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Detailed Vector Information: pDB.TRX

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pDB.TRX              
Source: Berkeley Structural Genomics Center
Description: Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
Comments: None
Publications: None
Authors: PSI
Berkeley Structural Genomics Center
Lawrence Berkeley National Laboratory
EvNO00085133

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
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Vector Name: pDB.TRX

pDB.TRX in advanced viewed

Synonyms: pTRX, pX
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5693
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin f1 F1 origin 29 335
bacterial origin ColE ColE1 origin 1421 2103
promoter T7 T7 promoter 4984 5003
protease cleavage site TEV TEV protease cleavage site cds 5431 5448
repressor binding site lac operator lac operator 5003 5025
repressor protein gene LacI LacI gene 2515 4594
selectable marker KanR kanamycin resistance gene 563 1375
tag TRX thioredoxin cds tag 5080 5403
trxn termination sequence T7 term T7 term sequencing primer 5610 5628

 

Species Specific ID: None
Target GenBank: None