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TM0570 (Thermotoga maritima) in pMH4 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TM0570
Gene Name:  cell division protein FtsY
Sequence             
Original Clone ID: 13204
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
TmCD00089981

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 885nts         Open reading frame : 51 to 935

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGGACTCT
TCGATTTTTTGAAAAAGGGACTCCAGAAGACGAAAGAAACGTTTTTTGGACGAGTGGTGA
AGCTCCTCAAAGGAAAAAAGCTGGACGATGAAACCCGCGAGGAGCTGGAAGAACTTCTCA
TTCAAGCGGACGTGGGAGTCGAGACAACGGAATACATTCTGGAGAGATTGGAAGAGAAGG
ACGGAGACGCTCTCGAGTCTCTCAAGGAAATCATTCTGGAAATTCTGAACTTCGATACGA
AGCTGAACGTTCCTCCTGAACCCCCGTTCGTGATCATGGTTGTTGGTGTCAACGGAACGG
GAAAGACCACCTCCTGTGGAAAGCTCGCAAAGATGTTCGTGGATGAAGGAAAGAGTGTGG
TGCTCGCTGCGGCGGATACTTTCAGAGCAGCCGCCATAGAACAGCTGAAGATATGGGGAG
AACGTGTCGGTGCCACGGTGATATCCCACTCGGAAGGAGCAGATCCGGCCGCCGTTGCCT
TTGACGCGGTGGCTCACGCTCTCGCCAGAAACAAAGATGTGGTCATAATAGACACCGCCG
GAAGACTTCACACGAAGAAGAATCTCATGGAGGAATTGCGGAAGGTTCACAGAGTTGTCA
AGAAGAAGATCCCCGACGCTCCGCACGAAACGCTCCTCGTGATAGACGCCACCACCGGCC
AAAACGGACTTGTTCAGGCAAAAATATTCAAAGAAGCAGTGAATGTCACCGGTATAATTC
TCACAAAGCTCGACGGAACCGCGAAAGGCGGAATAACTCTGGCCATTGCCCGGGAACTGG
GAATTCCCATAAAGTTCATCGGTGTCGGAGAAAAGGCAGAAGACCTGAGGCCTTTCGATC
CTGAGGCCTTTGTGGAGGTATTGCTTTCAGAATAACGCGACTTAATTAACTCTAGATAAC
TGAGAAGGAGATATACATACCCGGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 935
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: SolubleProtein: Protein_Soluble
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in 282443
Recommended expression in: HK100
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : TM0570
GENEID : 897634
Locus Tag : TM0570
GI : 15643336
GenBank Accession : NP_228380.1
Target GenBank: NP_228380

 

Protein

PDB - Structural Annotation : 1VMA
SMART domain : SRP54 : SRP54-type protein, GTPase domain
SMART domain : SRP54_N : SRP54-type protein, helical bundle domain
SMART domain : AAA : ATPases associated with a variety of cellular activities
Structural Annotation Wiki : TOPSAN
Swissprot ID : Q9WZ40
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 454196
HIP Master Clone ID : 383682
Original Clone ID : 13204

Experimental

Structural Biology Knowledgebase : SBKB (1VMA)
TargetTrack - Experimental data : 282443

Organism


TAX_ID : 243274
Species Specific ID: 897634