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Detailed Vector Information: pMCSG18

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG18              
Source: M. Donnelly
Description: Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC).
Comments: None
Publications: None
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084264

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AVAILABLE
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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pMCSG18

pMCSG18 in advanced viewed

Synonyms: pMCSG-18
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative
Size (bp): 6009
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 927 958
bacterial origin ori ColE1-type bacterial origin of replication 3984 4666
promoter T7 T7 promoter 1083 1099
protease cleavage site TEV TEV cleavage site cds 946 966
repressor binding site lac operator LacO fragment 1058 1080
repressor protein gene LacI LacI coding sequence 1613 2572
selectable marker AmpR ampicillin resistance gene 4761 5621
tag GFP GFP tag 208 927
tag His N-terminal 6xHis tag 991 1008
tag His 2 C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None