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TM0755 (Thermotoga maritima) in pMH4 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TM0755
Gene Name:  hypothetical protein
Sequence             
Original Clone ID: 14579
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 22990880
Title: Histidine ligand variants of a flavo-diiron protein: effects on structure and activities
Authors: PSI
Joint Center for Structural Genomics
TmCD00089997

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1197nts         Open reading frame : 51 to 1247

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGCCAAAAA
TCTGGACTGAAAGGATCTTCGACGATCCGGAAATCTACGTTTTGAGGATAGATGATGACC
GAATAAGATACTTCGAAGCGGTCTGGGAAATACCCGAAGGGATCAGCTACAACGCATATC
TTGTGAAGCTGAATGGTGCAAACGTTCTGATAGACGGCTGGAAGGGAAACTACGCAAAAG
AGTTCATCGACGCTCTGTCCAAGATCGTTGATCCCAAAGAGATCACGCACATCATCGTAA
ACCACACCGAGCCGGACCACAGCGGATCTCTCCCCGCAACACTCAAAACGATTGGACACG
ATGTGGAAATAATCGCTTCGAACTTCGGAAAAAGACTTCTCGAAGGCTTCTACGGCATAA
AAGACGTGACTGTCGTGAAGGATGGAGAAGAGAGAGAAATCGGAGGAAAAAAATTCAAAT
TCGTCATGACTCCATGGCTTCACTGGCCCGACACGATGGTGACGTATCTTGATGGAATTC
TCTTCAGCTGTGATGTGGGTGGTGGATACCTGCTCCCAGAGATCCTGGACGACTCAAACG
AGAGCGTTGTGGAAAGGTACCTTCCCCACGTCACAAAGTACATCGTCACGGTGATAGGCC
ATTACAAGAATTACATCCTCGAAGGTGCGGAAAAGCTCTCCAGTCTGAAGATAAAGGCGC
TCCTTCCAGGGCACGGACTGATCTGGAAGAAAGATCCTCAGAGACTTTTGAATCACTACG
TGAGCGTTGCAAAGGGAGATCCGAAAAAAGGAAAGGTCACGGTGATCTACGATTCCATGT
ACGGCTTCGTCGAGAACGTGATGAAGAAGGCGATCGACTCTCTCAAGGAGAAAGGTTTCA
CACCGGTTGTGTACAAATTCTCCGACGAAGAGAGACCCGCGATCAGCGAGATCCTGAAAG
ACATCCCGGACAGTGAAGCACTGATCTTCGGTGTTTCCACCTACGAAGCAGAGATACATC
CACTCATGAGATTCACCCTTCTCGAGATAATAGATAAAGCGAACTACGAAAAACCCGTCC
TCGTCTTTGGAGTGCACGGCTGGGCTCCATCCGCAGAAAGAACAGCAGGAGAGCTTCTGA
AAGAAACGAAGTTCAGAATTCTCTCCTTCACGGAGATAAAAGGTTCAAACATGGATGAGA
GAAAGATCGAAGAGGCGATATCTCTTCTCAAAAAAGAACTGGAGTAACGCGACTTAATTA
ACTCTAGATAACTGAGAAGGAGATATACATACCCGGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1247
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282625
Recommended expression in: DL41
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : TM0755
GENEID : 898423
Locus Tag : TM0755
GI : 15643518
GenBank Accession : NP_228564.1
Target GenBank: NP_228564

 

Protein

PDB - Structural Annotation : 1VME
SMART domain : Lactamase_B : Metallo-beta-lactamase superfamily
Structural Annotation Wiki : TOPSAN
Swissprot ID : Q9WZL4
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 454266
HIP Master Clone ID : 383752
Original Clone ID : 14579

Experimental

Structural Biology Knowledgebase : SBKB (1VME)
TargetTrack - Experimental data : 282625

Organism


TAX_ID : 243274
Species Specific ID: 898423