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Detailed Vector Information: pMCSG17

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG17              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084263

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pMCSG17

pMCSG17 in advanced viewed

Synonyms: pMCSG-17
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative
Size (bp): 5316
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 3291 3973
promoter T7 T7 promoter 390 406
protease cleavage site TEV TEV site cds 223 243
repressor binding site lac operator LacO fragment 365 387
repressor protein gene LacI LacI coding sequence 920 1879
selectable marker AmpR ampicillin resistance gene 4068 4928
tag His C-terminal 6xHis tag 140 157
tag S-tag N-terminal S-tag 271 315
trxn termination sequence T7 term T7 terminator 26 72

 

Other Link: SBKB Technology Portal
Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None