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Detailed Vector Information: pMCSG11

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG11              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084259

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  50ug/mL chloramphenicol
Growth Condition:  Growth in LB/chloramphenicol (50ug/mL) at 37 degrees C
Comments:  None
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Vector Name: pMCSG11

pMCSG11 in advanced viewed

Synonyms: pMCSG-11
Sequencing Primer: Forward:  DuetUp1
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4079
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS2 MCS (BamHI, EcoRI, HindIII, AvaI) 2171 2216
MCS MCS MCS (NcoI, BamHI, EcoRI, PstI, HindIII) 1801 1880
MCS LIC ligation-independent cloning (LIC) region 353 384
bacterial origin ori P15A-type bacterial origin of replication 1821 2733
primer site Duet Down1 Duet DOWN1 primer site 189 208
primer site Duet P1 ACYC Duet U P1 primer site 4020 4039
primer site Duet P2 Duet P2 primter site 189 208
promoter T7 T7 promoter 214 230
promoter T7 2 T7 promoter 4063 4079
protease cleavage site TEV TEV protease cleavage site 345 365
repressor binding site lacO2 lacO sequence region 235 230
repressor binding site lacO1 lacO sequence region 5 25
repressor protein gene lacI lacI cds 2857 3939
ribosome binding site RBS2 ribosome binding site 286 291
ribosome binding site RBS1 ribosome binding site 58 63
selectable marker CmR chloramphenicol resistance gene (CAT) 800 1459
tag His N-terminal His tag 83 100
tag His 2 6xHis tag 303 320
tag S-tag N-terminal S-tag 437 481
trxn termination sequence T7 term T7 termination sequence 533 580

 

Other Link: SBKB Technology Portal
Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None