PASD1 (Homo sapiens) in pJFT7_nHalo_DC(r4)

Explanation of Terms

Gene:
Gene Symbol:  PASD1
Gene Name:  PAS domain containing repressor 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00842864
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Insert sequence: 2319nts
Open reading frame: 1 to 2319

ATGAAGATGAGAGGGGAAAAGAGAAGAGACAAGGTCAATCCAAAAAGCTCTCAAAGGAAA
TTAAACTGGATTCCATCATTTCCTACCTATGATTACTTCAACCAAGTGACGCTACAGTTA
TTAGATGGCTTTATGATTACACTGAGCACAGATGGAGTGATCATTTGTGTGGCTGAAAAC
ATCTCTTCTCTTCTTGGACATTTACCAGCTGAGATTGTGGGCAAAAAATTATTAAGCCTT
CTGCCTGATGAAGAGAAAGATGAAGTCTACCAAAAGATTATTCTCAAATTTCCTTTACTA
AACTCAGAAACACATATTGAATTTTGCTGTCATTTAAAAAGAGGAAATGTCGAACATGGT
GATAGTTCTGCTTACGAAAACGTGAAATTTATTGTGAATGTAAGAGATATTTGTAATGAG
TTTCCTGTGGTCTTTAGTGGCTTGTTTTCCAGCCACCTCTGTGCTGACTTTGCTGCATGT
GTTCCTCAGGAGGATCGGCTTTATCTTGTGGGAAATGTTTGCATTCTCAGGACTCAGCTC
CTGCAGCAACTTTACACTTCAAAGGCAGTCAGTGATGAAGCTGTACTTACACAAGATTCA
GATGAGGAACCTTTTGTGGGAGAGCTCAGTAGCTCTCAAGGTCAAAGAGGACACACTAGC
ATGAAAGCCGTGTACGTTGAACCCGCTGCTGCTGCTGCTGCTGCTGCTATCTCAGACGAC
CAAATTGATATTGCAGAGGTTGAGCAGTATGGACCACAAGAAAACGTTCACATGTTTGTA
GATTCTGATTCAACTTATTGCTCCAGTACAGTTTTCCTGGATACTATGCCTGAATCTCCA
GCCTTATCCTTGCAAGACTTTCGAGGTGAGCCTGAGGTGAATCCATTGTACAGGGCAGAC
CCAGTGGACCTGGAGTTCTCGGTGGATCAGGTGGACTCAGTGGACCAGGAGGGCCCAATG
GACCAGCAGGACCCAGAGAACCCAGTTGCCCCGTTGGACCAGGCAGGCCTGATGGATCCA
GTGGATCCAGAGGACTCAGTGGACCTGGGGGCTGCTGGCGCAAGTGCTCAGCCATTACAG
CCATCATCACCAGTTGCATATGACATCATTAGCCAGGAACTGGAACTGATGAAGAAGTTG
AAGGAGCAGCTAGAAGAGAGGACTTGGTTGCTGCATGATGCCATCCAAAACCAGCAGAAT
GCATTGGAATTGATGATGGATCACCTTCAGAAGCAGCCAAACACATTACGCCACGTTGTC
ATTCCTGATCTCCAATCTTCGGAGGCAGTGCCCAAGAAACAACAGAAACAACACGCTGGG
CAAGTGAAGCGGCCTCTCCCACATCCCAAGGACGTCAAGTGTTTCTGTGGTTTATCTTTA
TCCAACTCTCTCAAAAACACTGGGGAGCTTCAGGAGCCTTGTGTTGCCTTCAACCAGCAG
CAACTGGTGCAGCAAGAACAACACCTGAAGGAGCAGCAGCGGCAGCTGCGGGAGCAGCTG
CAACAGCTGAGAGAGCAAAGGAAGGTGCAGAAGCAGAAGAAGATGCAGGAGAAGAAGAAG
CTGCAGGAGCAGAAAATGCAGGAGAAGAAGAAGCTGCAGGAGCAGAGGCGGCAAAAGAAG
AAGAAGCTACAGGAGCGGAAGAAGTGGCAGGGGCAGATGCTACAGAAAGAGCCAGAGGAG
GAGCAGCAGAAGCAGCAGCTGCAAGAGCAGCCACTGAAGCATAATGTCATCGTGGGGAAT
GAGAGGGTGCAGATATGCCTGCAAAACCCACGTGACGTATCTGTGCCCCTCTGCAATCAC
CCTGTTAGATTTTTACAGGCCCAACCCATTGTTCCTGTCCAGAGAGCAGCTGAACAACAG
CCCTCTGGCTTCTATCAAGATGAAAACTGTGGGCAACAGGAAGATGAGAGTCAAAGTTTT
TATCCTGAGGCGTATCAAGGGCCCCCCGTGAACCAGCTGCCATTGATAGATACCTCAAAC
TCTGAGGCAATTTCTTCTTCCAGCATTCCTCAGTTTCCCATAACTTCAGACTCAACCATA
AGCACCCTGGAGACCCCACAGGATTACATCCGGCTTTGGCAAGAGTTGTCTGATTCACTC
GGTCCTGTTGTCCAAGTGAACACTTGGTCTTGCGATGAGCAGGGCACCCTGCACGGCCAA
CCCACCTACCATCAGGTGCAAGTTTCTGAGGTAGGAGTCGAGGGACCTCCTGATCCACAG
GCTTTCCAAGGCCCTGCTGCATACCAGCCAGACCAGATGAGATCTGCGGAGCAGACCAGA
TTGATGCCTGCAGAGCAACGTGACTCAAATAAGCCGTGC

Protein sequence predicted based on nucleotide sequence

MKMRGEKRRDKVNPKSSQRKLNWIPSFPTYDYFNQVTLQLLDGFMITLSTDGVIICVAEN
ISSLLGHLPAEIVGKKLLSLLPDEEKDEVYQKIILKFPLLNSETHIEFCCHLKRGNVEHG
DSSAYENVKFIVNVRDICNEFPVVFSGLFSSHLCADFAACVPQEDRLYLVGNVCILRTQL
LQQLYTSKAVSDEAVLTQDSDEEPFVGELSSSQGQRGHTSMKAVYVEPAAAAAAAAISDD
QIDIAEVEQYGPQENVHMFVDSDSTYCSSTVFLDTMPESPALSLQDFRGEPEVNPLYRAD
PVDLEFSVDQVDSVDQEGPMDQQDPENPVAPLDQAGLMDPVDPEDSVDLGAAGASAQPLQ
PSSPVAYDIISQELELMKKLKEQLEERTWLLHDAIQNQQNALELMMDHLQKQPNTLRHVV
IPDLQSSEAVPKKQQKQHAGQVKRPLPHPKDVKCFCGLSLSNSLKNTGELQEPCVAFNQQ
QLVQQEQHLKEQQRQLREQLQQLREQRKVQKQKKMQEKKKLQEQKMQEKKKLQEQRRQKK
KKLQERKKWQGQMLQKEPEEEQQKQQLQEQPLKHNVIVGNERVQICLQNPRDVSVPLCNH
PVRFLQAQPIVPVQRAAEQQPSGFYQDENCGQQEDESQSFYPEAYQGPPVNQLPLIDTSN
SEAISSSSIPQFPITSDSTISTLETPQDYIRLWQELSDSLGPVVQVNTWSCDEQGTLHGQ
PTYHQVQVSEVGVEGPPDPQAFQGPAAYQPDQMRSAEQTRLMPAEQRDSNKPC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable