Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments:
None
Publications:
PMID:20394823 Title: High-throughput expression and purification of membrane proteins. PMID:24510647 Title: High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli.
Authors:
New York Consortium on Membrane Protein Structure
PSI
New York Structural Biology Center
EvNO00091443
Price:
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AVAILABLE
No restriction
Special MTA:
PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DB3.1 T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments:
None
Size (bp):
6984
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
New York Consortium on Membrane Protein Structure
New York Structural Biology Center