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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 7293
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH1               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
TmCD00084941

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 519nts         Open reading frame : 51 to 569

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGCCTAGAG
GAAGGAAGAAAACCCTGACACCTGCGCTCGAAGATTACCTTGCAGTGATTCAGGAGATAC
TTCAGAAACAACCCGCGGCGAGGGTGAGCACCATCGCGAGAAAGATGGGAGTGAGCCTTC
CAAGTGTGACGAATGCCATGAAAAGACTGGCGGAACTTGGATACGTCGAATACGAGAAGT
ATGGATACATAACCCTTACCGAGAAGGGTAAAAGAAGAGCAAGATCGTTGAGAGGTTCTC
AGAACAGACTCAGGAACTTTTTCTTCTACGTGATGGGAATACCTTCACCCACCGCGGAAA
AACTCTCGCGTCATTTCTCACACTTCCTGGATACGAAAACGAGAGAAAGATTCAAGAGAT
TTTACGACATAATGGTGAATTTCGATGAGAGCAAGGTGCAGGAACTGAAGGAATTTCTCG
AAGAGAGCAGAAGACTCGTGAACGTTCGGGAAATCCCTGAAGAAGCCAGAATTATGGAAG
AAGATGAAGAAGAGGAGGCTCAACCATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGG
CGGATGAGAGAAGATTTTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 519
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DL41
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Applicable
SolubleProtein: Tested_Not_Soluble
ProteinPurified: Protein_Purified
Find experimental protocols in 282383
Recommended expression in: DL41
       DyNA Vector Map

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Vector Name: pMH1

pMH1 in advanced viewed

Synonyms: pMH-1
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.
Comments: None
Size (bp): 4048
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1887 2569
inducible trxn activator protein gene araC araC cds 3144 4019
promoter araC araC promoter 256 285
promoter pBAD pBAD promoter 250 277
selectable marker AmpR ampicillin resistance gene 1130 1789
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 794 820

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

Target GenBank: AAD35595

 

Clone

Cloning Information : 276538
HIP Master Clone ID : 206024
Original Clone ID : 7293

Experimental

TargetTrack - Experimental data : 282383

Organism


Species Specific ID: TM0510