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Detailed Vector Information: pNYCOMPS-LIC-FH10T+ (N term)

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pNYCOMPS-LIC-FH10T+ (N term)              
Source: None
Description: Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments: None
Publications: PMID: 20394823
Title: High-throughput expression and purification of membrane proteins.
PMID: 24510647
Title: High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli.
Authors: PSI
New York Consortium on Membrane Protein Structure
New York Structural Biology Center
EvNO00091443

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DB3.1 T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

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Vector Name: pNYCOMPS-LIC-FH10T+ (N term)

pNYCOMPS-LIC-FH10TNterm in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments: None
Size (bp): 6984
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: New York Consortium on Membrane Protein Structure
New York Structural Biology Center
Publications: PMID: 20394823
Title: High-throughput expression and purification of membrane proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1583 2265
death cassette ccdB ccdB death cassette (removed in plasmids containing an insert) 5393 5602
promoter T7 T7 promoter 5146 5165
protease cleavage site TEV TEV protease cleavage site 5290 5310
repressor protein gene LacI Lac inhibitor 3677 4693
ribosome binding site RBS Ribosome Binding Site 5225 5230
selectable marker KanR kanamycin resistance 725 1537
selectable marker CmR Chloramphenicol resistance gene 6040 6696
tag His 10xHis tag; N terminal 5260 5289
tag Flag Flag epitope tag; N terminal 5236 5259
trxn regulatory element lac operator lac operator 5165 5187
trxn termination sequence T7 term T7 terminator 91 137

 

Species Specific ID: None
Target GenBank: None