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Detailed Vector Information: pMK-Dest

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMK-Dest              
Source: None
Description: with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
Comments: None
Publications: None
Authors: Center for Personalized Diagnostics
Bianca
Mark Knappenberger
Krishna
EvNO00877066

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AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Host Type:  bacterial    Marker:  neomycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
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Vector Name: pMK-Dest

pMK-Dest in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
Comments: From Karen Anderson Lab, Produced by Bianca Varda, Krishna, and Mark Knappenberger
Size (bp): 3386
Parent Vector: Name: pMax-Cloning
Description:
Properties: Gateway, bacterial expression, with tag/fusion/marker
Author Name: Center for Personalized Diagnostics
Bianca
Mark Knappenberger
Krishna
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
CDS TEV TEV Cleavage site 1252 1272
Origin pBR322 origin 2748 2130
primer CMV-F 949 969
promoter CMV 938 1019
promoter lac promoter 1437 1460
recombination site attR1 1288 1412
recombination site attR2 1412 1312
recombination site AttB2 2066 2090
selectable marker NeoR Neomycin/Kanamycin Resistance 2816 209

 

Species Specific ID: None
Target GenBank: None