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Explanation of Terms


Gene: Gene Symbol: 
Gene Name:  auxin response factor-like protein
Sequence             
Original Clone ID: 1191.1.25_GO.18701
Keyword: None
Species: Arabidopsis thaliana Columbia
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00084307

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1830nts         Open reading frame : 28 to 1857

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGAGTGTTGAA
GGTGATGATGATTTCGGAAGTTCTTCGTCAAGGTCTTATCAAGATCAACTATACACAGAG
CTATGGAAAGTTTGTGCAGGTCCATTAGTGGAAGTTCCTCGTGCTCAAGAGAGAGTTTTC
TACTTCCCTCAGGGTCACATGGAACAACTTGTGGCGTCAACTAATCAAGGAATCAATTCA
GAAGAAATACCTGTTTTTGATCTTCCTCCAAAGATACTTTGTCGAGTTCTTGATGTCACT
TTAAAGGCGGAGCATGAAACAGATGAGGTTTACGCTCAGATCACATTACAACCAGAGGAA
GATCAAAGTGAACCAACAAGTCTTGATCCACCTATTGTTGGACCAACTAAGCAAGAGTTT
CATTCGTTTGTTAAGATTTTAACGGCTTCAGATACAAGCACTCATGGTGGATTCTCTGTT
CTTCGTAAACACGCCACTGAATGCTTGCCTTCTTTGGATATGGCACAAGCTACTCCTACT
CAAGAACTTGTGACTAGAGATCTTCATGGCTTTGAATGGAGGTTTAAGCATATATTCAGA
GGACAACCACGGAGGCATTTGCTTACTACGGGTTGGAGTACATTTGTATCCTCGAAAAGA
CTTGTAGCTGGAGATGCTTTTGTGTTCTTGAGGGGTGAGAATGGGGATTTACGGGTTGGA
GTGAGACGATTAGCTCGGCATCAAAGCACAATGCCTACTTCGGTTATTTCAAGTCAGAGC
ATGCATTTGGGAGTTCTTGCTACAGCTTCTCATGCTGTGCGTACAACAACAATCTTTGTT
GTCTTTTACAAGCCTAGGATAAGCCAATTCATAGTTGGGGTGAACAAGTATATGGAAGCT
ATAAAGCATGGATTTTCTCTCGGTACCCGATTCAGAATGAGGTTTGAAGGAGAAGAGTCT
CCTGAGAGAATATTTACTGGTACGATTGTGGGAAGTGGAGATCTATCTTCACAATGGCCA
GCTTCTAAATGGAGGTCATTGCAGGTACAATGGGATGAGCCAACAACAGTTCAGAGACCA
GATAAAGTCTCACCATGGGAGATAGAGCCTTTCTTGGCAACTTCCCCAATTTCAACTCCT
GCTCAACAACCACAATCGAAATGCAAGCGGTCAAGACCCATCGAGCCATCAGTTAAAACA
CCAGCCCCACCTAGTTTCTTGTACAGCCTCCCTCAGAGCCAAGATTCCATTAATGCATCC
CTTAAACTGTTTCAAGATCCATCACTTGAGAGAATTTCAGGTGGATACTCCTCAAACAAC
AGCTTCAAACCCGAGACTCCTCCTCCTCCAACGAATTGTAGCTATAGGTTGTTTGGATTT
GATCTCACAAGCAATTCTCCTGCTCCAATCCCTCAAGACAAGCAACCGATGGATACTTGT
GGAGCTGCCAAGTGTCAAGAACCCATCACTCCAACCTCAATGAGTGAGCAGAAGAAGCAA
CAAACATCAAGAAGTCGAACTAAAGTGCAAATGCAAGGCATTGCGGTTGGTCGTGCGGTT
GATTTAACACTGTTGAAATCTTACGATGAACTGATTGATGAGCTTGAGGAGATGTTTGAG
ATTCAAGGACAGCTTCTTGCCCGAGACAAATGGATCGTTGTCTTCACTGATGATGAAGGA
GATATGATGCTTGCTGGTGATGATCCGTGGAATGAGTTTTGCAAGATGGCAAAGAAGATA
TTTATATATTCGAGCGATGAGGTTAAGAAAATGACAACGAAACTGAAGATTTCTTCGTCG
TTAGAGAATGAGGAATATGGTAATGAATCATTCGAAAATCGTAGTAGGGGGTGATAGGAC
CCAGCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1830
Mutations:
None
Discrepancy:
a496g,T166A;
                                

5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Protein
  • Clone
  • Experimental

Protein

SMART domain : B3 : B3 DNA binding domain
Structural Annotation Wiki : TOPSAN

Clone

Cloning Information : 273360
HIP Master Clone ID : 203228
Original Clone ID : 1191.1.25_GO.18701

Experimental

TargetTrack - Experimental data : GO.18701