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Detailed Vector Information: pMCSG24

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG24              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084270

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AVAILABLE
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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
       DyNA Vector Map

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Vector Name: pMCSG24

pMCSG24 in advanced viewed

Synonyms: pMCSG-24
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4527
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 1028 1059
bacterial origin ori CloDF-type origin of replication 2357 3095
promoter T7 2 T7 promoter 214 230
promoter T7 T7 promoter 4511 4527
protease cleavage site TEV TEV cleavage site cds 1020 1040
repressor binding site lac operator 2 LacO fragment 235 255
repressor binding site lac operator LacO fragment 5 25
repressor binding site LacI LacI coding sequence 3305 4264
ribosome binding site RBS 3 Ribosome binding site 2223 2227
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
selectable marker SpectR Spectinomycin resistance gene 1426 2217
tag His 6xHis tag 83 100
tag His 2 6xHis tag 303 320
tag GST GST tag 339 998
trxn termination sequence T7 term T7 terminator sequence 1208 1255

 

Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None