCCCACCCCCCTTGCCTGCTGAGGAGCGACCTGGCACTAAAGGCCCTGAG
ACTGCCAGCAAATTCCCCAGCTCATCTTCAGACTGGCGAGTTCCTGGGCTGGGCCTGGAG
AGTCGTGGGGAGCCTCCTACCCCTCCCAGCCCAGCTCCAGCCACAGGCCCCAGTGGAAGC
AGCAGTGGCAGCAGCGAGGGCAGTAGTGGGAGGGCAGCTGGGGACACACCCGAGCGCAAG
GAAGTCACTAGTTCTGGCAAGAAGATGAAGGTGCGGCCCCCACCCCTGAAGAAGACCTTT
GACTCTGTGGACAACAGGGTCCTGTCAGAAGTGGACTTTGAAGAGCGGTTTGCTGAGCTG
CCGGAGTTTAGACCAGAGGAGGTGCTGCCCTCACCCACCCTGCAGTCTCTGGCCACCTCG
CCTCGGGCTATCCTCGGCTCCTACCGAAAGAAGAGGAAGAATTCCACGGACCTAGACTCA
GCACCTGAAGATCCCACCTCACCCAAGCGCAAGATGAGGAGACGTTCGAGCTGCAGCTCA
GAGCCCAACACCCCCAAGAGTGCCAAGTGCGAGGGGGACATCTTCACCTTTGACCGCACA
GGTACTGAGACAGAGGATGTGCTCGGGGAGCTGGAGTATGAGAAGGTGCCCTACTCATCA
CTGCGGCGCACCCTGGACCAACGGCGGGCCCTGGTCATGCAGCTCTTCCAGGACCATGGC
TTCTTCCCATCAGCCCAGGCCACAGCAGCCTTCCAGGCCCGCTACGCAGACATCTTCCCA
TCCAAGGTGTGTCTGCAATTAAAGATCCGAGAGGTCCGCCAGAAGATCATGCAGGCAGCC
ACTCCCACAGAGCAGCCCCCTGGGGCTGAAGCCCCCCTCCCTGGACCACCCCCTACTGGC
ATGGCTGCTACTCCTGTCCCCACTCCCAGCCCTGCTGGGGGCCCTGACCCCACCTCTCCA
GGCTCGGACTCTGGCACTGCCCAAGTTGCCCCGCCACTGCCTCCACCCCCAGAGCCTGGG
CCTGGACAGCCTGGCTGGGAGGGGGCTCCCCAGCCCTCACCACCTCCCTCTGGCCCTTCC
ACAGCTGCCACAGGCAGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4861
Start on reference sequence 41
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Cic
Symbol Nomenclature : Cic
SYNONYM : 1200010B10Rik; mKIAA0306
Designation : protein capicua homolog
Full Nomenclature : capicua homolog (Drosophila)
GENEID : 71722
GI : 157169771
GenBank Accession : BC152804
Mouse Genome Informatics : 1918972
Vega : OTTMUSG00000036029
Target GenBank: NM_027882

 

Protein

SMART domain : HMG : high mobility group
UniProt : Q924A2

Clone

Cloning Information : 270372
HIP Master Clone ID : 201874
IMAGE ID : 100016068
Original Clone ID : FLH270372.01L

Organism


TAX_ID : 10090
Species Specific ID: 71722

Genome

Chromosome : 7
Map Location : 7 A3|7
Ensembl : ENSMUSG00000005442

Reagents

Labome : Cic-antibody