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PSMB8 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PSMB8
Gene Name:  proteasome (prosome, macropain) subunit, beta type, 8
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH020022.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00000858

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 819nts         Open reading frame : 1 to 819
Reference Sequence Alignment

ATGCTCATAGGAACCCCCACCCCGCGTGACACTACTCCCAGCTCCTGGCTGACTTCTAGT
CTTCTGGTTGAAGCTGCGCCTTTAGATGACACGACCCTACCCACCCCTGTTTCCAGCGGA
TGCCCGGGCCTGGAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGACGGAGAAAGGAAC
GTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTCCAGCATGGAGTG
ATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCCTTACGGGTGAAC
AAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGTGCAGCAGACTGT
CAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTGCGAAATGGAGAA
CGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGCCAGTACCGGGGC
ATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGTCCTGGACTCTAC
TACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACGGGTAGTGGGAAC
ACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGCCCTGAAGAGGCC
TATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGCTATTCTGGAGGC
GTTGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAAAGTACAGATGTC
AGTGACCTGCTGCACCAGTACCAGGAAGCCAATCAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1039
Start on reference sequence 221
Mutations:
None
Discrepancy:
t615g; g803a,R268Q
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PSMB8
Symbol Nomenclature : PSMB8
SYNONYM : ALDD; D6S216; D6S216E; JMP; LMP7; NKJO; PSMB5i; RING10
Designation : large multifunctional peptidase 7|low molecular mass protein 7|low molecular weight protein 7|macropain subunit C13|multicatalytic endopeptidase complex subunit C13|protease component C13|proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)|proteasome catalytic subunit 3i|proteasome component C13|proteasome subunit Y2|proteasome subunit beta 5i|proteasome subunit beta type-8|proteasome-related gene 7|really interesting new gene 10 protein
Full Nomenclature : proteasome (prosome, macropain) subunit, beta type, 8
GENEID : 5696
Locus Tag : DAAP-57C1.3
GI : 61357316
GenBank Accession : AY888427
HGNC : 9545
MIM : 177046
Vega : OTTHUMG00000031285
Target GenBank: NM_004159

 

Reference Sequence Alignment

  

HsCD00000858      ------------ATGCTCATAGGAACCCCCACCCCGCGTGACACTACTCCCAGCTCCTGG
NM_004159.4       ------------ATGCTCATAGGAACCCCCACCCCGCGTGACACTACTCCCAGCTCCTGG
NM_148919.3       ATGGCGCTACTAGATGTATGCGGAGCCCCCCGAGGGCAGCGGCCGGAATCGGCTCTCCCG
                              .:  *.: .***.*****. .  **.  . .* ..: * .    *  *

HsCD00000858      CTGACTTCTAGTCTTCTGGTTGAAGCTGCGCCTTTAGATGACACGACCCTACCCACCCCT
NM_004159.4       CTGACTTCTAGTCTTCTGGTTGAAGCTGCGCCTTTAGATGACACGACCCTACCCACCCCT
NM_148919.3       GTTGCGGGAAGCGGGCGTCGCTCGGACCCAGGACACTACAGTTTCTCTATGCGATCTCCA
                   * .*   :**    *      ..*.  *.  : :. * .. :  :* .*.* .:* **:

HsCD00000858      GTTTCCAGCGGATGCCCGGGCCTGGAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGAC
NM_004159.4       GTTTCCAGCGGATGCCCGGGCCTGGAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGAC
NM_148919.3       GAGCTCGCTTTACCCCGGGGAATGCAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGAC
                  *:   *.    *  ** ***..** ***********************************

HsCD00000858      GGAGAAAGGAACGTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTC
NM_004159.4       GGAGAAAGGAACGTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTC
NM_148919.3       GGAGAAAGGAACGTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTC
                  ************************************************************

HsCD00000858      CAGCATGGAGTGATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCC
NM_004159.4       CAGCATGGAGTGATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCC
NM_148919.3       CAGCATGGAGTGATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCC
                  ************************************************************

HsCD00000858      TTACGGGTGAACAAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGT
NM_004159.4       TTACGGGTGAACAAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGT
NM_148919.3       TTACGGGTGAACAAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGT
                  ************************************************************

HsCD00000858      GCAGCAGACTGTCAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTG
NM_004159.4       GCAGCAGACTGTCAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTG
NM_148919.3       GCAGCAGACTGTCAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTG
                  ************************************************************

HsCD00000858      CGAAATGGAGAACGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGC
NM_004159.4       CGAAATGGAGAACGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGC
NM_148919.3       CGAAATGGAGAACGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGC
                  ************************************************************

HsCD00000858      CAGTACCGGGGCATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGT
NM_004159.4       CAGTACCGGGGCATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGT
NM_148919.3       CAGTACCGGGGCATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGT
                  ************************************************************

HsCD00000858      CCTGGACTCTACTACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACG
NM_004159.4       CCTGGACTCTACTACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACG
NM_148919.3       CCTGGACTCTACTACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACG
                  ************************************************************

HsCD00000858      GGTAGTGGGAACACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGC
NM_004159.4       GGTAGTGGGAACACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGC
NM_148919.3       GGTAGTGGGAACACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGC
                  ************************************************************

HsCD00000858      CCTGAAGAGGCCTATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGC
NM_004159.4       CCTGAAGAGGCCTATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGC
NM_148919.3       CCTGAAGAGGCCTATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGC
                  ************************************************************

HsCD00000858      TATTCTGGAGGCGTTGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAA
NM_004159.4       TATTCTGGAGGCGTTGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAA
NM_148919.3       TATTCTGGAGGCGTTGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAA
                  ************************************************************

HsCD00000858      AGTACAGATGTCAGTGACCTGCTGCACCAGTACCAGGAAGCCAATCAATAG
NM_004159.4       AGTACAGATGTCAGTGACCTGCTGCACCAGTACCGGGAAGCCAATCAATAA
NM_148919.3       AGTACAGATGTCAGTGACCTGCTGCACCAGTACCGGGAAGCCAATCAATAA
                  **********************************.***************.

Pathways

Reactome : APC/C-mediated degradation of cell cycle proteins
Reactome : APC/C:Cdc20 mediated degradation of Securin
Reactome : APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
Reactome : AUF1 (hnRNP D0) destabilizes mRNA
Reactome : Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : Activation of NF-kappaB in B cells
Reactome : Adaptive Immune System
Reactome : Antigen processing-Cross presentation
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Apoptosis
Reactome : Assembly of the pre-replicative complex
Reactome : Asymmetric localization of PCP proteins
Reactome : Autodegradation of Cdh1 by Cdh1:APC/C
Reactome : Autodegradation of the E3 ubiquitin ligase COP1
Reactome : CDK-mediated phosphorylation and removal of Cdc6
Reactome : CDT1 association with the CDC6:ORC:origin complex
Reactome : Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Cross-presentation of soluble exogenous antigens (endosomes)
Reactome : Cyclin A:Cdk2-associated events at S phase entry
Reactome : Cyclin E associated events during G1/S transition
Reactome : Cytokine Signaling in Immune system
Reactome : DNA Replication
Reactome : DNA Replication Pre-Initiation
Reactome : Disease
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : ER-Phagosome pathway
Reactome : G1/S DNA Damage Checkpoints
Reactome : G1/S Transition
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : Host Interactions of HIV factors
Reactome : Immune System
Reactome : Interferon Signaling
Reactome : Interferon alpha/beta signaling
Reactome : M Phase
Reactome : M/G1 Transition
Reactome : Metabolism
Reactome : Metabolism of RNA
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of mRNA
Reactome : Mitotic Anaphase
Reactome : Mitotic G1-G1/S phases
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Orc1 removal from chromatin
Reactome : PCP/CE pathway
Reactome : Regulation of APC/C activators between G1/S and early anaphase
Reactome : Regulation of Apoptosis
Reactome : Regulation of DNA replication
Reactome : Regulation of activated PAK-2p34 by proteasome mediated degradation
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome : Regulation of mitotic cell cycle
Reactome : Regulation of ornithine decarboxylase (ODC)
Reactome : Removal of licensing factors from origins
Reactome : S Phase
Reactome : SCF(Skp2)-mediated degradation of p27/p21
Reactome : SCF-beta-TrCP mediated degradation of Emi1
Reactome : Separation of Sister Chromatids
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Stabilization of p53
Reactome : Switching of origins to a post-replicative state
Reactome : Synthesis of DNA
Reactome : TCF dependent signaling in response to WNT
Reactome : Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
Reactome : Ubiquitin-dependent degradation of Cyclin D
Reactome : Ubiquitin-dependent degradation of Cyclin D1
Reactome : Vif-mediated degradation of APOBEC3G
Reactome : Vpu mediated degradation of CD4
Reactome : beta-catenin independent WNT signaling
Reactome : degradation of AXIN
Reactome : degradation of DVL
Reactome : p53-Dependent G1 DNA Damage Response
Reactome : p53-Dependent G1/S DNA damage checkpoint
Reactome : p53-Independent DNA Damage Response
Reactome : p53-Independent G1/S DNA damage checkpoint
WikiPathway : Proteasome Degradation

Protein

UniProt : P28062
HPRD : 01515

Clone

Cloning Information : 20022
HIP Master Clone ID : 34532
Original Clone ID : FLH020022.01X

Organism


TAX_ID : 9606
Species Specific ID: 5696

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000204264

Reagents

Labome : PSMB8-antibody