Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pDB.His.MBP
Source:
Berkeley Structural Genomics Center
Description:
"Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning."
Comments:
None
Publications:
PMID: 24806409
Title: C9orf72 hypermethylation protects against repeat expansion-associated pathology in ALS/FTD
Title: C9orf72 hypermethylation protects against repeat expansion-associated pathology in ALS/FTD
Authors:
Berkeley Structural Genomics Center
PSI
Lawrence Berkeley National Laboratory
PSI
Lawrence Berkeley National Laboratory
EvNO00085130
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Vector Name:
pDB.His.MBP
Synonyms:
pDBHMBP, pHM
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
6494
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Berkeley Structural Genomics Center
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1421
2103
promoter
T7
T7 promoter
4984
5003
protease cleavage site
TEV
TEV protease cleavage site
6232
6249
repressor binding site
lac operator
lacO sequence region
5003
5025
repressor protein gene
lacI
lacI coding region
3518
4597
selectable marker
KanR
kanamycin resistance gene
563
1375
ssDNA origin
f1 ori
f1 origin
29
335
tag
MBP
Maltose Binding Protein cds
5071
6369
tag
His
6xHis tag
5083
5100
trxn termination sequence
T7 term
T7 terminator sequence
6423
6469
