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Detailed Vector Information: pMCSG14

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG14              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: None
Publications: None
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084262

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  50ug/mL chloramphenicol
Growth Condition:  Growth in LB/chloramphenicol (50ug/mL) at 37 degrees C
Comments:  None
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Vector Name: pMCSG14

pMCSG14 in advanced viewed

Synonyms: pMCSG-14
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4754
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 1028 1059
bacterial origin ori P15A-type bacterial origin of replication 2496 3408
gene fragment CAT CAT coding sequence 1475 2134
promoter T7 T7 promoter 214 230
protease cleavage site TEV TEV cleavage site cds 1020 1040
repressor binding site lac operator LacO fragment 5 25
repressor protein gene LacI LacI coding sequence 3532 4614
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
tag S-tag S-tag 1112 1156
tag His 6xHis tag 83 100
tag His 2 6xHis tag 978 995
tag GST Glutathione transferase (GST) tag 339 998
trxn termination sequence T7 term T7 terminator sequence 1208 1255

 

Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None