| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pDB-HisGST |
| Source: | Berkeley Structural Genomics Center |
| Description: | "Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning." |
| Comments: | None |
| Publications: | None |
| Authors: |
Berkeley Structural Genomics Center PSI Lawrence Berkeley National Laboratory |
|
EvNO00085134 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
kanamycin Bacterial Selection Condition: 50ug/mL kanamycin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors. |
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| Vector Name: | pDB-HisGST |
pDB-HisGST in advanced viewed |
| Synonyms: | pDB.His.GST, pHG | |
| Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
| Description: | Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning. | |
| Comments: | None | |
| Size (bp): | 6053 | |
| Parent Vector: | None | |
| Properties: | bacterial expression, multiple cloning site, with tag/fusion/marker | |
| Author Name: |
Berkeley Structural Genomics Center |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | ori | ColE1-type bacterial origin of replication | 1421 | 2103 |
| promoter | T7 | T7 promoter | 4984 | 5003 |
| protease cleavage site | TEV | TEV protease cleavage site | 5791 | 5808 |
| repressor binding site | lacO | lacO sequence region | 5003 | 5025 |
| repressor protein gene | lacI | lacI coding region | 3518 | 4597 |
| selectable marker | KanR | kanamycin resistance gene | 1375 | 563 |
| ssDNA origin | f1 ori | f1 origin | 29 | 335 |
| tag | His | 6xHis tag | 5083 | 5100 |
| tag | GST | GST tag | 5104 | 5771 |
| trxn termination sequence | T7 term | T7 termination sequence | 5982 | 6028 |
| Species Specific ID: | None |
|---|---|
| Target GenBank: | None |
