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PTPN11 (Homo sapiens) in pBABEpuro

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Explanation of Terms


Gene: Gene Symbol:  PTPN11
Gene Name:  protein tyrosine phosphatase, non-receptor type 11
Sequence              Map: map256.pdf
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pBABEpuro               Format:  CLOSED
Source: Neel Lab
Description: None
Comments: None
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 10594032
Title: Activated mutants of SHP-2 preferrentially induce elongateion of Xenopus animal caps
Authors: Yongping Wang
Benjamin Neel
HsCD00009084

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AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
E76A

Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : T1 phage resistant DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pBABEpuro

pBABEpuro in advanced viewed

Synonyms: pBABE, pBABE-puro
Sequencing Primer: Forward:  pBABE 5'
Reverse:  pBABE 3'
Description: Mammalian retroviral vector; amp resistance (puromycin resistance for mammalian selection); restriction enzyme cloning.
Comments: Map and sequence from Stewart lab http://www.stewartlab.wustl.edu/html/plasmids.html; see also PMID 2194165 Morgenstern and Land (1990)
Size (bp): 5169
Parent Vector: None
Empty Vector: None
Properties: mammalian expression, mammalian transduction, retroviral vector
Author Name: JP Morgenstern
Publications: PMID: 2194165
Title: Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site (includes EcoRI at 1380 and SalI at 1398) 1375 1425
bacterial origin ColE ColE1 bacterial origin 3329 3357
promoter SV40 SV40 viral promoter 1546 1742
selectable marker PuroR puromycin resistance 1770 2430
selectable marker AmpR ampicillin resistance gene 4032 4892
splice donor site 5' splice donor mutant splice donor site 530 530
viral LTR 3pLTR 3' long terminal repeat 2470 3065
viral LTR 5pLTR 5' long terminal repeat 12 479

 


SYNONYM: pBABE-puro-SHP2-E76A
SYNONYM: pBABEpuro-PTPN11-EA
SYNONYM: map256
Species Specific ID: 5781
Target GenBank: NM_002834