| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pEU-GST-FV |
| Source: | CESG |
| Description: | Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. |
| Comments: | None |
| Publications: |
PMID: 15782178 Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics PMID: 21899277 Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family. |
| Authors: |
PSI Center for Eukaryotic Structural Genomics University of Wisconsin |
|
EvNO00084284 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI,Promega |
| Distributed in bacterial strain : | BR610 |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
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| Vector Name: | pEU-GST-FV |
pEU-GST-FV in advanced viewed |
| Synonyms: | None | |
| Sequencing Primer: | Forward:
GST Forward
Reverse: pEU Reverse |
|
| Description: | Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI). | |
| Comments: | Cloning into this vector will remove the BarCat casstte | |
| Size (bp): | 5612 | |
| Parent Vector: | None | |
| Properties: | cell-free expression, multiple cloning site, with tag/fusion/marker | |
| Author Name: |
Center for Eukaryotic Structural Genomics |
|
| Publications: |
PMID:
15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | ColE | ColE1 origin | 3081 | 3763 |
| gene fragment | CAT | BarCat cassette, including CAT | 1301 | 1958 |
| gene fragment | Omega | Omega | 25 | 148 |
| gene fragment | Barnase | BarCat cassette, including Barnase | 863 | 1199 |
| gene fragment | tet operator | TMV-L | 2466 | 2526 |
| promoter | SP6 | SP6 promoter | 1 | 18 |
| protease cleavage site | 3C | 3C cleavage site cds | 812 | 835 |
| recombination site | MCS | pF1K (Flexi-Vector) homology domain | 1966 | 2094 |
| selectable marker | AmpR | ampicillin resistance gene | 3861 | 4520 |
| tag | GST | GST coding sequence (GST cds) | 149 | 805 |
| trxn termination sequence | T7 term | T7 terminator sequence | 2075 | 2094 |
| Species Specific ID: | None |
|---|---|
| Target GenBank: | None |
