Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pMCSG7
Source:
M. Donnelly
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
None
Publications:
PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
PMID: 23424251
Title: Modifying the catalytic preference of tributyrin in Bacillus thermocatenulatus lipase through in-silico modeling of enzyme-substrate complex
PMID: 23424251
Title: Modifying the catalytic preference of tributyrin in Bacillus thermocatenulatus lipase through in-silico modeling of enzyme-substrate complex
PMID: 24510647
Title: High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli.
PMID: 26573720
Title: Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5
PMID: 27074770
Title: Predicting the impact of mutations on the specific activity of Bacillus thermocatenulatus lipase using a combined approach of docking and molecular dynamics
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
PMID: 23424251
Title: Modifying the catalytic preference of tributyrin in Bacillus thermocatenulatus lipase through in-silico modeling of enzyme-substrate complex
PMID: 23424251
Title: Modifying the catalytic preference of tributyrin in Bacillus thermocatenulatus lipase through in-silico modeling of enzyme-substrate complex
PMID: 24510647
Title: High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli.
PMID: 26573720
Title: Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5
PMID: 27074770
Title: Predicting the impact of mutations on the specific activity of Bacillus thermocatenulatus lipase using a combined approach of docking and molecular dynamics
Authors:
M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
EvNO00084271
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
