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PTPN11 (Homo sapiens) in pSp64RI
Explanation of Terms
Clone: HsCD00009111
Vector: pSp64RI
Additional Annotations
Gene:
Gene Symbol:
PTPN11
Gene Name:
  protein tyrosine phosphatase, non-receptor type 11
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSp64RI
Format:
CLOSED
Source:
Neel Lab
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / Unknown
Publications:
PMID:
10594032
Title:
Activated mutants of SHP-2 preferrentially induce elongateion of Xenopus animal caps
Authors:
Benjamin Neel
Yongping Wang
HsCD00009111
Price:
Login for Pricing
UNAVAILABLE
Academic and non-profit labs
Special MTA: None
Insert Sequence
Protein Sequence
None
None
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
D61A
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
T1 phage resistant DH5-alpha
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pSp64RI
Synonyms:
pSp64R1
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Eukaryotic (Xenopus-specific?) expression vector with X beta-globin 3p and 5p UTR; amp resistance; restriction enzyme cloning.
Comments:
BamHI can be used to linearize plasmid for transcription with Sp6
Size (bp):
0
Parent Vector:
Name:
pSp64T
Description:
pSp64T (PMID6700722) used to create pSp64RI (PMID7859288)
Properties:
in vitro transcription
Author Name:
Terry Tang
Publications:
PMID:
7859288
Title:
The SH2-containing protein-tyrosine phosphatase SH-PTP2 is upstream of MAP kinase for early Xenopus development
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
3pUTR
globin 3pUTR
Xenopus beta-globin 3 prime UTR (200bp flanked by XhoI PstI)
0
0
5pUTR
globin 5pUTR
Xenopus beta-globin 5 prime UTR (60bp flanked by HindII KpnI)
0
0
MCS
MCS
multiple cloning site of sorts use this to linearize EcoRI flanked inserts prior to in vitro transcription (PstI SalI XbaI BamHI SmaI SacI)
0
0
bacterial origin
origin
bacterial origin
0
0
selectable marker
AmpR
ampicillin resistance gene
0
0
Gene
Pathways
Protein
Organism
Genome
Gene
Gene Symbol :
PTPN11
Symbol Nomenclature :
PTPN11
SYNONYM :
BPTP3; CFC; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2
Designation :
PTP-2C|protein-tyrosine phosphatase 1D|protein-tyrosine phosphatase 2C|tyrosine-protein phosphatase non-receptor type 11
Full Nomenclature :
protein tyrosine phosphatase, non-receptor type 11
GENEID :
5781
SYNONYM :
pSp64RI-SHP1-D61A
SYNONYM :
pSp64R1-SHP1-D61A
SYNONYM :
pSp64RI-SHP-1-D61A
HGNC :
9644
MIM :
176876
Vega :
OTTHUMG00000134334
Target GenBank:
None
Pathways
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
CXCR4-mediated signaling events
NCI :
EGF receptor (ErbB1) signaling pathway
NCI :
EPO signaling pathway
NCI :
ErbB2/ErbB3 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
FGF signaling pathway
NCI :
GMCSF-mediated signaling events
NCI :
IFN-gamma pathway
NCI :
IGF1 pathway
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL2 signaling events mediated by STAT5
NCI :
IL2-mediated signaling events
NCI :
IL3-mediated signaling events
NCI :
IL5-mediated signaling events
NCI :
IL6-mediated signaling events
NCI :
Insulin Pathway
NCI :
Integrins in angiogenesis
NCI :
N-cadherin signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
PDGFR-beta signaling pathway
NCI :
SHP2 signaling
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
TCR signaling in naïve CD4+ T cells
NCI :
VEGFR1 specific signals
Reactome :
Activated TLR4 signalling
Reactome :
Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
Reactome :
Adaptive Immune System
Reactome :
Axon guidance
Reactome :
Cell surface interactions at the vascular wall
Reactome :
Cell-Cell communication
Reactome :
Constitutive PI3K/AKT Signaling in Cancer
Reactome :
Costimulation by the CD28 family
Reactome :
CTLA4 inhibitory signaling
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
Downstream signaling of activated FGFR
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FRS2-mediated cascade
Reactome :
GAB1 signalosome
Reactome :
Hemostasis
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interferon alpha/beta signaling
Reactome :
Interferon gamma signaling
Reactome :
Interferon Signaling
Reactome :
Interleukin-3, 5 and GM-CSF signaling
Reactome :
Interleukin-6 signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
MyD88-independent cascade
Reactome :
Negative regulation of FGFR signaling
Reactome :
Netrin mediated repulsion signals
Reactome :
Netrin-1 signaling
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Nuclear signaling by ERBB4
Reactome :
PD-1 signaling
Reactome :
PECAM1 interactions
Reactome :
PI-3K cascade
Reactome :
PI3K Cascade
Reactome :
PI3K events in ERBB2 signaling
Reactome :
PI3K events in ERBB4 signaling
Reactome :
PI3K/AKT activation
Reactome :
PI3K/AKT Signaling in Cancer
Reactome :
PIP3 activates AKT signaling
Reactome :
Platelet homeostasis
Reactome :
Platelet sensitization by LDL
Reactome :
Prolactin receptor signaling
Reactome :
Regulation of IFNA signaling
Reactome :
Regulation of IFNG signaling
Reactome :
Role of LAT2/NTAL/LAB on calcium mobilization
Reactome :
Signal regulatory protein (SIRP) family interactions
Reactome :
Signal Transduction
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Spry regulation of FGF signaling
Reactome :
Tie2 Signaling
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Heart Development
WikiPathway :
IL-1 signaling pathway
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
IL-4 signaling pathway
WikiPathway :
IL-5 signaling pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
Proteins and DNA Sequences in Cardicac Structures
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
Type II interferon signaling (IFNG)
Protein
HPRD :
01470
Organism
Species Specific ID:
5781
Genome
Chromosome :
12
Map Location :
12q24
Ensembl :
ENSG00000179295