Gene:
Gene Symbol:
TM0070
Gene Name:
endo-1,4-beta-xylanase B
Original Clone ID:
7031
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Next amino acid codon after CDS represents STOP, hence clone should be labeled for CDS 'fusion', overall 'closed'
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00083955
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1044nts
Open reading frame: 37 to 1080
ATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAAATATTACCTTCTGTGTTG
ATCCTTTTGTTGGGATGTGTTCCAGTTTTCAGCTCTCAGAATGTATCTCTGAGAGAACTC
GCAGAAAAGCTGAACATCTATATTGGTTTTGCCGCAATCAACAACTTTTGGTCTCTTTCC
GACGCAGAAAAGTACATGGAAGTTGCAAGAAGAGAATTCAACATCCTGACCCCTGAGAAC
CAGATGAAGTGGGATACGATTCATCCAGAAAGAGACAGATACAATTTCACTCCCGCTGAA
AAACACGTTGAGTTTGCAGAAGAAAACGACATGATCGTGCATGGACACACTCTTGTCTGG
CACAACCAGCTTCCTGGATGGATCACTGGTAGAGAATGGACAAAGGAAGAACTTTTGAAC
GTTCTTGAAGACCACATAAAAACGGTGGTGTCTCATTTCAAAGGTAGAGTGAAGATCTGG
GATGTGGTGAACGAAGCGGTGAGCGATTCTGGAACCTACAGGGAAAGCGTGTGGTACAAG
ACGATCGGTCCTGAATACATTGAAAAAGCGTTCAGATGGGCAAAAGAAGCCGATCCAGAT
GCGATTCTCATCTACAACGACTACAGCATAGAAGAAATCAACGCAAAATCGAACTTCGTC
TACAACATGATAAAAGAGCTGAAAGAAAAGGGAGTACCTGTTGATGGAATAGGATTTCAG
ATGCACATAGACTACAGAGGGCTCAATTATGACAGTTTCAGAAGGAATTTGGAGAGATTT
GCGAAACTCGGTCTTCAAATATACATCACAGAGATGGATGTGAGAATTCCTCTCAGTGGT
TCGGAGGAGTATTATTTGAAAAAACAGGCTGAAGTTTGTGCGAAGATCTTCGATATATGC
TTGGACAACCCTGCAGTTAAAGCGATCCAGTTTTGGGGATTCACAGACAAATACTCCTGG
GTTCCCGGCTTTTTCAAAGGGTACGGGAAAGCGTTGCTCTTCGATGAGAATTACAACCCC
AAGCCTTGTTATTACGCGATAAAAGAGGTGCTGGAGAAAAAGATAGAAGAAAGAAAATAA
GTTTAAACGGTCTCCAG
Protein sequence predicted based on nucleotide sequence
MKILPSVLILLLGCVPVFSSQNVSLRELAEKLNIYIGFAAINNFWSLSDAEKYMEVARRE FNILTPENQMKWDTIHPERDRYNFTPAEKHVEFAEENDMIVHGHTLVWHNQLPGWITGRE WTKEELLNVLEDHIKTVVSHFKGRVKIWDVVNEAVSDSGTYRESVWYKTIGPEYIEKAFR WAKEADPDAILIYNDYSIEEINAKSNFVYNMIKELKEKGVPVDGIGFQMHIDYRGLNYDS FRRNLERFAKLGLQIYITEMDVRIPLSGSEEYYLKKQAEVCAKIFDICLDNPAVKAIQFW GFTDKYSWVPGFFKGYGKALLFDENYNPKPCYYAIKEVLEKKIEERK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1044
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
