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UBE3A (Homo sapiens) in pGEX-2T

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Explanation of Terms


Gene: Gene Symbol:  UBE3A
Gene Name:  ubiquitin protein ligase E3A
Sequence              Map: Howley04028.pdf
Original Clone ID: 04028
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEX-2T               Format:  CLOSED
Source: Howley Lab
Description: None
Comments: Howley lab states, This is probably the clone that was used in the PNAS paper, but that cannot be confirmed. The thrombin site is still intact. To clone into pGEX2T the 3p EcoRI site was cut, filled in and blunt ligated to the vector, and therefore the site was destroyed.
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 10449731
Title: Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination
Authors: Rebecca Kramer
Peter Howley
HsCD00009059

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEX-2T

pGEX-2T in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor, thrombin cleavage and kinase site ORFs; amp resistance; restriction enzyme cloning.
Comments: None
Size (bp): 4900
Parent Vector: None
Empty Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Amersham Pharmacia
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS BamHI, SmaI, EcoRI sites for in-frame cloning 0 0
bacterial origin ColE1 pBR322-type ColE1 origin 0 0
phosphorylation site Kinase site Kinase site (phosphorylation target) Arg Arg Ala Ser Val 0 0
promoter Ptac Ptac promoter 0 0
protease cleavage site Thrombin Thrombin protease cleavage site Leu Val Pro Arg (cut) Gly Ser 0 0
selectable marker AmpR ampicillin resistance gene 0 0
tag GST glutathione S-transferase (GST) coding region 0 0

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : UBE3A
Symbol Nomenclature : UBE3A
SYNONYM : ANCR; AS; E6-AP; EPVE6AP; HPVE6A
Designation : CTCL tumor antigen se37-2|E6AP ubiquitin-protein ligase|human papilloma virus E6-associated protein|human papillomavirus E6-associated protein|oncogenic protein-associated protein E6-AP|renal carcinoma antigen NY-REN-54|ubiquitin-protein ligase E3A
Full Nomenclature : ubiquitin protein ligase E3A
GENEID : 7337
SYNONYM : pGEX-2T-E6AP-95kD
HGNC : 12496
MIM : 601623
Vega : OTTHUMG00000171548
Target GenBank: None

 

Pathways

NCI : Coregulation of Androgen receptor activity
NCI : Regulation of Telomerase
Panther : Ubiquitin proteasome pathway
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System
WikiPathway : Androgen receptor signaling pathway
WikiPathway : Tryptophan metabolism

Protein

HPRD : 03375

Clone

Original Clone ID : 04028

Organism


TAX_ID : 9606
Species Specific ID: 7337

Genome

Chromosome : 15
Map Location : 15q11.2
Ensembl : ENSG00000114062