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UBE2D1 (Homo sapiens) in pET-3a

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Explanation of Terms


Gene: Gene Symbol:  UBE2D1
Gene Name:  ubiquitin-conjugating enzyme E2D 1
Sequence              Map: Howley03992.pdf
Original Clone ID: 03992-01
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET-3a               Format:  CLOSED
Source: Howley Lab
Description: None
Comments: Howley lab states, Original clone from Martin Scheffner. Sushant successfully expressed in HB101 cells, and Novagen recommends using BL21s.
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 8090726
Title: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53
Authors: Rebecca Kramer
Peter Howley
HsCD00009066

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pET-3a

pET-3a in advanced viewed

Synonyms: pET3a
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pET-series prokaryotic expression/cloning vector with T7, T7 tag, T7 primer site; ampicillin resistance; restriction enzyme cloning.
Comments: Probably related to Novagen pET series vectors but not certain.
Size (bp): 4640
Parent Vector: None
Empty Vector: None
Properties: with tag/fusion/marker
Author Name: Novagen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE pBR322-type ColE1 origin 2814 2814
primer site 3' splice acceptor Recommended T7 primer sequence 615 633
promoter T7 T7 promoter 615 631
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 3676 4432
tag T7 tag T7 Tag (encodes short peptide sequence) 519 551
trxn termination sequence T7 term T7 terminator 404 450

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : UBE2D1
Symbol Nomenclature : UBE2D1
SYNONYM : E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A
Designation : UBC4/5 homolog|stimulator of Fe transport|ubiquitin carrier protein D1|ubiquitin-conjugating enzyme E2 D1|ubiquitin-conjugating enzyme E2(17)KB 1|ubiquitin-conjugating enzyme E2-17 kDa 1|ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)|ubiquitin-protein ligase D1
Full Nomenclature : ubiquitin-conjugating enzyme E2D 1
GENEID : 7321
SYNONYM : pET3a-UbcH5
SYNONYM : pET-3a-ubcH5
HGNC : 12474
MIM : 602961
Vega : OTTHUMG00000018269
Target GenBank: NM_003338

 

Pathways

NCI : Internalization of ErbB1
NCI : p53 pathway
Panther : Ubiquitin proteasome pathway
Reactome : APC-Cdc20 mediated degradation of Nek2A
Reactome : APC/C-mediated degradation of cell cycle proteins
Reactome : APC/C:Cdc20 mediated degradation of Cyclin B
Reactome : APC/C:Cdc20 mediated degradation of Securin
Reactome : APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
Reactome : Activated TLR4 signalling
Reactome : Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Autodegradation of Cdh1 by Cdh1:APC/C
Reactome : Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Cellular Senescence
Reactome : Cellular response to hypoxia
Reactome : Cellular responses to stress
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
Reactome : Disease
Reactome : Downregulation of SMAD2/3:SMAD4 transcriptional activity
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : IKK complex recruitment mediated by RIP1
Reactome : Immune System
Reactome : Inactivation of APC/C via direct inhibition of the APC/C complex
Reactome : Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
Reactome : Innate Immune System
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : M Phase
Reactome : Mitotic Anaphase
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Mitotic Spindle Checkpoint
Reactome : MyD88-independent cascade
Reactome : Negative regulators of RIG-I/MDA5 signaling
Reactome : Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
Reactome : Phosphorylation of the APC/C
Reactome : RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
Reactome : Regulation of APC/C activators between G1/S and early anaphase
Reactome : Regulation of Hypoxia-inducible Factor (HIF) by oxygen
Reactome : Regulation of mitotic cell cycle
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Senescence-Associated Secretory Phenotype (SASP)
Reactome : Separation of Sister Chromatids
Reactome : Signal Transduction
Reactome : Signaling by BMP
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer
Reactome : TRIF-mediated TLR3/TLR4 signaling
Reactome : Toll Like Receptor 3 (TLR3) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll-Like Receptors Cascades
Reactome : Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer
WikiPathway : Proteasome Degradation

Protein

HPRD : 04267

Clone

Original Clone ID : 03992-01

Organism


TAX_ID : 9606
Species Specific ID: 7321

Genome

Chromosome : 10
Map Location : 10q21.1
Ensembl : ENSG00000072401