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EGR2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EGR2
Gene Name:  early growth response 2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH007869.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00000954

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1431nts         Open reading frame : 1 to 1431
Reference Sequence Alignment

ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAG
CTGTCTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGTGACCATCTTTCCC
AATGCCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATC
AACATTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCT
CCCGTCTCTGCACCTAGAAACCAGACCTTCACTTACATGGGCAAGTTCTCCATTGACCCT
CAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATAATCAATATTGTGAGTGCAGGCATC
TTGCAAGGGGTCACTTCCCCAGCTTCAACCACAGCCTCATCCAGCGTCACCTCTGCCTCC
CCCAACCCACTGGCCACAGGACCCCTGGGTGTGTGCACCATGTCCCAGACCCAGCCTGAC
CTGGACCACCTGTACTCTCCGCCACCGCCTCCTCCTCCTTATTCTGGCTGTGCAGGAGAC
CTCTACCAGGACCCTTCTGCGTTCCTGTCAGCAGCCACCACCTCCACCTCTTCCTCTCTG
GCCTACCCACCACCTCCTTCCTATCCATCCCCCAAGCCAGCCACGGACCCAGGTCTCTTC
CCAATGATCCCAGACTATCCTGGATTCTTTCCATCTCAGTGCCAGAGAGACCTACATGGT
ACAGCTGGCCCAGACCGTAAGCCCTTTCCCTGCCCACTGGACACCCTGCGGGTGCCCCCT
CCACTCACTCCACTCTCTACAATCCGTAACTTTACCCTGGGGGGCCCCAGTGCTGGGGTG
ACCGGACCAGGGGCCAGTGGAGGCAGCGAGGGACCCCGGCTGCCTGGTAGCAGCTCAGCA
GCAGCAGCAGCCGCCGCCGCCGCCGCCTATAACCCACACCACCTGCCACTGCGGCCCATT
CTGAGGCCTCGCAAGTACCCCAACAGACCCAGCAAGACGCCGGTGCACGAGAGGCCCTAC
CCGTGCCCAGCAGAAGGCTGCGACCGGCGGTTCTCCCGCTCTGACGAGCTGACACGGCAC
ATCCGAATCCACACTGGGCATAAGCCCTTCCAGTGTCGGATCTGCATGCGCAACTTCAGC
CGCAGTGACCACCTCACCACCCATATCCGCACCCACACCGGTGAGAAGCCCTTCGCCTGT
GACTACTGTGGCCGAAAGTTTGCCCGGAGTGATGAGAGGAAGCGCCACACCAAGATCCAC
CTGAGACAGAAAGAGCGGAAAAGCAGTGCCCCCTCTGCATCGGTGCCAGCCCCCTCTACA
GCCTCCTGCTCTGGGGGCGTGCAGCCTGGGGGTACCCTGTGCAGCAGTAACAGCAGCAGT
CTTGGCGGAGGGCCGCTCGCCCCTTGCTCCTCTCGGACCCGGACACCTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1769
Start on reference sequence 339
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EGR2
Symbol Nomenclature : EGR2
SYNONYM : AT591; CMT1D; CMT4E; KROX20
Designation : E3 SUMO-protein ligase EGR2|KROX-20, Drosophila, homolog (early growth response-2)|early growth response protein 2|zinc finger protein Krox-20
Full Nomenclature : early growth response 2
GENEID : 1959
GI : 61367757
GenBank Accession : AY891114
HGNC : 3239
MIM : 129010
Vega : OTTHUMG00000018308
Target GenBank: NM_000399

 

Reference Sequence Alignment

  

HsCD00000954        ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAG
NM_001136178.1      ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAG
NM_001136177.1      ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAG
NM_000399.3         ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAG
                    ************************************************************

HsCD00000954        CTGTCTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGTGACCATCTTTCCC
NM_001136178.1      CTGTCTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGTGACCATCTTTCCC
NM_001136177.1      CTGTCTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGTGACCATCTTTCCC
NM_000399.3         CTGTCTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGTGACCATCTTTCCC
                    ************************************************************

HsCD00000954        AATGCCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATC
NM_001136178.1      AATGCCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATC
NM_001136177.1      AATGCCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATC
NM_000399.3         AATGCCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATC
                    ************************************************************

HsCD00000954        AACATTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCT
NM_001136178.1      AACATTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCT
NM_001136177.1      AACATTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCT
NM_000399.3         AACATTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCT
                    ************************************************************

HsCD00000954        CCCGTCTCTGCACCTAGAAACCAGACCTTCACTTACATGGGCAAGTTCTCCATTGACCCT
NM_001136178.1      CCCGTCTCTGCACCTAGAAACCAGACCTTCACTTACATGGGCAAGTTCTCCATTGACCCT
NM_001136177.1      CCCGTCTCTGCACCTAGAAACCAGACCTTCACTTACATGGGCAAGTTCTCCATTGACCCT
NM_000399.3         CCCGTCTCTGCACCTAGAAACCAGACCTTCACTTACATGGGCAAGTTCTCCATTGACCCT
                    ************************************************************

HsCD00000954        CAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATAATCAATATTGTGAGTGCAGGCATC
NM_001136178.1      CAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATAATCAATATTGTGAGTGCAGGCATC
NM_001136177.1      CAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATAATCAATATTGTGAGTGCAGGCATC
NM_000399.3         CAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATAATCAATATTGTGAGTGCAGGCATC
                    ************************************************************

HsCD00000954        TTGCAAGGGGTCACTTCCCCAGCTTCAACCACAGCCTCATCCAGCGTCACCTCTGCCTCC
NM_001136178.1      TTGCAAGGGGTCACTTCCCCAGCTTCAACCACAGCCTCATCCAGCGTCACCTCTGCCTCC
NM_001136177.1      TTGCAAGGGGTCACTTCCCCAGCTTCAACCACAGCCTCATCCAGCGTCACCTCTGCCTCC
NM_000399.3         TTGCAAGGGGTCACTTCCCCAGCTTCAACCACAGCCTCATCCAGCGTCACCTCTGCCTCC
                    ************************************************************

HsCD00000954        CCCAACCCACTGGCCACAGGACCCCTGGGTGTGTGCACCATGTCCCAGACCCAGCCTGAC
NM_001136178.1      CCCAACCCACTGGCCACAGGACCCCTGGGTGTGTGCACCATGTCCCAGACCCAGCCTGAC
NM_001136177.1      CCCAACCCACTGGCCACAGGACCCCTGGGTGTGTGCACCATGTCCCAGACCCAGCCTGAC
NM_000399.3         CCCAACCCACTGGCCACAGGACCCCTGGGTGTGTGCACCATGTCCCAGACCCAGCCTGAC
                    ************************************************************

HsCD00000954        CTGGACCACCTGTACTCTCCGCCACCGCCTCCTCCTCCTTATTCTGGCTGTGCAGGAGAC
NM_001136178.1      CTGGACCACCTGTACTCTCCGCCACCGCCTCCTCCTCCTTATTCTGGCTGTGCAGGAGAC
NM_001136177.1      CTGGACCACCTGTACTCTCCGCCACCGCCTCCTCCTCCTTATTCTGGCTGTGCAGGAGAC
NM_000399.3         CTGGACCACCTGTACTCTCCGCCACCGCCTCCTCCTCCTTATTCTGGCTGTGCAGGAGAC
                    ************************************************************

HsCD00000954        CTCTACCAGGACCCTTCTGCGTTCCTGTCAGCAGCCACCACCTCCACCTCTTCCTCTCTG
NM_001136178.1      CTCTACCAGGACCCTTCTGCGTTCCTGTCAGCAGCCACCACCTCCACCTCTTCCTCTCTG
NM_001136177.1      CTCTACCAGGACCCTTCTGCGTTCCTGTCAGCAGCCACCACCTCCACCTCTTCCTCTCTG
NM_000399.3         CTCTACCAGGACCCTTCTGCGTTCCTGTCAGCAGCCACCACCTCCACCTCTTCCTCTCTG
                    ************************************************************

HsCD00000954        GCCTACCCACCACCTCCTTCCTATCCATCCCCCAAGCCAGCCACGGACCCAGGTCTCTTC
NM_001136178.1      GCCTACCCACCACCTCCTTCCTATCCATCCCCCAAGCCAGCCACGGACCCAGGTCTCTTC
NM_001136177.1      GCCTACCCACCACCTCCTTCCTATCCATCCCCCAAGCCAGCCACGGACCCAGGTCTCTTC
NM_000399.3         GCCTACCCACCACCTCCTTCCTATCCATCCCCCAAGCCAGCCACGGACCCAGGTCTCTTC
                    ************************************************************

HsCD00000954        CCAATGATCCCAGACTATCCTGGATTCTTTCCATCTCAGTGCCAGAGAGACCTACATGGT
NM_001136178.1      CCAATGATCCCAGACTATCCTGGATTCTTTCCATCTCAGTGCCAGAGAGACCTACATGGT
NM_001136177.1      CCAATGATCCCAGACTATCCTGGATTCTTTCCATCTCAGTGCCAGAGAGACCTACATGGT
NM_000399.3         CCAATGATCCCAGACTATCCTGGATTCTTTCCATCTCAGTGCCAGAGAGACCTACATGGT
                    ************************************************************

HsCD00000954        ACAGCTGGCCCAGACCGTAAGCCCTTTCCCTGCCCACTGGACACCCTGCGGGTGCCCCCT
NM_001136178.1      ACAGCTGGCCCAGACCGTAAGCCCTTTCCCTGCCCACTGGACACCCTGCGGGTGCCCCCT
NM_001136177.1      ACAGCTGGCCCAGACCGTAAGCCCTTTCCCTGCCCACTGGACACCCTGCGGGTGCCCCCT
NM_000399.3         ACAGCTGGCCCAGACCGTAAGCCCTTTCCCTGCCCACTGGACACCCTGCGGGTGCCCCCT
                    ************************************************************

HsCD00000954        CCACTCACTCCACTCTCTACAATCCGTAACTTTACCCTGGGGGGCCCCAGTGCTGGGGTG
NM_001136178.1      CCACTCACTCCACTCTCTACAATCCGTAACTTTACCCTGGGGGGCCCCAGTGCTGGGGTG
NM_001136177.1      CCACTCACTCCACTCTCTACAATCCGTAACTTTACCCTGGGGGGCCCCAGTGCTGGGGTG
NM_000399.3         CCACTCACTCCACTCTCTACAATCCGTAACTTTACCCTGGGGGGCCCCAGTGCTGGGGTG
                    ************************************************************

HsCD00000954        ACCGGACCAGGGGCCAGTGGAGGCAGCGAGGGACCCCGGCTGCCTGGTAGCAGCTCAGCA
NM_001136178.1      ACCGGACCAGGGGCCAGTGGAGGCAGCGAGGGACCCCGGCTGCCTGGTAGCAGCTCAGCA
NM_001136177.1      ACCGGACCAGGGGCCAGTGGAGGCAGCGAGGGACCCCGGCTGCCTGGTAGCAGCTCAGCA
NM_000399.3         ACCGGACCAGGGGCCAGTGGAGGCAGCGAGGGACCCCGGCTGCCTGGTAGCAGCTCAGCA
                    ************************************************************

HsCD00000954        GCAGCAGCAGCCGCCGCCGCCGCCGCCTATAACCCACACCACCTGCCACTGCGGCCCATT
NM_001136178.1      GCAGCAGCAGCCGCCGCCGCCGCCGCCTATAACCCACACCACCTGCCACTGCGGCCCATT
NM_001136177.1      GCAGCAGCAGCCGCCGCCGCCGCCGCCTATAACCCACACCACCTGCCACTGCGGCCCATT
NM_000399.3         GCAGCAGCAGCCGCCGCCGCCGCCGCCTATAACCCACACCACCTGCCACTGCGGCCCATT
                    ************************************************************

HsCD00000954        CTGAGGCCTCGCAAGTACCCCAACAGACCCAGCAAGACGCCGGTGCACGAGAGGCCCTAC
NM_001136178.1      CTGAGGCCTCGCAAGTACCCCAACAGACCCAGCAAGACGCCGGTGCACGAGAGGCCCTAC
NM_001136177.1      CTGAGGCCTCGCAAGTACCCCAACAGACCCAGCAAGACGCCGGTGCACGAGAGGCCCTAC
NM_000399.3         CTGAGGCCTCGCAAGTACCCCAACAGACCCAGCAAGACGCCGGTGCACGAGAGGCCCTAC
                    ************************************************************

HsCD00000954        CCGTGCCCAGCAGAAGGCTGCGACCGGCGGTTCTCCCGCTCTGACGAGCTGACACGGCAC
NM_001136178.1      CCGTGCCCAGCAGAAGGCTGCGACCGGCGGTTCTCCCGCTCTGACGAGCTGACACGGCAC
NM_001136177.1      CCGTGCCCAGCAGAAGGCTGCGACCGGCGGTTCTCCCGCTCTGACGAGCTGACACGGCAC
NM_000399.3         CCGTGCCCAGCAGAAGGCTGCGACCGGCGGTTCTCCCGCTCTGACGAGCTGACACGGCAC
                    ************************************************************

HsCD00000954        ATCCGAATCCACACTGGGCATAAGCCCTTCCAGTGTCGGATCTGCATGCGCAACTTCAGC
NM_001136178.1      ATCCGAATCCACACTGGGCATAAGCCCTTCCAGTGTCGGATCTGCATGCGCAACTTCAGC
NM_001136177.1      ATCCGAATCCACACTGGGCATAAGCCCTTCCAGTGTCGGATCTGCATGCGCAACTTCAGC
NM_000399.3         ATCCGAATCCACACTGGGCATAAGCCCTTCCAGTGTCGGATCTGCATGCGCAACTTCAGC
                    ************************************************************

HsCD00000954        CGCAGTGACCACCTCACCACCCATATCCGCACCCACACCGGTGAGAAGCCCTTCGCCTGT
NM_001136178.1      CGCAGTGACCACCTCACCACCCATATCCGCACCCACACCGGTGAGAAGCCCTTCGCCTGT
NM_001136177.1      CGCAGTGACCACCTCACCACCCATATCCGCACCCACACCGGTGAGAAGCCCTTCGCCTGT
NM_000399.3         CGCAGTGACCACCTCACCACCCATATCCGCACCCACACCGGTGAGAAGCCCTTCGCCTGT
                    ************************************************************

HsCD00000954        GACTACTGTGGCCGAAAGTTTGCCCGGAGTGATGAGAGGAAGCGCCACACCAAGATCCAC
NM_001136178.1      GACTACTGTGGCCGAAAGTTTGCCCGGAGTGATGAGAGGAAGCGCCACACCAAGATCCAC
NM_001136177.1      GACTACTGTGGCCGAAAGTTTGCCCGGAGTGATGAGAGGAAGCGCCACACCAAGATCCAC
NM_000399.3         GACTACTGTGGCCGAAAGTTTGCCCGGAGTGATGAGAGGAAGCGCCACACCAAGATCCAC
                    ************************************************************

HsCD00000954        CTGAGACAGAAAGAGCGGAAAAGCAGTGCCCCCTCTGCATCGGTGCCAGCCCCCTCTACA
NM_001136178.1      CTGAGACAGAAAGAGCGGAAAAGCAGTGCCCCCTCTGCATCGGTGCCAGCCCCCTCTACA
NM_001136177.1      CTGAGACAGAAAGAGCGGAAAAGCAGTGCCCCCTCTGCATCGGTGCCAGCCCCCTCTACA
NM_000399.3         CTGAGACAGAAAGAGCGGAAAAGCAGTGCCCCCTCTGCATCGGTGCCAGCCCCCTCTACA
                    ************************************************************

HsCD00000954        GCCTCCTGCTCTGGGGGCGTGCAGCCTGGGGGTACCCTGTGCAGCAGTAACAGCAGCAGT
NM_001136178.1      GCCTCCTGCTCTGGGGGCGTGCAGCCTGGGGGTACCCTGTGCAGCAGTAACAGCAGCAGT
NM_001136177.1      GCCTCCTGCTCTGGGGGCGTGCAGCCTGGGGGTACCCTGTGCAGCAGTAACAGCAGCAGT
NM_000399.3         GCCTCCTGCTCTGGGGGCGTGCAGCCTGGGGGTACCCTGTGCAGCAGTAACAGCAGCAGT
                    ************************************************************

HsCD00000954        CTTGGCGGAGGGCCGCTCGCCCCTTGCTCCTCTCGGACCCGGACACCTTTG
NM_001136178.1      CTTGGCGGAGGGCCGCTCGCCCCTTGCTCCTCTCGGACCCGGACACCTTGA
NM_001136177.1      CTTGGCGGAGGGCCGCTCGCCCCTTGCTCCTCTCGGACCCGGACACCTTGA
NM_000399.3         CTTGGCGGAGGGCCGCTCGCCCCTTGCTCCTCTCGGACCCGGACACCTTGA
                    ************************************************* .

Pathways

NCI : Calcineurin-regulated NFAT-dependent transcription in lymphocytes
NCI : IL4-mediated signaling events
NCI : Validated transcriptional targets of TAp63 isoforms
Reactome : Developmental Biology
Reactome : Transcriptional regulation of white adipocyte differentiation
WikiPathway : Adipogenesis

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : P11161
HPRD : 00551

Clone

Cloning Information : 7869
HIP Master Clone ID : 7427
Original Clone ID : FLH007869.01L

Organism


TAX_ID : 9606
Species Specific ID: 1959

Genome

Chromosome : 10
Map Location : 10q21.1
Ensembl : ENSG00000122877

Reagents

Labome : EGR2-antibody