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Detailed Vector Information: pMCSG10

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG10              
Source: M. Donnelly
Description: with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial;
Comments: None
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084258

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG10

pMCSG10 in advanced viewed

Synonyms: pMCSG-10
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial;
Comments: pMCSG7 derivative
Size (bp): 5961
Parent Vector: None
Properties: bacterial expression, improves solubility of insert, multiple cloning site, with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation independent cloning (LIC) site 204 235
bacterial origin ori ColE1-type origin of replication 3955 3955
promoter T7 T7 promoter sequence 1035 1051
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene lacI lacI coding sequence 1565 2524
selectable marker AmpR ampicillin resistance gene 4713 5573
tag His N-terminal 6xHis tag 943 960
tag His 2 6xHis tag 140 157
tag GST GST tag 265 924
trxn termination sequence T7 term T7 terminator sequence 26 72

 

Other Link: SBKB Technology Portal
Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None