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FLJ44620 (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ADAL
Gene Name:  adenosine deaminase-like
Sequence             
Original Clone ID: 1306.1.5_GO.57322
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00084118

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 825nts         Open reading frame : 28 to 852
Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCATAGAGGCAGAA
GAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAAGTGGAACTTCATGCC
CACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATAGCCCAGAAGCCAGAT
CTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAAAGAACTTTGGAAGAA
TGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCTGAAGATATTCTAATG
GTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAGTACCTGGAACTAAGG
AGCACACCCAGAAGAGAAAATGCTACCGGAATGACTAAAAAGACTTATGTGGAATCTATA
CTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGATGTTAGGTATTTGATA
GCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTAAAACTTGCCGAGGAG
TTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGTGGAGACCCTACTGTA
GGACAAGCAAAAGACTTCTTGGAACCTCTTTTAGAAGCTAAGAAAGCAGGTCTGAAGTTA
GCATTGCATCTTTCAGAGATTCCAAACCAAAAAAAAGAAACACAAATACTCCTGGATCTG
CTTCCTGACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGAGGGAGGATCCCTGGAT
CTGGTGGACTTTGTGAGGCAACATCGGATACCACTGGGTAAGGCTTGGAGTTTCAGGTCT
TCCAGATGATAGGACCCAGCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 825
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ADAL
Symbol Nomenclature : ADAL
Designation : adenosine deaminase-like protein
Full Nomenclature : adenosine deaminase-like
GENEID : 161823
HGNC : 31853
Vega : OTTHUMG00000176646
Target GenBank: BC075857

 

Reference Sequence Alignment

  

HsCD00084118        ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCATAGAGGCAGAA
NM_001012969.3      ---------------------------------------------ATGATAGAGGCAGAA
                                                                 :  ************

HsCD00084118        GAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAAGTGGAACTTCATGCC
NM_001012969.3      GAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAAGTGGAACTTCATGCC
                    ************************************************************

HsCD00084118        CACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATAGCCCAGAAGCCAGAT
NM_001012969.3      CACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATAGCCCAGAAGCCAGAT
                    ************************************************************

HsCD00084118        CTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAAAGAACTTTGGAAGAA
NM_001012969.3      CTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAAAGAACTTTGGAAGAA
                    ************************************************************

HsCD00084118        TGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCTGAAGATATTCTAATG
NM_001012969.3      TGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCTGAAGATATTCTAATG
                    ************************************************************

HsCD00084118        GTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAGTACCTGGAACTAAGG
NM_001012969.3      GTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAGTACCTGGAACTAAGG
                    ************************************************************

HsCD00084118        AGCACACCCAGAAGAGAAAATGCTACCGGAATGACTAAAAAGACTTATGTGGAATCTATA
NM_001012969.3      AGCACACCCAGAAGAGAAAATGCTACTGGAATGACTAAAAAGACTTATGTGGAATCTATA
                    ************************** *********************************

HsCD00084118        CTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGATGTTAGGTATTTGATA
NM_001012969.3      CTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGATGTTAGGTATTTGATA
                    ************************************************************

HsCD00084118        GCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTAAAACTTGCCGAGGAG
NM_001012969.3      GCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTAAAACTTGCCGAGGAG
                    ************************************************************

HsCD00084118        TTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGTGGAGACCCTACTGTA
NM_001012969.3      TTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGTGGAGACCCTACTGTA
                    ************************************************************

HsCD00084118        GGACAAGCAAAAGACTTCTTGGAACCTCTTTTAGAAGCTAAGAAAGCAGGTCTGAAGTTA
NM_001012969.3      GGACAAGCAAAAGACTTCTTGGAACCTCTTTTAGAAGCTAAGAAAGCAGGTCTGAAGTTA
                    ************************************************************

HsCD00084118        GCATTGCATCTTTCAGAGATTCCAAACCAAAAAAAAGAAACACAAATACTCCTGGATCTG
NM_001012969.3      GCATTGCATCTTTCAGAGATTCCAAACCAAAAAAAAGAAACACAAATACTCCTGGATCTG
                    ************************************************************

HsCD00084118        CTTCCTGACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGAGGGAGGATCCCTGGAT
NM_001012969.3      CTTCCTGACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGAGGGAGGATCCCTGGAT
                    ************************************************************

HsCD00084118        CTGGTGGACTTTGTGAGGCAACATCGGATACCACTGGGTAAGGCTTGGAGTTTCAGGTCT
NM_001012969.3      CTGGTGGACTTTGTGAGGCAACATCGGATACCACTGGGTAAGGCTTGGAGTTTCAGGTCT
                    ************************************************************

HsCD00084118        TCCAGATGATAGGACCCAGCTTTCTTGTACAAAGTGGT
NM_001012969.3      TCCAGATGA-----------------------------
                    *********                             

Pathways

Reactome : Abacavir metabolism
Reactome : Abacavir transport and metabolism
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Purine metabolism
Reactome : Purine salvage

Protein

Structural Annotation Wiki : TOPSAN
UniProt : Q6DHV7
UniProt : B4DQM8
HPRD : 11779

Clone

Cloning Information : 273247
HIP Master Clone ID : 203115
Original Clone ID : 1306.1.5_GO.57322

Experimental

TargetTrack - Experimental data : GO.57322

Organism


TAX_ID : 9606
Species Specific ID: 161823

Genome

Chromosome : 15
Map Location : 15q15.3
Ensembl : ENSG00000168803

Reagents

Labome : ADAL-antibody