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ACTR2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR2
Gene Name:  ARP2 actin-related protein 2 homolog (yeast)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH007737.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00000933

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1185nts        
Open reading frame : 1 to 1185
Reference Sequence Alignment

ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACCGGGTTTGTGAAGTGT
GGATATGCAGGCTCTAACTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATT
ATCAGATCAACCACCAAAGTGGGAAACATTGAAATCAAGGATCTTATGGTTGGTGATGAG
GCAAGTGAATTACGATCAATGTTAGAAGTTAACTACCCTATGGAAAATGGCATAGTACGA
AATTGGGATGACATGAAACACCTGTGGGACTACACATTTGGACCAGAGAAACTTAATATA
GATACCAGAAATTGTAAAATCTTACTCACAGAACCTCCTATGAACCCAACCAAAAACAGA
GAGAAGATTGTAGAGGTAATGTTTGAAACTTACCAGTTTTCCGGTGTATATGTAGCCATC
CAGGCAGTTCTGACTTTGTACGCTCAAGGTTTATTGACTGGTGTAGTGGTAGACTCTGGA
GATGGTGTGACTCACATTTGCCCAGTATATGAAGGCTTTTCTCTCCCTCATCTTACCAGG
AGACTGGATATTGCTGGGAGGGATATAACTAGATATCTTATCAAGCTACTTCTGTTGCGA
GGATACGCCTTCAACCACTCTGCTGATTTTGAAACGGTTCGCATGATTAAAGAAAAACTG
TGTTACGTGGGATATAATATTGAGCAAGAGCAGAAACTGGCCTTAGAAACCACAGTATTA
GTTGAATCTTATACACTCCCAGATGGACGTATCATCAAAGTTGGGGGAGAGAGATTTGAA
GCACCAGAAGCTTTATTTCAGCCTCACTTGATCAATGTTGAAGGAGTTGGTGTTGCTGAA
TTGCTTTTTAACACAATTCAGGCAGCTGACATTGATACCAGATCTGAATTCTACAAACAC
ATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCATCACGGTTGGAACGAGAA
CTTAAACAGCTTTACTTAGAACGAGTTTTGAAGGGTGATGTGGAAAAACTTTCTAAATTT
AAGATCCGCATTGAAGACCCACCCCGCAGAAAGCACATGGTATTCCTGGGTGGTGCAGTT
CTAGCGGATATCATGAAAGACAAAGACAACTTTTGGATGACCCGACAAGAGTACCAAGAA
AAGGGTGTCCGTGTGCTAGAGAAACTTGGTGTGACTGTTCGATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1259
Start on reference sequence 75
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 9 42
recombination site loxP site loxP recombination site 1015 1048
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR2
Symbol Nomenclature : ACTR2
SYNONYM : ARP2
Designation : actin-like protein 2|actin-related protein 2
Full Nomenclature : ARP2 actin-related protein 2 homolog (yeast)
GENEID : 10097
GI : 61367660
GenBank Accession : AY891099
HGNC : 169
MIM : 604221
Vega : OTTHUMG00000129540
Target GenBank: NM_005722

 

Reference Sequence Alignment

  

HsCD00000933        ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACCGGGTTTGTGAAGTGT
NM_001005386.2      ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACCGGGTTTGTGAAGTGT
NM_005722.3         ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACCGGGTTTGTGAAGTGT
                    ************************************************************

HsCD00000933        GGATATGCAGGCTCTAACTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATT
NM_001005386.2      GGATATGCAGGCTCTAACTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATT
NM_005722.3         GGATATGCAGGCTCTAACTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATT
                    ************************************************************

HsCD00000933        ATCAGATCAACCACCAAAGTGGGAAACATTGAAATCAAG---------------GATCTT
NM_001005386.2      ATCAGATCAACCACCAAAGTGGGAAACATTGAAATCAAGAATAACAAAAAGATGGATCTT
NM_005722.3         ATCAGATCAACCACCAAAGTGGGAAACATTGAAATCAAG---------------GATCTT
                    ***************************************               ******

HsCD00000933        ATGGTTGGTGATGAGGCAAGTGAATTACGATCAATGTTAGAAGTTAACTACCCTATGGAA
NM_001005386.2      ATGGTTGGTGATGAGGCAAGTGAATTACGATCAATGTTAGAAGTTAACTACCCTATGGAA
NM_005722.3         ATGGTTGGTGATGAGGCAAGTGAATTACGATCAATGTTAGAAGTTAACTACCCTATGGAA
                    ************************************************************

HsCD00000933        AATGGCATAGTACGAAATTGGGATGACATGAAACACCTGTGGGACTACACATTTGGACCA
NM_001005386.2      AATGGCATAGTACGAAATTGGGATGACATGAAACACCTGTGGGACTACACATTTGGACCA
NM_005722.3         AATGGCATAGTACGAAATTGGGATGACATGAAACACCTGTGGGACTACACATTTGGACCA
                    ************************************************************

HsCD00000933        GAGAAACTTAATATAGATACCAGAAATTGTAAAATCTTACTCACAGAACCTCCTATGAAC
NM_001005386.2      GAGAAACTTAATATAGATACCAGAAATTGTAAAATCTTACTCACAGAACCTCCTATGAAC
NM_005722.3         GAGAAACTTAATATAGATACCAGAAATTGTAAAATCTTACTCACAGAACCTCCTATGAAC
                    ************************************************************

HsCD00000933        CCAACCAAAAACAGAGAGAAGATTGTAGAGGTAATGTTTGAAACTTACCAGTTTTCCGGT
NM_001005386.2      CCAACCAAAAACAGAGAGAAGATTGTAGAGGTAATGTTTGAAACTTACCAGTTTTCCGGT
NM_005722.3         CCAACCAAAAACAGAGAGAAGATTGTAGAGGTAATGTTTGAAACTTACCAGTTTTCCGGT
                    ************************************************************

HsCD00000933        GTATATGTAGCCATCCAGGCAGTTCTGACTTTGTACGCTCAAGGTTTATTGACTGGTGTA
NM_001005386.2      GTATATGTAGCCATCCAGGCAGTTCTGACTTTGTACGCTCAAGGTTTATTGACTGGTGTA
NM_005722.3         GTATATGTAGCCATCCAGGCAGTTCTGACTTTGTACGCTCAAGGTTTATTGACTGGTGTA
                    ************************************************************

HsCD00000933        GTGGTAGACTCTGGAGATGGTGTGACTCACATTTGCCCAGTATATGAAGGCTTTTCTCTC
NM_001005386.2      GTGGTAGACTCTGGAGATGGTGTGACTCACATTTGCCCAGTATATGAAGGCTTTTCTCTC
NM_005722.3         GTGGTAGACTCTGGAGATGGTGTGACTCACATTTGCCCAGTATATGAAGGCTTTTCTCTC
                    ************************************************************

HsCD00000933        CCTCATCTTACCAGGAGACTGGATATTGCTGGGAGGGATATAACTAGATATCTTATCAAG
NM_001005386.2      CCTCATCTTACCAGGAGACTGGATATTGCTGGGAGGGATATAACTAGATATCTTATCAAG
NM_005722.3         CCTCATCTTACCAGGAGACTGGATATTGCTGGGAGGGATATAACTAGATATCTTATCAAG
                    ************************************************************

HsCD00000933        CTACTTCTGTTGCGAGGATACGCCTTCAACCACTCTGCTGATTTTGAAACGGTTCGCATG
NM_001005386.2      CTACTTCTGTTGCGAGGATACGCCTTCAACCACTCTGCTGATTTTGAAACGGTTCGCATG
NM_005722.3         CTACTTCTGTTGCGAGGATACGCCTTCAACCACTCTGCTGATTTTGAAACGGTTCGCATG
                    ************************************************************

HsCD00000933        ATTAAAGAAAAACTGTGTTACGTGGGATATAATATTGAGCAAGAGCAGAAACTGGCCTTA
NM_001005386.2      ATTAAAGAAAAACTGTGTTACGTGGGATATAATATTGAGCAAGAGCAGAAACTGGCCTTA
NM_005722.3         ATTAAAGAAAAACTGTGTTACGTGGGATATAATATTGAGCAAGAGCAGAAACTGGCCTTA
                    ************************************************************

HsCD00000933        GAAACCACAGTATTAGTTGAATCTTATACACTCCCAGATGGACGTATCATCAAAGTTGGG
NM_001005386.2      GAAACCACAGTATTAGTTGAATCTTATACACTCCCAGATGGACGTATCATCAAAGTTGGG
NM_005722.3         GAAACCACAGTATTAGTTGAATCTTATACACTCCCAGATGGACGTATCATCAAAGTTGGG
                    ************************************************************

HsCD00000933        GGAGAGAGATTTGAAGCACCAGAAGCTTTATTTCAGCCTCACTTGATCAATGTTGAAGGA
NM_001005386.2      GGAGAGAGATTTGAAGCACCAGAAGCTTTATTTCAGCCTCACTTGATCAATGTTGAAGGA
NM_005722.3         GGAGAGAGATTTGAAGCACCAGAAGCTTTATTTCAGCCTCACTTGATCAATGTTGAAGGA
                    ************************************************************

HsCD00000933        GTTGGTGTTGCTGAATTGCTTTTTAACACAATTCAGGCAGCTGACATTGATACCAGATCT
NM_001005386.2      GTTGGTGTTGCTGAATTGCTTTTTAACACAATTCAGGCAGCTGACATTGATACCAGATCT
NM_005722.3         GTTGGTGTTGCTGAATTGCTTTTTAACACAATTCAGGCAGCTGACATTGATACCAGATCT
                    ************************************************************

HsCD00000933        GAATTCTACAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCATCA
NM_001005386.2      GAATTCTACAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCATCA
NM_005722.3         GAATTCTACAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCATCA
                    ************************************************************

HsCD00000933        CGGTTGGAACGAGAACTTAAACAGCTTTACTTAGAACGAGTTTTGAAGGGTGATGTGGAA
NM_001005386.2      CGGTTGGAACGAGAACTTAAACAGCTTTACTTAGAACGAGTTTTGAAGGGTGATGTGGAA
NM_005722.3         CGGTTGGAACGAGAACTTAAACAGCTTTACTTAGAACGAGTTTTGAAGGGTGATGTGGAA
                    ************************************************************

HsCD00000933        AAACTTTCTAAATTTAAGATCCGCATTGAAGACCCACCCCGCAGAAAGCACATGGTATTC
NM_001005386.2      AAACTTTCTAAATTTAAGATCCGCATTGAAGACCCACCCCGCAGAAAGCACATGGTATTC
NM_005722.3         AAACTTTCTAAATTTAAGATCCGCATTGAAGACCCACCCCGCAGAAAGCACATGGTATTC
                    ************************************************************

HsCD00000933        CTGGGTGGTGCAGTTCTAGCGGATATCATGAAAGACAAAGACAACTTTTGGATGACCCGA
NM_001005386.2      CTGGGTGGTGCAGTTCTAGCGGATATCATGAAAGACAAAGACAACTTTTGGATGACCCGA
NM_005722.3         CTGGGTGGTGCAGTTCTAGCGGATATCATGAAAGACAAAGACAACTTTTGGATGACCCGA
                    ************************************************************

HsCD00000933        CAAGAGTACCAAGAAAAGGGTGTCCGTGTGCTAGAGAAACTTGGTGTGACTGTTCGATTG
NM_001005386.2      CAAGAGTACCAAGAAAAGGGTGTCCGTGTGCTAGAGAAACTTGGTGTGACTGTTCGATAA
NM_005722.3         CAAGAGTACCAAGAAAAGGGTGTCCGTGTGCTAGAGAAACTTGGTGTGACTGTTCGATAA
                    **********************************************************:.

Pathways

NCI : CDC42 signaling events
NCI : ErbB1 downstream signaling
NCI : PDGFR-beta signaling pathway
NCI : RAC1 signaling pathway
Panther : Alzheimer disease-presenilin pathway
Panther : Cadherin signaling pathway
Panther : Huntington disease
Reactome : Fcgamma receptor (FCGR) dependent phagocytosis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Regulation of actin dynamics for phagocytic cup formation

Protein

SMART domain : ACTIN : Actin
UniProt : E9PF41
UniProt : P61160
HPRD : 05023

Clone

Cloning Information : 7737
HIP Master Clone ID : 7295
Original Clone ID : FLH007737.01L

Organism


TAX_ID : 9606
Species Specific ID: 10097

Genome

Chromosome : 2
Map Location : 2p14
Ensembl : ENSG00000138071

Reagents

Labome : ACTR2-antibody