Gene: |
Gene Symbol:
No insert Gene Name:  No insert |
---|---|
Original Clone ID: | None |
Type: | No insert |
Vector Name: | pEU-GST-FV |
Source: | CESG |
Description: | Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. |
Comments: | None |
Publications: |
PMID: 15782178 Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics PMID: 21899277 Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family. |
Authors: |
PSI Center for Eukaryotic Structural Genomics University of Wisconsin |
EvNO00084284 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI,Promega |
Distributed in bacterial strain : | BR610 |
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Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
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Vector Name: | pEU-GST-FV |
pEU-GST-FV in advanced viewed |
Synonyms: | None | |
Sequencing Primer: | Forward:
GST Forward
Reverse: pEU Reverse |
|
Description: | Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI). | |
Comments: | Cloning into this vector will remove the BarCat casstte | |
Size (bp): | 5612 | |
Parent Vector: | None | |
Properties: | cell-free expression, multiple cloning site, with tag/fusion/marker | |
Author Name: |
Center for Eukaryotic Structural Genomics |
|
Publications: |
PMID:
15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics |
|
Vector Map: |
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Sequence: |
Vector Features:
Type | Name | Description | Start Position | End Position |
---|---|---|---|---|
bacterial origin | ColE | ColE1 origin | 3081 | 3763 |
gene fragment | CAT | BarCat cassette, including CAT | 1301 | 1958 |
gene fragment | Omega | Omega | 25 | 148 |
gene fragment | Barnase | BarCat cassette, including Barnase | 863 | 1199 |
gene fragment | tet operator | TMV-L | 2466 | 2526 |
promoter | SP6 | SP6 promoter | 1 | 18 |
protease cleavage site | 3C | 3C cleavage site cds | 812 | 835 |
recombination site | MCS | pF1K (Flexi-Vector) homology domain | 1966 | 2094 |
selectable marker | AmpR | ampicillin resistance gene | 3861 | 4520 |
tag | GST | GST coding sequence (GST cds) | 149 | 805 |
trxn termination sequence | T7 term | T7 terminator sequence | 2075 | 2094 |
Species Specific ID: | None |
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Target GenBank: | None |