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Detailed Vector Information: pMCSG12

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG12              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084260

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AVAILABLE
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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  50ug/mL chloramphenicol
Growth Condition:  Growth in LB/chloramphenicol (50ug/mL) at 37 degrees C
Comments:  None
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Vector Name: pMCSG12

pMCSG12 in advanced viewed

Synonyms: pMCSG-12
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4133
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 407 438
bacterial origin ori P15A-type bacterial origin of replication 1875 2787
gene fragment CAT CAT coding sequence 854 1513
promoter T7 T7 promoter 214 230
promoter T7 T7 promoter (upstream of another one) 4117 4133
protease cleavage site TEV TEV cleavage site cds 399 419
repressor protein gene LacI lacI coding sequence 2911 3993
ribosome binding site RBS RBS-1: ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
tag S loop N-terminal S loop 339 392
tag His N-terminal 6xHis tag 83 100
tag His 2 6xHis tag 303 320
trxn regulatory element lac operator lac operator sequence 5 25
trxn termination sequence T7 term T7 terminator sequence 587 634

 

Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None