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NT5C2 (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NT5C2
Gene Name:  5'-nucleotidase, cytosolic II
Sequence             
Original Clone ID: 1025.1.36_GO.34926
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00083722

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1760nts         Open reading frame : 28 to 1734
Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCTCAACCTCCTGG
AGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTAACATGGATAAGCATGCCCTGAAA
AAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAG
ATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTAT
GAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAG
TTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCAGGGGACTTGTCTTTGACACACTG
TATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACCTCTTGGTCTGTGCACATGGATTT
AACTTTATAAGGGGACCAGAAACTAGAGAACAGTATCCAAATAAATTTATCCAGCGAGAT
GATACTGAAAGATTTTACATTCTGAACACACTATTCAACCTACCAGAGACCTACCTGTTG
GCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTT
AAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTT
GACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTAT
GTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTA
TTTCTTGCTACCAACAGTGACTATAAATATACAGATAAAATTATGACTTACCTGTTTGAC
TTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGACCATGGCAGTCCTACTTTGACTTG
ATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAGAAGGCACAGTACTGCGTCAGGTG
GATACTAAAACTGGCAAGCTGAAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATC
GTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGAC
ATTTTGTATATTGGAGATCACATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGG
TGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGGAGCTACATGTCTGGACTGACAAG
AGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTTTCTTGGCTGAACTCTACAAGCAT
CTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTTCCATCCAGAGACGTATTAAGAAA
GTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTTCGCAGTGGCTCC
CGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTC
ATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCT
CATGAATCAACGGTGGAGCACACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCC
ACCCGGAACCGCACATCAGTGGATTTCAAAGACACTGACTACAAGCGGCACCAGCTGACA
CGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCCCACTGGCCCCCCAGGAAATTACA
CACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGAGGAGGAAGAATAATAGGACCCA
GCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1707
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NT5C2
Symbol Nomenclature : NT5C2
SYNONYM : GMP; NT5B; PNT5; cN-II
Designation : 5'-nucleotidase (purine), cytosolic type B|IMP-specific 5'-NT|cytosolic 5'-nucleotidase II|cytosolic purine 5'-nucleotidase|purine 5' nucleotidase
Full Nomenclature : 5'-nucleotidase, cytosolic II
GENEID : 22978
Locus Tag : RP11-30H12.3
HGNC : 8022
MIM : 600417
Vega : OTTHUMG00000018981
Target GenBank: BC001595

 

Reference Sequence Alignment

  

HsCD00083722        ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCTCAACCTCCTGG
NM_001134373.2      ---------------------------------------------ATGTCAACCTCCTGG
NM_012229.4         ---------------------------------------------ATGTCAACCTCCTGG
                                                                 :  ************

HsCD00083722        AGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTAACATGGATAAGCATGCCCTGAAA
NM_001134373.2      AGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTAACATGGATAAGCATGCCCTGAAA
NM_012229.4         AGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTAACATGGATAAGCATGCCCTGAAA
                    ************************************************************

HsCD00083722        AAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAG
NM_001134373.2      AAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAG
NM_012229.4         AAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAG
                    ************************************************************

HsCD00083722        ATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTAT
NM_001134373.2      ATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTAT
NM_012229.4         ATAAAGTGTTTTGGTTTTGATATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTAT
                    ************************************************************

HsCD00083722        GAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAG
NM_001134373.2      GAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAG
NM_012229.4         GAGTCCCTTGGTTTTGAGCTTACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAG
                    ************************************************************

HsCD00083722        TTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCAGGGGACTTGTCTTTGACACACTG
NM_001134373.2      TTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCAGGGGACTTGTCTTTGACACACTG
NM_012229.4         TTGCTCAGCTTTGCTTATGATTCTACATTCCCTACCAGGGGACTTGTCTTTGACACACTG
                    ************************************************************

HsCD00083722        TATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACCTCTTGGTCTGTGCACATGGATTT
NM_001134373.2      TATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACCTCTTGGTCTGTGCACATGGATTT
NM_012229.4         TATGGAAATCTTTTGAAAGTCGATGCCTATGGAAACCTCTTGGTCTGTGCACATGGATTT
                    ************************************************************

HsCD00083722        AACTTTATAAGGGGACCAGAAACTAGAGAACAGTATCCAAATAAATTTATCCAGCGAGAT
NM_001134373.2      AACTTTATAAGGGGACCAGAAACTAGAGAACAGTATCCAAATAAATTTATCCAGCGAGAT
NM_012229.4         AACTTTATAAGGGGACCAGAAACTAGAGAACAGTATCCAAATAAATTTATCCAGCGAGAT
                    ************************************************************

HsCD00083722        GATACTGAAAGATTTTACATTCTGAACACACTATTCAACCTACCAGAGACCTACCTGTTG
NM_001134373.2      GATACTGAAAGATTTTACATTCTGAACACACTATTCAACCTACCAGAGACCTACCTGTTG
NM_012229.4         GATACTGAAAGATTTTACATTCTGAACACACTATTCAACCTACCAGAGACCTACCTGTTG
                    ************************************************************

HsCD00083722        GCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTT
NM_001134373.2      GCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTT
NM_012229.4         GCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTT
                    ************************************************************

HsCD00083722        AAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTT
NM_001134373.2      AAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTT
NM_012229.4         AAAGATGGGGACCTCTTCATGTCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTT
                    ************************************************************

HsCD00083722        GACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTAT
NM_001134373.2      GACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTAT
NM_012229.4         GACTGGGTTCATTACAAGGGCTCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTAT
                    ************************************************************

HsCD00083722        GTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTA
NM_001134373.2      GTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTA
NM_012229.4         GTAGTCAAAGATGGAAAACTGCCTTTGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTA
                    ************************************************************

HsCD00083722        TTTCTTGCTACCAACAGTGACTATAAATATACAGATAAAATTATGACTTACCTGTTTGAC
NM_001134373.2      TTTCTTGCTACCAACAGTGACTATAAATATACAGATAAAATTATGACTTACCTGTTTGAC
NM_012229.4         TTTCTTGCTACCAACAGTGACTATAAATATACAGATAAAATTATGACTTACCTGTTTGAC
                    ************************************************************

HsCD00083722        TTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGACCATGGCAGTCCTACTTTGACTTG
NM_001134373.2      TTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGACCATGGCAGTCCTACTTTGACTTG
NM_012229.4         TTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGACCATGGCAGTCCTACTTTGACTTG
                    ************************************************************

HsCD00083722        ATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAGAAGGCACAGTACTGCGTCAGGTG
NM_001134373.2      ATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAGAAGGCACAGTACTGCGTCAGGTG
NM_012229.4         ATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAGAAGGCACAGTACTGCGTCAGGTG
                    ************************************************************

HsCD00083722        GATACTAAAACTGGCAAGCTGAAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATC
NM_001134373.2      GATACTAAAACTGGCAAGCTGAAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATC
NM_012229.4         GATACTAAAACTGGCAAGCTGAAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATC
                    ************************************************************

HsCD00083722        GTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGAC
NM_001134373.2      GTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGAC
NM_012229.4         GTCTACTCAGGAGGTTCTTCTGATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGAC
                    ************************************************************

HsCD00083722        ATTTTGTATATTGGAGATCACATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGG
NM_001134373.2      ATTTTGTATATTGGAGATCACATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGG
NM_012229.4         ATTTTGTATATTGGAGATCACATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGG
                    ************************************************************

HsCD00083722        TGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGGAGCTACATGTCTGGACTGACAAG
NM_001134373.2      TGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGGAGCTACATGTCTGGACTGACAAG
NM_012229.4         TGGCGAACTTTTTTGGTGATTCCTGAACTCGCACAGGAGCTACATGTCTGGACTGACAAG
                    ************************************************************

HsCD00083722        AGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTTTCTTGGCTGAACTCTACAAGCAT
NM_001134373.2      AGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTTTCTTGGCTGAACTCTACAAGCAT
NM_012229.4         AGTTCACTTTTCGAAGAACTTCAGAGCTTGGATATTTTCTTGGCTGAACTCTACAAGCAT
                    ************************************************************

HsCD00083722        CTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTTCCATCCAGAGACGTATTAAGAAA
NM_001134373.2      CTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTTCCATCCAGAGACGTATTAAGAAA
NM_012229.4         CTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTTCCATCCAGAGACGTATTAAGAAA
                    ************************************************************

HsCD00083722        GTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTTCGCAGTGGCTCC
NM_001134373.2      GTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTTCGCAGTGGCTCC
NM_012229.4         GTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTTCGCAGTGGCTCC
                    ************************************************************

HsCD00083722        CGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTC
NM_001134373.2      CGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTC
NM_012229.4         CGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTC
                    ************************************************************

HsCD00083722        ATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCT
NM_001134373.2      ATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCT
NM_012229.4         ATCAACCTGCTGTATTACCCTTTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCT
                    ************************************************************

HsCD00083722        CATGAATCAACGGTGGAGCACACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCC
NM_001134373.2      CATGAATCAACGGTGGAGCACACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCC
NM_012229.4         CATGAATCAACGGTGGAGCACACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCC
                    ************************************************************

HsCD00083722        ACCCGGAACCGCACATCAGTGGATTTCAAAGACACTGACTACAAGCGGCACCAGCTGACA
NM_001134373.2      ACCCGGAACCGCACATCAGTGGATTTCAAAGACACTGACTACAAGCGGCACCAGCTGACA
NM_012229.4         ACCCGGAACCGCACATCAGTGGATTTCAAAGACACTGACTACAAGCGGCACCAGCTGACA
                    ************************************************************

HsCD00083722        CGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCCCACTGGCCCCCCAGGAAATTACA
NM_001134373.2      CGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCCCACTGGCCCCCCAGGAAATTACA
NM_012229.4         CGGTCAATTAGTGAGATTAAACCTCCCAACCTCTTCCCACTGGCCCCCCAGGAAATTACA
                    ************************************************************

HsCD00083722        CACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGAGGAGGAAGAATAATAGGACCCA
NM_001134373.2      CACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGAGGAGGAAGAATAA---------
NM_012229.4         CACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGAGGAGGAAGAATAA---------
                    ***************************************************         

HsCD00083722        GCTTTCTTGTACAAAGTGGT
NM_001134373.2      --------------------
NM_012229.4         --------------------
                                        

Pathways

Reactome : Abacavir metabolism
Reactome : Abacavir transport and metabolism
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Purine catabolism
Reactome : Purine metabolism

Protein

Structural Annotation Wiki : TOPSAN
UniProt : A8K6K2
UniProt : P49902
HPRD : 02686

Clone

Cloning Information : 268855
HIP Master Clone ID : 200430
Original Clone ID : 1025.1.36_GO.34926

Experimental

TargetTrack - Experimental data : GO.34926

Organism


TAX_ID : 9606
Species Specific ID: 22978

Genome

Chromosome : 10
Map Location : 10q24.32
Ensembl : ENSG00000076685

Reagents

Labome : NT5C2-antibody