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PTPN11 (Homo sapiens) in pJ3-omega

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Explanation of Terms


Gene: Gene Symbol:  PTPN11
Gene Name:  protein tyrosine phosphatase, non-receptor type 11
Sequence              Map: map249.pdf
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJ3-omega               Format:  CLOSED
Source: Neel Lab
Description: None
Comments: None
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 10594032
Title: Activated mutants of SHP-2 preferrentially induce elongateion of Xenopus animal caps
Authors: Yongping Wang
Benjamin Neel
HsCD00009095

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
E76A

Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : T1 phage resistant DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJ3-omega

pJ3-omega in advanced viewed

Synonyms: pJ3, pJ3-Omega, pJ3omega, pJ3Omega
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mammalian expression vector with SV40 promoter and intervening sequence and polyA; amp resistance; restriction enzyme cloning.
Comments: Best available related map NOT pJ3 map
Size (bp): 3500
Parent Vector: None
Empty Vector: None
Properties: mammalian expression
Author Name: JP Morgenstern
Publications: PMID: 2194165
Title: Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line

Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR ori pBR-type bacterial origin of replication 0 0
intervening sequence SV40 t IVS SV40 T intervening sequence 0 0
polyA site SV40 T poly A SV40 T polyadenylation site 0 0
promoter SV40 SV40 early promoter 0 0
selectable marker AmpR ampicillin resistance gene 0 0

 


SYNONYM: pJ3-SHP2-E-to-A
SYNONYM: map249
SYNONYM: pJ3Omega-SHP2-E-to-A
Species Specific ID: 5781
Target GenBank: NM_002834