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UBE3A (Homo sapiens) in pGEX-2T


Explanation of Terms

Gene: Gene Symbol:  UBE3A
Gene Name:  ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome)
Sequence              Map: Howley04028.pdf
Original Clone ID: 04028
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEX-2T               Format:  CLOSED
Source: Howley Lab
Description: None
Comments: Howley lab states, This is probably the clone that was used in the PNAS paper, but that cannot be confirmed. The thrombin site is still intact. To clone into pGEX2T the 3p EcoRI site was cut, filled in and blunt ligated to the vector, and therefore the site was destroyed.
Discrepancy :
No / Unknown
Publications: PMID: 10449731
Title: Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination
Authors: Rebecca Kramer
Peter Howley

Price:  Login for Pricing
CPD only
Special MTA: None


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
5' Linker Sequence: None
3' Linker Sequence: None


 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEX-2T

pGEX-2T in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor, thrombin cleavage and kinase site ORFs; amp resistance; restriction enzyme cloning.
Comments: None
Size (bp): 4900
Parent Vector: None
Empty Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Amersham Pharmacia
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS BamHI, SmaI, EcoRI sites for in-frame cloning 0 0
bacterial origin ColE1 pBR322-type ColE1 origin 0 0
phosphorylation site Kinase site Kinase site (phosphorylation target) Arg Arg Ala Ser Val 0 0
promoter Ptac Ptac promoter 0 0
protease cleavage site Thrombin Thrombin protease cleavage site Leu Val Pro Arg (cut) Gly Ser 0 0
selectable marker AmpR ampicillin resistance gene 0 0
tag GST glutathione S-transferase (GST) coding region 0 0


Original Clone ID: 04028
Species Specific ID: 7337
Target GenBank: None