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ADSL (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ADSL
Gene Name:  adenylosuccinate lyase
Sequence             
Original Clone ID: 1265.1.32_GO.37383
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00084176

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1476nts         Open reading frame : 28 to 1503
Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGGCTGGAGGC
GATCATGGTTCGCCCGACAGCTACCGCTCACCTCTTGCCTCCCGCTATGCCAGCCCGGAG
ATGTGCTTCGTGTTTAGCGACAGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGG
CTGGCGGAGGCCGAGCAGACATTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATG
AAATCAAACCTGGAGAACATCGACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGA
CATGATGTGATGGCTCACGTGCACACATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATT
ATTCACCTTGGTGCTACTTCTTGCTATGTTGGAGACAATACTGACTTGATTATTCTTAGA
AATGCACTTGACCTGCTTTTGCCAAAGCTTGCCAGAGTGATCTCTCGGCTTGCCGACTTT
GCTAAGGAACGAGCCAGTCTACCCACATTAGGTTTCACACATTTCCAGCCTGCACAGCTG
ACCACAGTTGGGAAACGTTGCTGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAAC
TTGAAGCGTGTCCGAGATGACCTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAG
GCCAGTTTCCTGCAGCTCTTTGAGGGAGATGACCATAAGGTAGAGCAGCTTGACAAGATG
GTGACAGAAAAGGCAGGATTTAAGAGAGCTTTCATCATCACAGGGCAGACATATACACGA
AAAGTGGATATTGAAGTACTGTCTGTGCTGGCTAGCTTGGGGGCATCAGTGCACAAGATT
TGCACCGACATACGCCTCCTGGCAAACCTCAAGGAGATGGAGGAACCCTTTGAAAAACAG
CAGATTGGCTCAAGTGCGATGCCATATAAGCGGAATCCCATGCGTTCAGAACGTTGCTGC
AGTCTTGCCCGCCACCTGATGACCCTTGTCATGGACCCGCTACAGACAGCATCTGTCCAG
TGGTTTGAACGCACACTGGATGATAGTGCCAACCGACGGATCTGTTTGGCCGAGGCATTT
CTTACCGCAGATACTATATTGAATACGCTGCAGAACATTTCTGAAGGATTGGTCGTGTAC
CCCAAAGTAATTGAACGGCGCATTCGGCAAGAGCTGCCTTTCATGGCCACAGAGAACATC
ATCATGGCCATGGTCAAAGCTGGAGGTAGCCGCCAGGATTGCCATGAGAAAATCAGAGTG
CTTTCTCAGCAGGCAGCTTCTGTGGTTAAGCAGGAAGGGGGTGACAATGACCTCATAGAG
CGTATCCAGGTTGATGCCTACTTCAGTCCCATTCACTCCCAGTTGGATCATTTACTGGAT
CCTTCTTCTTTCACTGGTCGTGCCTCCCAGCAGGTGCAGAGATTCTTAGAAGAGGAGGTG
TATCCCCTGTTAAAACCATATGAAAGCGTGATGAAGGTGAAAGCAGAATTATGTCTGTAG
TAGGACCCAGCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1476
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ADSL
Symbol Nomenclature : ADSL
SYNONYM : AMPS; ASASE; ASL
Designation : adenylosuccinase
Full Nomenclature : adenylosuccinate lyase
GENEID : 158
Locus Tag : RP5-1042K10.8
HGNC : 291
MIM : 608222
Target GenBank: BC000253

 

Reference Sequence Alignment

  

HsCD00084176      ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGGCTGGAGGC
NM_000026.2       ---------------------------------------------ATGGCGGCTGGAGGC
                                                               :  ************

HsCD00084176      GATCATGGTTCGCCCGACAGCTACCGCTCACCTCTTGCCTCCCGCTATGCCAGCCCGGAG
NM_000026.2       GATCATGGTTCGCCCGACAGCTACCGCTCACCTCTTGCCTCCCGCTATGCCAGCCCGGAG
                  ************************************************************

HsCD00084176      ATGTGCTTCGTGTTTAGCGACAGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGG
NM_000026.2       ATGTGCTTCGTGTTTAGCGACAGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGG
                  ************************************************************

HsCD00084176      CTGGCGGAGGCCGAGCAGACATTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATG
NM_000026.2       CTGGCGGAGGCCGAGCAGACATTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATG
                  ************************************************************

HsCD00084176      AAATCAAACCTGGAGAACATCGACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGA
NM_000026.2       AAATCAAACCTGGAGAACATCGACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGA
                  ************************************************************

HsCD00084176      CATGATGTGATGGCTCACGTGCACACATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATT
NM_000026.2       CATGATGTGATGGCTCACGTGCACACATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATT
                  ************************************************************

HsCD00084176      ATTCACCTTGGTGCTACTTCTTGCTATGTTGGAGACAATACTGACTTGATTATTCTTAGA
NM_000026.2       ATTCACCTTGGTGCTACTTCTTGCTATGTTGGAGACAATACTGACTTGATTATTCTTAGA
                  ************************************************************

HsCD00084176      AATGCACTTGACCTGCTTTTGCCAAAGCTTGCCAGAGTGATCTCTCGGCTTGCCGACTTT
NM_000026.2       AATGCACTTGACCTGCTTTTGCCAAAGCTTGCCAGAGTGATCTCTCGGCTTGCCGACTTT
                  ************************************************************

HsCD00084176      GCTAAGGAACGAGCCAGTCTACCCACATTAGGTTTCACACATTTCCAGCCTGCACAGCTG
NM_000026.2       GCTAAGGAACGAGCCAGTCTACCCACATTAGGTTTCACACATTTCCAGCCTGCACAGCTG
                  ************************************************************

HsCD00084176      ACCACAGTTGGGAAACGTTGCTGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAAC
NM_000026.2       ACCACAGTTGGGAAACGTTGCTGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAAC
                  ************************************************************

HsCD00084176      TTGAAGCGTGTCCGAGATGACCTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAG
NM_000026.2       TTGAAGCGTGTCCGAGATGACCTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAG
                  ************************************************************

HsCD00084176      GCCAGTTTCCTGCAGCTCTTTGAGGGAGATGACCATAAGGTAGAGCAGCTTGACAAGATG
NM_000026.2       GCCAGTTTCCTGCAGCTCTTTGAGGGAGATGACCATAAGGTAGAGCAGCTTGACAAGATG
                  ************************************************************

HsCD00084176      GTGACAGAAAAGGCAGGATTTAAGAGAGCTTTCATCATCACAGGGCAGACATATACACGA
NM_000026.2       GTGACAGAAAAGGCAGGATTTAAGAGAGCTTTCATCATCACAGGGCAGACATATACACGA
                  ************************************************************

HsCD00084176      AAAGTGGATATTGAAGTACTGTCTGTGCTGGCTAGCTTGGGGGCATCAGTGCACAAGATT
NM_000026.2       AAAGTGGATATTGAAGTACTGTCTGTGCTGGCTAGCTTGGGGGCATCAGTGCACAAGATT
                  ************************************************************

HsCD00084176      TGCACCGACATACGCCTCCTGGCAAACCTCAAGGAGATGGAGGAACCCTTTGAAAAACAG
NM_000026.2       TGCACCGACATACGCCTCCTGGCAAACCTCAAGGAGATGGAGGAACCCTTTGAAAAACAG
                  ************************************************************

HsCD00084176      CAGATTGGCTCAAGTGCGATGCCATATAAGCGGAATCCCATGCGTTCAGAACGTTGCTGC
NM_000026.2       CAGATTGGCTCAAGTGCGATGCCATATAAGCGGAATCCCATGCGTTCAGAACGTTGCTGC
                  ************************************************************

HsCD00084176      AGTCTTGCCCGCCACCTGATGACCCTTGTCATGGACCCGCTACAGACAGCATCTGTCCAG
NM_000026.2       AGTCTTGCCCGCCACCTGATGACCCTTGTCATGGACCCGCTACAGACAGCATCTGTCCAG
                  ************************************************************

HsCD00084176      TGGTTTGAACGCACACTGGATGATAGTGCCAACCGACGGATCTGTTTGGCCGAGGCATTT
NM_000026.2       TGGTTTGAACGCACACTGGATGATAGTGCCAACCGACGGATCTGTTTGGCCGAGGCATTT
                  ************************************************************

HsCD00084176      CTTACCGCAGATACTATATTGAATACGCTGCAGAACATTTCTGAAGGATTGGTCGTGTAC
NM_000026.2       CTTACCGCAGATACTATATTGAATACGCTGCAGAACATTTCTGAAGGATTGGTCGTGTAC
                  ************************************************************

HsCD00084176      CCCAAAGTAATTGAACGGCGCATTCGGCAAGAGCTGCCTTTCATGGCCACAGAGAACATC
NM_000026.2       CCCAAAGTAATTGAACGGCGCATTCGGCAAGAGCTGCCTTTCATGGCCACAGAGAACATC
                  ************************************************************

HsCD00084176      ATCATGGCCATGGTCAAAGCTGGAGGTAGCCGCCAGGATTGCCATGAGAAAATCAGAGTG
NM_000026.2       ATCATGGCCATGGTCAAAGCTGGAGGTAGCCGCCAGGATTGCCATGAGAAAATCAGAGTG
                  ************************************************************

HsCD00084176      CTTTCTCAGCAGGCAGCTTCTGTGGTTAAGCAGGAAGGGGGTGACAATGACCTCATAGAG
NM_000026.2       CTTTCTCAGCAGGCAGCTTCTGTGGTTAAGCAGGAAGGGGGTGACAATGACCTCATAGAG
                  ************************************************************

HsCD00084176      CGTATCCAGGTTGATGCCTACTTCAGTCCCATTCACTCCCAGTTGGATCATTTACTGGAT
NM_000026.2       CGTATCCAGGTTGATGCCTACTTCAGTCCCATTCACTCCCAGTTGGATCATTTACTGGAT
                  ************************************************************

HsCD00084176      CCTTCTTCTTTCACTGGTCGTGCCTCCCAGCAGGTGCAGAGATTCTTAGAAGAGGAGGTG
NM_000026.2       CCTTCTTCTTTCACTGGTCGTGCCTCCCAGCAGGTGCAGAGATTCTTAGAAGAGGAGGTG
                  ************************************************************

HsCD00084176      TATCCCCTGTTAAAACCATATGAAAGCGTGATGAAGGTGAAAGCAGAATTATGTCTGTAG
NM_000026.2       TATCCCCTGTTAAAACCATATGAAAGCGTGATGAAGGTGAAAGCAGAATTATGTCTGTAG
                  ************************************************************

HsCD00084176      TAGGACCCAGCTTTCTTGTACAAAGTGGT
NM_000026.2       -----------------------------
                                               

Pathways

Panther : De novo purine biosynthesis
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Purine metabolism
Reactome : Purine ribonucleoside monophosphate biosynthesis
WikiPathway : Nucleotide Metabolism

Protein

SMART domain : ADSL_C : Adenylosuccinate lyase C-terminus
Structural Annotation Wiki : TOPSAN
UniProt : P30566
HPRD : 00049

Clone

Cloning Information : 273325
HIP Master Clone ID : 203193
Original Clone ID : 1265.1.32_GO.37383

Experimental

TargetTrack - Experimental data : GO.37383

Organism


TAX_ID : 9606
Species Specific ID: 158

Genome

Chromosome : 22
Map Location : 22q13.2
Ensembl : ENSG00000239900