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EIF3S3 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EIF3H
Gene Name:  eukaryotic translation initiation factor 3, subunit H
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH007219.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00000928

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1059nts         Open reading frame : 1 to 1059
Reference Sequence Alignment

ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1064
Start on reference sequence 6
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EIF3H
Symbol Nomenclature : EIF3H
SYNONYM : EIF3S3; eIF3-gamma; eIF3-p40
Designation : eIF-3-gamma|eIF3 p40 subunit|eukaryotic translation initiation factor 3 subunit 3|eukaryotic translation initiation factor 3 subunit H|eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD)|eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD)|eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa
Full Nomenclature : eukaryotic translation initiation factor 3, subunit H
GENEID : 8667
GI : 61357535
GenBank Accession : AY888459
HGNC : 3273
MIM : 603912
Target GenBank: NM_003756

 

Reference Sequence Alignment

  

HsCD00000928      ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
NM_003756.2       ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
                  ************************************************************

HsCD00000928      GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
NM_003756.2       GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
                  ************************************************************

HsCD00000928      ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
NM_003756.2       ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
                  ************************************************************

HsCD00000928      GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
NM_003756.2       GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
                  ************************************************************

HsCD00000928      TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
NM_003756.2       TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
                  ************************************************************

HsCD00000928      ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
NM_003756.2       ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
                  ************************************************************

HsCD00000928      CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
NM_003756.2       CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
                  ************************************************************

HsCD00000928      CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
NM_003756.2       CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
                  ************************************************************

HsCD00000928      TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
NM_003756.2       TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
                  ************************************************************

HsCD00000928      GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
NM_003756.2       GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
                  ************************************************************

HsCD00000928      GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
NM_003756.2       GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
                  ************************************************************

HsCD00000928      AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
NM_003756.2       AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
                  ************************************************************

HsCD00000928      AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
NM_003756.2       AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
                  ************************************************************

HsCD00000928      AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
NM_003756.2       AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
                  ************************************************************

HsCD00000928      CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
NM_003756.2       CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
                  ************************************************************

HsCD00000928      CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
NM_003756.2       CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
                  ************************************************************

HsCD00000928      GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
NM_003756.2       GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
                  ************************************************************

HsCD00000928      CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAACTAG
NM_003756.2       CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAACTAA
                  **************************************.

Pathways

Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Eukaryotic Translation Initiation
Reactome : Formation of a pool of free 40S subunits
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of proteins
Reactome : Ribosomal scanning and start codon recognition
Reactome : Translation
Reactome : Translation initiation complex formation
WikiPathway : Translation Factors

Protein

SMART domain : JAB_MPN : JAB/MPN domain
UniProt : O15372
UniProt : Q6IB98
HPRD : 04885

Clone

Cloning Information : 7219
HIP Master Clone ID : 6777
Original Clone ID : FLH007219.01X

Organism


TAX_ID : 9606
Species Specific ID: 8667

Genome

Chromosome : 8
Map Location : 8q24.11

Reagents

Labome : EIF3H-antibody