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zgc:101717 (Danio rerio) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  cradd
Gene Name:  CASP2 and RIPK1 domain containing adaptor with death domain
Sequence             
Original Clone ID: 1322.1.2_GO.74129
Keyword: None
Species: Danio rerio
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
DrCD00084205

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 624nts         Open reading frame : 28 to 651

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGACCCAAAGCAC
AAAAAACTGCTCCGAACACAGCGGCTTTTTCTGTCCGAGGAGCTTTTAGTGGACGATTTA
ATCATTCAGTATTTATATCAAGAGGAGATACTGACGGAGAGCCATTTAGAGGAGATTCAG
TCCGAGTCGTCAAGCAGAAATAAAACGTTAAAGCTGCTGGATATTTTACCGAGCCGCGGA
GCGACAGCCTTCCATCACTTCATAAAGTCACTGGAGAAAGACTTTCCCTGGATCAGAGAC
AAACTGCTGCAACTAAACGAGGAAGAAGAGTCTCCTCCTCCGCCCTTCACAGTTCAGTGT
GGTGTTCCTGACCACATCCTTCTGACCGTACCAACCACCCTGCATCTGAATCAGCTGGCA
TCCCGTCTGGGCTCAGAGTGGAAGTCTGTGCTGCTGGATCTGGGCCTGTCCTGTGCGGAT
CTGTACCGGTGCTGCTCTGACCACCCGCTTGCTGTTCAGAGTCAGGTGCTCGCTGGACTG
GTGATGTGGACGCAGAGGAACGGAAGAGGGGCCACGGTGCAGCGGCTGATCCAGAGCCTG
CAGGCTGCAGATGTCCCGCCATCTGTGCTCCAACAGGTGTTTAAGTGATAGGACCCAGCT
TTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 624
Mutations:
None
Discrepancy:
g510a,G170G;
                                

5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : cradd
Symbol Nomenclature : cradd
SYNONYM : zgc:101717
Designation : death domain-containing protein CRADD
Full Nomenclature : CASP2 and RIPK1 domain containing adaptor with death domain
GENEID : 450046
Locus Tag : DKEYP-90A8.3
Zebrafish Database : ZDB-GENE-041010-168
Target GenBank: BC083256

 

Clone

Cloning Information : 273417
HIP Master Clone ID : 203285
Original Clone ID : 1322.1.2_GO.74129

Experimental

TargetTrack - Experimental data : GO.74129

Organism


TAX_ID : 7955
Species Specific ID: 450046

Genome

Chromosome : 4

Reagents

Labome : cradd-cDNA