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Detailed Vector Information: p15Tv-LIC

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: p15Tv-LIC              
Source: Midwest Center for Structural Genomics
Description: Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI).
Comments: None
Publications: None
Authors: PSI
Midwest Center for Structural Genomics
University of Toronto
EvNO00085127

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: p15Tv-LIC

p15Tv-LIC in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7p
Reverse:  T7t
Description: Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI).
Comments: None
Size (bp): 7746
Parent Vector: None
Properties: acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 1164 1846
negative selection marker sacB sacB cds 5901 7319
promoter T7 T7 promoter 5230 5249
recombination site LoxP LoxP sequence 5407 5440
repressor binding site LacO LacO sequence region 5249 5271
repressor protein gene lacI lacI cds 3764 4843
selectable marker AmpR ampicillin resistance gene 407 1066
trxn termination sequence T7 term T7 terminator sequence 7488 7534

 

Species Specific ID: None
Target GenBank: None