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TM1095 (Thermotoga maritima) in pMH4 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TM1095
Gene Name:  adenylosuccinate lyase
Sequence             
Original Clone ID: 14589
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
TmCD00089990

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1296nts         Open reading frame : 51 to 1346

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGTAGAAA
GGTATTCGCTGTCACCGATGAAGGATCTCTGGACGGAGGAAGCGAAGTACAGAAGATGGC
TGGAAGTGGAACTGGCAGTGACGAGGGCGTACGAAGAACTGGGAATGATTCCGAAGGGAG
TCACAGAGAGGATAAGAAACAACGCTAAGATAGATGTGGAACTGTTCAAAAAGATAGAAG
AGAAAACAAACCACGATGTGGTGGCGTTCGTCGAAGGAATCGGCTCGATGATAGGAGAAG
ACTCGAGATTCTTCCACTACGGTCTCACCTCGTCAGATGTTCTCGACACCGCAAATTCCC
TCGCTCTCGTGGAAGCGGGGAAAATCCTGTTAGAGTCTCTAAAAGAGTTCTGCGACGTGC
TCTGGGAAGTCGCGAATCGGTACAAACACACTCCCACCATCGGAAGAACACACGGAGTCC
ACGCGGAACCCACCTCCTTTGGCTTGAAGGTCCTTGGATGGTACTCCGAAATGAAAAGGA
ACGTTCAGCGCCTTGAGAGGGCGATAGAGGAAGTTTCTTACGGTAAGATCAGTGGAGCTG
TTGGAAACTACGCAAACGTACCACCGGAGGTGGAGGAAAAAGCGCTTTCGTACCTGGGCT
TGAAACCTGAACCCGTCTCCACACAGGTTGTACCGAGGGATCGTCACGCTTTCTATCTGT
CCACGCTCGCGATCGTGGCAGCGGGGATAGAAAGGATCGCTGTCGAGATCAGACATCTTC
AGAGAACGGAAGTGCTCGAGGTGGAAGAACCGTTCAGGAAAGGTCAGAGGGGCTCGAGTG
CCATGCCCCACAAGAAGAATCCCATCACCTGCGAGCGGCTCACGGGACTTTCCAGAATGA
TGAGGGCGTACGTCGATCCTTCCCTTGAAAACATCGCGCTCTGGCATGAGAGGGACATTT
CGCACTCTTCCGTCGAAAGATACGTTTTTCCTGACGCCACGCAGACTCTTTACTACATGA
TCGTCACGGCCACAAACGTGGTGAGGAACATGAAAGTGAACGAAGAAAGAATGAAGAAGA
ACATAGATCTCACGAAAGGTCTCGTGTTCTCCCAGAGAGTCCTGCTGAAACTCATAGAGA
AAGGACTCACGAGGAAAGAGGCTTACGATATCGTCCAGAGGAACGCTCTGAAAACCTGGA
ATTCCGAAAAACACTTCCTCGAGTACCTCCTCGAGGATGAAGAGGTTAAGAAACTGGTTA
CCAAAGAAGAGCTCGAAGAACTCTTCGATATCTCTTACTACTTGAAACACGTAGACCACA
TCTTCGAACGCTTTGAAAAGGAGTAACGCGACTTAATTAACTCTAGATAACTGAGAAGGA
GATATACATACCCGGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1346
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: SolubleProtein: Tested_Not_Soluble
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
Find experimental protocols in 282961
Recommended expression in: DL41
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : TM1095
GENEID : 898670
Locus Tag : TM1095
GI : 15643852
GenBank Accession : NP_228901.1
Target GenBank: NP_228901

 

Protein

SMART domain : ADSL_C : Adenylosuccinate lyase C-terminus
Structural Annotation Wiki : TOPSAN
Swissprot ID : Q9X0I0

Clone

Cloning Information : 454252
HIP Master Clone ID : 383738
Original Clone ID : 14589

Experimental

TargetTrack - Experimental data : 282961

Organism


TAX_ID : 243274
Species Specific ID: 898670