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Detailed Vector Information: pMCSG24

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG24              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
EvNO00084270

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
       DyNA Vector Map

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Vector Name: pMCSG24

pMCSG24 in advanced viewed

Synonyms: pMCSG-24
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4527
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 1028 1059
bacterial origin ori CloDF-type origin of replication 2357 3095
promoter T7 T7 promoter 4511 4527
promoter T7 2 T7 promoter 214 230
protease cleavage site TEV TEV cleavage site cds 1020 1040
repressor binding site LacI LacI coding sequence 3305 4264
repressor binding site lac operator LacO fragment 5 25
repressor binding site lac operator 2 LacO fragment 235 255
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
ribosome binding site RBS 3 Ribosome binding site 2223 2227
selectable marker SpectR Spectinomycin resistance gene 1426 2217
tag His 6xHis tag 83 100
tag His 2 6xHis tag 303 320
tag GST GST tag 339 998
trxn termination sequence T7 term T7 terminator sequence 1208 1255

 

Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None