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Detailed Vector Information: pMCentr2

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCentr2              
Source: M. Donnelly
Description: cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
Comments: None
Publications: None
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084276

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

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Vector Name: pMCentr2

pMCentr2 in advanced viewed

Synonyms: pMCentr-2
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: cloning vector to link to Gateway system with attL1-TEV-LIC site-attL2; kanamycin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
Comments: From pDONR221; LIC generates ENTR vector
Size (bp): 2586
Parent Vector: None
Properties: Gateway, donor (entry), fluorescent marker, ligation independent cloning (LIC), mammalian expression, recombinational cloning, ssDNA production, with tag/fusion/marker
Author Name: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 0 0
ligation independent cloning LIC ligation independent cloning (LIC) 679 710
primer site M13 forward sequencing primer site 537 552
primer site M13 reverse M13 reverse sequencing primer 851 867
protease cleavage site TEV protease cleavage site 671 691
recombination site attL2 attL2 recombination site 711 810
recombination site attL1 attL1 recombination site 569 668
selectable marker KanR kanamycin resistance gene 980 1789
trxn termination sequence rrnB rrnB T1 and T2 transcription terminator 268 470

 

Species Specific ID: None
Target GenBank: None