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Detailed Vector Information: pMCSG22

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG22              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084268

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
       DyNA Vector Map

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Vector Name: pMCSG22

pMCSG22 in advanced viewed

Synonyms: pMCSG-22
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 3906
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 407 438
bacterial origin ori CloDF-type bacterial origin of replication 1736 2474
promoter T7 2 T7 promoter 3890 3906
promoter T7 T7 promoter 3890 3906
promoter T7 T7 promoter (2) 214 230
protease cleavage site TEV TEV cleavage site cds 399 419
repressor binding site lac operator 2 LacO fragment 235 255
repressor binding site lac operator LacO fragment 5 25
repressor protein gene LacI LacI coding sequence 2684 3643
ribosome binding site RBS 3 Ribosome binding site 1602 1606
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
selectable marker SpectR Spectinomycin resistance gene (aadA) 805 1596
tag His 6xHis tag 83 100
tag His 2 6xHis tag 303 320
tag S loop S loop 339 392
trxn termination sequence T7 term T7 terminator sequence 587 634

 

Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None