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PEPD (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PEPD
Gene Name:  peptidase D
Sequence             
Original Clone ID: 1265.1.33_GO.38734
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00084182

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1503nts         Open reading frame : 28 to 1530
Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGGCGGCCACC
GGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTG
AACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATC
GTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTC
CGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTC
ATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCC
ACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGAC
GTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTC
ACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGC
ATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTG
TTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCC
CACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTC
TTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGC
GGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGAGCTCCCAACGACCGA
ACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAGTATTACTGCTTCGCT
TCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGGCCGTC
TATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGG
TGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGC
ATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCT
CACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGAGGCTACCCAGAGGGC
GTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCACGGCACCTGCAGCCA
GGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCACCTCCTGGATGAGGCC
CTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTCGCGGT
TTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTG
ACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCC
TTTACCCCCTTCTCTGGCCCCAAGTAGTAGGACCCAGCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1503
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PEPD
Symbol Nomenclature : PEPD
SYNONYM : PROLIDASE
Designation : X-Pro dipeptidase|aminoacyl-L-proline hydrolase|imidodipeptidase|proline dipeptidase|xaa-Pro dipeptidase
Full Nomenclature : peptidase D
GENEID : 5184
HGNC : 8840
MIM : 613230
Vega : OTTHUMG00000180470
Target GenBank: BC004305

 

Reference Sequence Alignment

  

HsCD00084182      ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGGCGGCCACC
NM_000285.3       ---------------------------------------------ATGGCGGCGGCCACC
                                                               :  ************

HsCD00084182      GGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTG
NM_000285.3       GGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTG
                  ************************************************************

HsCD00084182      AACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATC
NM_000285.3       AACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATC
                  ************************************************************

HsCD00084182      GTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTC
NM_000285.3       GTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTC
                  ************************************************************

HsCD00084182      CGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTC
NM_000285.3       CGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTC
                  ************************************************************

HsCD00084182      ATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCC
NM_000285.3       ATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCC
                  ************************************************************

HsCD00084182      ACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGAC
NM_000285.3       ACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGAC
                  ************************************************************

HsCD00084182      GTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTC
NM_000285.3       GTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTC
                  ************************************************************

HsCD00084182      ACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGC
NM_000285.3       ACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGC
                  ************************************************************

HsCD00084182      ATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTG
NM_000285.3       ATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTG
                  ************************************************************

HsCD00084182      TTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCC
NM_000285.3       TTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCC
                  ************************************************************

HsCD00084182      CACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTC
NM_000285.3       CACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTC
                  ************************************************************

HsCD00084182      TTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGC
NM_000285.3       TTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGC
                  ************************************************************

HsCD00084182      GGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGAGCTCCCAACGACCGA
NM_000285.3       GGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGAGCTCCCAACGACCGA
                  ************************************************************

HsCD00084182      ACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAGTATTACTGCTTCGCT
NM_000285.3       ACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAGTATTACTGCTTCGCT
                  ************************************************************

HsCD00084182      TCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGGCCGTC
NM_000285.3       TCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGGCCGTC
                  ************************************************************

HsCD00084182      TATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGG
NM_000285.3       TATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGG
                  ************************************************************

HsCD00084182      TGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGC
NM_000285.3       TGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGC
                  ************************************************************

HsCD00084182      ATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCT
NM_000285.3       ATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCT
                  ************************************************************

HsCD00084182      CACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGAGGCTACCCAGAGGGC
NM_000285.3       CACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGAGGCTACCCAGAGGGC
                  ************************************************************

HsCD00084182      GTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCACGGCACCTGCAGCCA
NM_000285.3       GTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCACGGCACCTGCAGCCA
                  ************************************************************

HsCD00084182      GGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCACCTCCTGGATGAGGCC
NM_000285.3       GGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCACCTCCTGGATGAGGCC
                  ************************************************************

HsCD00084182      CTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTCGCGGT
NM_000285.3       CTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTCGCGGT
                  ************************************************************

HsCD00084182      TTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTG
NM_000285.3       TTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTG
                  ************************************************************

HsCD00084182      ACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCC
NM_000285.3       ACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCC
                  ************************************************************

HsCD00084182      TTTACCCCCTTCTCTGGCCCCAAGTAGTAGGACCCAGCTTTCTTGTACAAAGTGGT
NM_000285.3       TTTACCCCCTTCTCTGGCCCCAAGTAG-----------------------------
                  ***************************                             

Protein

SMART domain : AMP_N : Aminopeptidase P, N-terminal domain
Structural Annotation Wiki : TOPSAN
UniProt : P12955
UniProt : E9PCE8
UniProt : A8MX47
UniProt : B4DDB7
HPRD : 01358

Clone

Cloning Information : 273333
HIP Master Clone ID : 203201
Original Clone ID : 1265.1.33_GO.38734

Experimental

TargetTrack - Experimental data : GO.38734

Organism


TAX_ID : 9606
Species Specific ID: 5184

Genome

Chromosome : 19
Map Location : 19q13.11
Ensembl : ENSG00000124299

Reagents

Labome : PEPD-antibody