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Detailed Vector Information: pEU-GST-FV

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pEU-GST-FV              
Source: CESG
Description: Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments: None
Publications: PMID: 15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
PMID: 21899277
Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
Authors: PSI
Center for Eukaryotic Structural Genomics
University of Wisconsin
EvNO00084284

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AVAILABLE
No restriction
Special MTA: PSI,Promega

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : BR610
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pEU-GST-FV

pEU-GST-FV in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  GST Forward
Reverse:  pEU Reverse
Description: Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 5612
Parent Vector: None
Properties: cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3081 3763
gene fragment CAT BarCat cassette, including CAT 1301 1958
gene fragment Omega Omega 25 148
gene fragment Barnase BarCat cassette, including Barnase 863 1199
gene fragment tet operator TMV-L 2466 2526
promoter SP6 SP6 promoter 1 18
protease cleavage site 3C 3C cleavage site cds 812 835
recombination site MCS pF1K (Flexi-Vector) homology domain 1966 2094
selectable marker AmpR ampicillin resistance gene 3861 4520
tag GST GST coding sequence (GST cds) 149 805
trxn termination sequence T7 term T7 terminator sequence 2075 2094

 

Species Specific ID: None
Target GenBank: None