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PTPN11 (Homo sapiens) in pBABEpuro

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Explanation of Terms


Gene: Gene Symbol:  PTPN11
Gene Name:  protein tyrosine phosphatase, non-receptor type 11
Sequence              Map: map256.pdf
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pBABEpuro               Format:  CLOSED
Source: Neel Lab
Description: None
Comments: None
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 10594032
Title: Activated mutants of SHP-2 preferrentially induce elongateion of Xenopus animal caps
Authors: Yongping Wang
Benjamin Neel
HsCD00009084

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
E76A

Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : T1 phage resistant DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pBABEpuro

pBABEpuro in advanced viewed

Synonyms: pBABE, pBABE-puro
Sequencing Primer: Forward:  pBABE 5'
Reverse:  pBABE 3'
Description: Mammalian retroviral vector; amp resistance (puromycin resistance for mammalian selection); restriction enzyme cloning.
Comments: Map and sequence from Stewart lab http://www.stewartlab.wustl.edu/html/plasmids.html; see also PMID 2194165 Morgenstern and Land (1990)
Size (bp): 5169
Parent Vector: None
Empty Vector: None
Properties: mammalian expression, mammalian transduction, retroviral vector
Author Name: JP Morgenstern
Publications: PMID: 2194165
Title: Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site (includes EcoRI at 1380 and SalI at 1398) 1375 1425
bacterial origin ColE ColE1 bacterial origin 3329 3357
promoter SV40 SV40 viral promoter 1546 1742
selectable marker PuroR puromycin resistance 1770 2430
selectable marker AmpR ampicillin resistance gene 4032 4892
splice donor site 5' splice donor mutant splice donor site 530 530
viral LTR 3pLTR 3' long terminal repeat 2470 3065
viral LTR 5pLTR 5' long terminal repeat 12 479

 

  • Gene
  • Pathways
  • Protein
  • Organism
  • Genome

Gene

 

Gene Symbol : PTPN11
Symbol Nomenclature : PTPN11
SYNONYM : BPTP3; CFC; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2
Designation : PTP-2C|protein-tyrosine phosphatase 1D|protein-tyrosine phosphatase 2C|tyrosine-protein phosphatase non-receptor type 11
Full Nomenclature : protein tyrosine phosphatase, non-receptor type 11
GENEID : 5781
SYNONYM : pBABE-puro-SHP2-E76A
SYNONYM : pBABEpuro-PTPN11-EA
SYNONYM : map256
HGNC : 9644
MIM : 176876
Vega : OTTHUMG00000134334
Target GenBank: NM_002834

 

Pathways

NCI : Angiopoietin receptor Tie2-mediated signaling
NCI : CXCR4-mediated signaling events
NCI : EGF receptor (ErbB1) signaling pathway
NCI : EPO signaling pathway
NCI : ErbB2/ErbB3 signaling events
NCI : FGF signaling pathway
NCI : Fc-epsilon receptor I signaling in mast cells
NCI : GMCSF-mediated signaling events
NCI : IFN-gamma pathway
NCI : IGF1 pathway
NCI : IL2 signaling events mediated by PI3K
NCI : IL2 signaling events mediated by STAT5
NCI : IL2-mediated signaling events
NCI : IL3-mediated signaling events
NCI : IL5-mediated signaling events
NCI : IL6-mediated signaling events
NCI : Insulin Pathway
NCI : Integrins in angiogenesis
NCI : N-cadherin signaling events
NCI : Neurotrophic factor-mediated Trk receptor signaling
NCI : PDGFR-beta signaling pathway
NCI : SHP2 signaling
NCI : Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI : Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI : Signaling events mediated by VEGFR1 and VEGFR2
NCI : Signaling events regulated by Ret tyrosine kinase
NCI : TCR signaling in naïve CD4+ T cells
NCI : VEGFR1 specific signals
Reactome : Activated TLR4 signalling
Reactome : Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
Reactome : Adaptive Immune System
Reactome : Axon guidance
Reactome : CTLA4 inhibitory signaling
Reactome : Cell surface interactions at the vascular wall
Reactome : Cell-Cell communication
Reactome : Constitutive PI3K/AKT Signaling in Cancer
Reactome : Costimulation by the CD28 family
Reactome : Cytokine Signaling in Immune system
Reactome : DAP12 interactions
Reactome : DAP12 signaling
Reactome : Developmental Biology
Reactome : Disease
Reactome : Downstream signal transduction
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : Downstream signaling of activated FGFR
Reactome : FRS2-mediated cascade
Reactome : Fc epsilon receptor (FCERI) signaling
Reactome : GAB1 signalosome
Reactome : Hemostasis
Reactome : IGF1R signaling cascade
Reactome : IRS-mediated signalling
Reactome : IRS-related events
Reactome : IRS-related events triggered by IGF1R
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Insulin receptor signalling cascade
Reactome : Interferon Signaling
Reactome : Interferon alpha/beta signaling
Reactome : Interferon gamma signaling
Reactome : Interleukin-3, 5 and GM-CSF signaling
Reactome : Interleukin-6 signaling
Reactome : MyD88-independent cascade
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : Negative regulation of FGFR signaling
Reactome : Netrin mediated repulsion signals
Reactome : Netrin-1 signaling
Reactome : Nuclear signaling by ERBB4
Reactome : PD-1 signaling
Reactome : PECAM1 interactions
Reactome : PI-3K cascade
Reactome : PI3K Cascade
Reactome : PI3K events in ERBB2 signaling
Reactome : PI3K events in ERBB4 signaling
Reactome : PI3K/AKT Signaling in Cancer
Reactome : PI3K/AKT activation
Reactome : PIP3 activates AKT signaling
Reactome : Platelet homeostasis
Reactome : Platelet sensitization by LDL
Reactome : Prolactin receptor signaling
Reactome : Regulation of IFNA signaling
Reactome : Regulation of IFNG signaling
Reactome : Role of LAT2/NTAL/LAB on calcium mobilization
Reactome : Signal Transduction
Reactome : Signal regulatory protein (SIRP) family interactions
Reactome : Signaling by EGFR
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by ERBB4
Reactome : Signaling by FGFR
Reactome : Signaling by FGFR in disease
Reactome : Signaling by Insulin receptor
Reactome : Signaling by Interleukins
Reactome : Signaling by Leptin
Reactome : Signaling by PDGF
Reactome : Signaling by SCF-KIT
Reactome : Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Signalling by NGF
Reactome : Spry regulation of FGF signaling
Reactome : TRIF-mediated TLR3/TLR4 signaling
Reactome : Tie2 Signaling
Reactome : Toll Like Receptor 3 (TLR3) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll-Like Receptors Cascades
WikiPathway : Alpha 6 Beta 4 signaling pathway
WikiPathway : B Cell Receptor Signaling Pathway
WikiPathway : EGF/EGFR Signaling Pathway
WikiPathway : Heart Development
WikiPathway : IL-1 signaling pathway
WikiPathway : IL-2 Signaling pathway
WikiPathway : IL-3 Signaling Pathway
WikiPathway : IL-4 signaling pathway
WikiPathway : IL-5 signaling pathway
WikiPathway : IL-6 signaling pathway
WikiPathway : Insulin Signaling
WikiPathway : Kit receptor signaling pathway
WikiPathway : Leptin signaling pathway
WikiPathway : Prolactin Signaling Pathway
WikiPathway : Proteins and DNA Sequences in Cardicac Structures
WikiPathway : Signaling of Hepatocyte Growth Factor Receptor
WikiPathway : TCR Signaling Pathway
WikiPathway : Type II interferon signaling (IFNG)
WikiPathway : angiogenesis overview

Protein

HPRD : 01470

Organism


TAX_ID : 9606
Species Specific ID: 5781

Genome

Chromosome : 12
Map Location : 12q24
Ensembl : ENSG00000179295