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SH3GL2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SH3GL2
Gene Name:  SH3-domain GRB2-like 2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH007590.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000902

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1059nts         Open reading frame : 1 to 1059

ATGTCGGTGGCCGGCCTCAAGAAGCAGTTCCATAAAGCCACTCAGAAAGTGAGTGAGAAG
GTTGGAGGAGCTGAAGGAACCAAGCTAGATGATGACTTCAAAGAGATGGAAAGGAAAGTG
GATGTCACCAGCAGGGCTGTGATGGAAATAATGACTAAAACAATTGAATACCTTCAACCC
AATCCAGCTTCCAGAGCTAAGCTCAGCATGATCAACACCATGTCAAAAATCCGTGGCCAG
GAGAAGGGGCCAGGCTATCCTCAGGCAGAGGCGCTGCTGGCAGAGGCCATGCTCAAATTT
GGAAGAGAGCTTGGAGATGATTGCAACTTTGGCCCAGCACTTGGTGAGGTCGGGGAGGCC
ATGCGGGAACTGTCGGAGGTCAAAGACTCTTTGGACATAGAAGTGAAGCAGAACTTCATT
GACCCTCTTCAGAATCTTCATGACAAAGATCTCAGGGAAATTCAACATCATCTAAAGAAG
TTGGAGGGTCGACGCCTGGATTTTGATTATAAGAAGAAACGACAAGGCAAGATTCCGGAT
GAAGAGCTTCGTCAAGCTCTAGAGAAATTTGATGAGTCTAAGGAAATTGCTGAGTCAAGC
ATGTCCAATCTCTTGGAGATGGATATTGAACAAGTGAGCCAGCTCTCTGCACTTGTGCAA
GCTCAGCTGGAGTACCACAAGCAGGCAGTCCAGATCCTGCAGCAAGTCACGGTCAGACTG
GAAGAAAGAATAAGACAGGCTTCATCTCAGCCTAGAAGGGAATATCAACCTAAACCACGA
ATGAGCCTGGAGTTTCCAACTGGAGACAGTACTCAGCCCAATGGGGGTCTCTCCCACACA
GGCACTCCCAAACCTTCAGGTGTCCAAATGGATCAGCCCTGCTGCCGAGCTCTGTACGAC
TTTGAACCTGAAAATGAAGGGGAGTTGGGATTTAAAGAGGGCGATATCATCACACTCACT
AACCAAATTGATGAGAACTGGTATGAGGGGATGCTGCATGGCCATTCAGGCTTCTTCCCC
ATCAATTATGTGGAAATTCTGGTTGCCCTGCCCCATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1106
Start on reference sequence 48
Mutations:
None
Discrepancy:
t453c; t605c,F202S
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 


Cloning Information: 7590
GI: 61357455
GenBank Accession: AY888447
HIP Master Clone ID: 7148
Labome: SH3GL2-antibody
Original Clone ID: FLH007590.01X
SMART domain: BAR :
SMART domain: SH3 : Src homology 3 domains
UniProt: Q7Z376
UniProt: Q8IZ09
UniProt: Q99962
Species Specific ID: 6456
Target GenBank: NM_003026