Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pEU-His-FV
Source:
CESG
Description:
Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments:
None
Publications:
PMID: 15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
PMID: 23662776
Title: Structural and functional characterization of CalS11, the TDPrhamnose 3¿-O-methyltransferase involved in calicheamicin biosynthesis
PMID: 18765284
Title: Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.
PMID: 19892197
Title: Cell-free translation of integral membrane proteins into unilamelar liposomes.
PMID: 20637905
Title: Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins.
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
PMID: 23662776
Title: Structural and functional characterization of CalS11, the TDPrhamnose 3¿-O-methyltransferase involved in calicheamicin biosynthesis
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
PMID: 23662776
Title: Structural and functional characterization of CalS11, the TDPrhamnose 3¿-O-methyltransferase involved in calicheamicin biosynthesis
PMID: 18765284
Title: Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.
PMID: 19892197
Title: Cell-free translation of integral membrane proteins into unilamelar liposomes.
PMID: 20637905
Title: Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins.
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
PMID: 23662776
Title: Structural and functional characterization of CalS11, the TDPrhamnose 3¿-O-methyltransferase involved in calicheamicin biosynthesis
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
EvNO00084283
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI,Promega
Special MTA: PSI,Promega
Recommended Growth Condition:
Distributed in bacterial strain:
BR610
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pEU-His-FV
Synonyms:
''
Sequencing Primer:
Forward:
pEU319 Forward
Reverse:
pEU Reverse
Description:
Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte. To avoid, the toxicity of the barnase gene, the vector pEU-HSBC (EvNO00306667) can be ordered, which substitutes sacB for barnase.
Size (bp):
4966
Parent Vector:
None
Properties:
cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
2809
3491
gene fragment
Omega
TMV Omega
397
520
gene fragment
TMV-L
TMV-L
278
2238
gene fragment
BarCat-bar
BarCat cassette, including Barnase
572
908
gene fragment
BarCat-cat
BarCat cassette, including CAT
1010
1667
primer
His2 forward
His2 forward sequencing primer TCTTTCAAATACTTCTAGCTAGAGTA
424
449
promoter
SP6
SP6 promoter
373
390
recombination site
MCS
pF1K (Flexi-Vector) homology domain
1675
1803
selectable marker
AmpR
ampicillin resistance gene
3589
4248
tag
His
6xHis tag
527
544
