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TM0602 (Thermotoga maritima) in pMH1 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TM0602
Gene Name:  iron-dependent transcriptional repressor
Sequence             
Original Clone ID: 7345
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH1               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
TmCD00084926

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 456nts         Open reading frame : 51 to 506

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGTTATCAC
CAACGGAAAAAAGGTATCTCCTCGCCGTTCTTTTAACAACGGAAGAGGGAAGCACGAGGC
TGAAGAGAGTATCGGACTTTTTGAAAGTGAAAATGCCCTCCGCGAAGCAGATTCTGGAAG
ACCTCGCATCGAAAAAACTGATAAACTACGTGAGAAGGGGCCCCATTTCCCTCACAAAAA
AGGGTACAGAACTTGCCCAGAAAGAACTGGAGCGCTTCAACAACCTGAAGGAGTTTTTGA
AGAAGATCCTGTTCCTCGATGAGGAAGAGGCAGAAAAGGGTGCGTGGGAGATCTTCTTCA
ACCTCGAGGAGAGTATCGCCGATAGGGTCGTTGATTTCATGAACTTCCTCACCCACTGTC
CACACATAACGCCGATATGCATAAAGGGCTTCAAAGAATATCTGGAAACAGGAGAGTTTC
CAACTCTTTGCAGACTCAGAAGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG
ATGAGAGAAGATTTTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 456
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DL41
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 282475
Recommended expression in: DL41
       DyNA Vector Map

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Vector Name: pMH1

pMH1 in advanced viewed

Synonyms: pMH-1
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.
Comments: None
Size (bp): 4048
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1887 2569
inducible trxn activator protein gene araC araC cds 3144 4019
promoter araC araC promoter 256 285
promoter pBAD pBAD promoter 250 277
selectable marker AmpR ampicillin resistance gene 1130 1789
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 794 820

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : TM0602
GENEID : 897681
Locus Tag : TM0602
Target GenBank: NP_228412

 

Protein

SMART domain : HTH_DTXR : Helix-turn-helix diphteria tox regulatory element
Structural Annotation Wiki : TOPSAN
Swissprot ID : Q9WZ71

Clone

Cloning Information : 276573
HIP Master Clone ID : 206059
Original Clone ID : 7345

Experimental

TargetTrack - Experimental data : 282475

Organism


TAX_ID : 243274
Species Specific ID: 897681