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Detailed Vector Information: pDB-HisGST

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pDB-HisGST              
Source: Berkeley Structural Genomics Center
Description: "Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning."
Comments: None
Publications: None
Authors: PSI
Berkeley Structural Genomics Center
Lawrence Berkeley National Laboratory
EvNO00085134

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
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Vector Name: pDB-HisGST

pDB-HisGST in advanced viewed

Synonyms: pDB.His.GST, pHG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 6053
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1421 2103
promoter T7 T7 promoter 4984 5003
protease cleavage site TEV TEV protease cleavage site 5791 5808
repressor binding site lacO lacO sequence region 5003 5025
repressor protein gene lacI lacI coding region 3518 4597
selectable marker KanR kanamycin resistance gene 1375 563
ssDNA origin f1 ori f1 origin 29 335
tag GST GST tag 5104 5771
tag His 6xHis tag 5083 5100
trxn termination sequence T7 term T7 termination sequence 5982 6028

 

Species Specific ID: None
Target GenBank: None