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PTPN11 (Homo sapiens) in pSp64RI

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Explanation of Terms


Gene: Gene Symbol:  PTPN11
Gene Name:  protein tyrosine phosphatase, non-receptor type 11
Sequence              Map: map235.pdf
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pSp64RI               Format:  CLOSED
Source: Neel Lab
Description: None
Comments: None
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 10594032
Title: Activated mutants of SHP-2 preferrentially induce elongateion of Xenopus animal caps
Authors: Yongping Wang
Benjamin Neel
HsCD00009092

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AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
E76A

Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : T1 phage resistant DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSp64RI

pSp64RI in advanced viewed

Synonyms: pSp64R1
Description: Eukaryotic (Xenopus-specific?) expression vector with X beta-globin 3p and 5p UTR; amp resistance; restriction enzyme cloning.
Comments: BamHI can be used to linearize plasmid for transcription with Sp6
Size (bp): 0
Parent Vector: Name: pSp64T
Description: pSp64T (PMID6700722) used to create pSp64RI (PMID7859288)
Empty Vector: None
Properties: in vitro transcription
Author Name: Terry Tang
Publications: PMID: 7859288
Title: The SH2-containing protein-tyrosine phosphatase SH-PTP2 is upstream of MAP kinase for early Xenopus development

Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
3pUTR globin 3pUTR Xenopus beta-globin 3 prime UTR (200bp flanked by XhoI PstI) 0 0
5pUTR globin 5pUTR Xenopus beta-globin 5 prime UTR (60bp flanked by HindII KpnI) 0 0
MCS MCS multiple cloning site of sorts use this to linearize EcoRI flanked inserts prior to in vitro transcription (PstI SalI XbaI BamHI SmaI SacI) 0 0
bacterial origin origin bacterial origin 0 0
selectable marker AmpR ampicillin resistance gene 0 0

 


SYNONYM: pSp64RI-SHP2-E76A
SYNONYM: map235
Species Specific ID: 5781
Target GenBank: NM_002834