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thyX (Thermotoga maritima) in pMH1 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  thyX
Gene Name:  FAD-dependent thymidylate synthase
Sequence             
Original Clone ID: 7252
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH1               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 12211025
Title: Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolution.
Authors: PSI
Joint Center for Structural Genomics
TmCD00084968

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 663nts         Open reading frame : 51 to 713

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAGATCG
ATATCTTGGACAAAGGATTCGTTGAACTTGTGGATGTGATGGGAAACGACCTCTCCGCTG
TACGGGCTGCCCGCGTTTCTTTCGATATGGGTTTGAAAGACGAAGAAAGGGATAGACATC
TCATCGAATATCTCATGAAACACGGTCACGAGACACCTTTCGAACATATTGTCTTCACTT
TCCACGTGAAAGCCCCTATATTCGTGGCGAGGCAGTGGTTCAGACACAGGATCGCTTCTT
ACAACGAACTGAGCGGCAGATACTCGAAGCTCTCCTACGAATTCTACATTCCCTCTCCCG
AACGCCTGGAAGGGTACAAAACGACCATCCCTCCCGAACGGGTGACGGAGAAGATCTCAG
AAATAGTCGATAAAGCGTATCGAACGTATCTGGAGTTGATAGAAAGCGGTGTTCCTCGAG
AAGTGGCAAGGATAGTGCTCCCTTTGAATCTGTACACGAGGTTTTTCTGGACTGTGAACG
CAAGAAGTCTCATGAACTTTTTGAACCTGAGGGCAGATTCTCACGCTCAGTGGGAAATTC
AACAGTACGCTCTGGCAATCGCGAGAATTTTCAAAGAGAAGTGTCCCTGGACTTTTGAGG
CATTTCTTAAGTATGCTTATAAAGGAGATATCCTGAAGGAGGTACAGGTATAAGTTTAAA
CGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 663
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DL41
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: SolubleProtein: Protein_Soluble
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in 282322
Recommended expression in: DL41
       DyNA Vector Map

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Vector Name: pMH1

pMH1 in advanced viewed

Synonyms: pMH-1
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.
Comments: None
Size (bp): 4048
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1887 2569
inducible trxn activator protein gene araC araC cds 3144 4019
promoter araC araC promoter 256 285
promoter pBAD pBAD promoter 250 277
selectable marker AmpR ampicillin resistance gene 1130 1789
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 794 820

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : thyX
GENEID : 897468
Locus Tag : TM0449
Target GenBank: NP_228259

 

Protein

PDB - Structural Annotation : 1KQ4
Structural Annotation Wiki : TOPSAN
Swissprot ID : Q9WYT0
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 275797
HIP Master Clone ID : 205283
Original Clone ID : 7252

Experimental

Structural Biology Knowledgebase : SBKB (1KQ4)
TargetTrack - Experimental data : 282322

Organism


TAX_ID : 243274
Species Specific ID: 897468