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UBE2D1 (Homo sapiens) in pET-3a

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Explanation of Terms


Gene: Gene Symbol:  UBE2D1
Gene Name:  ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
Sequence              Map: Howley03992.pdf
Original Clone ID: 03992-01
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET-3a               Format:  CLOSED
Source: Howley Lab
Description: None
Comments: Howley lab states, Original clone from Martin Scheffner. Sushant successfully expressed in HB101 cells, and Novagen recommends using BL21s.
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 8090726
Title: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53
Authors: Rebecca Kramer
Peter Howley
HsCD00009066

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pET-3a

pET-3a in advanced viewed

Synonyms: pET3a
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pET-series prokaryotic expression/cloning vector with T7, T7 tag, T7 primer site; ampicillin resistance; restriction enzyme cloning.
Comments: Probably related to Novagen pET series vectors but not certain.
Size (bp): 4640
Parent Vector: None
Empty Vector: None
Properties: with tag/fusion/marker
Author Name: Novagen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE pBR322-type ColE1 origin 2814 2814
primer site 3' splice acceptor Recommended T7 primer sequence 615 633
promoter T7 T7 promoter 615 631
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 3676 4432
tag T7 tag T7 Tag (encodes short peptide sequence) 519 551
trxn termination sequence T7 term T7 terminator 404 450

 


Original Clone ID: 03992-01
SYNONYM: pET3a-UbcH5
SYNONYM: pET-3a-ubcH5
Species Specific ID: 7321
Target GenBank: NM_003338