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VTH2 (Saccharomyces cerevisiae) in pBY011 (yeast expression vector)

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Explanation of Terms


Gene: Gene Symbol:  VTH2
Gene Name:  vps ten homolog
Sequence             
Original Clone ID: FLH261919.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pBY011               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
ScCD00096258

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4650nts         Open reading frame : 1 to 4650

ATGGCATTGTTTCGTGCCCTGTATATTATTTGGGTATTCCTACTCATTCCACTGTCCAAT
GCAGAAGAATTCACCCCCAAGGTTACCAGAACTTTGTCAAGATATGTATTCGATATAGTA
AACTTTGATGATTCAAACACTTTGATCAGAGCAGAAGAAGACTCTGTCGAGATAAGTTTT
GATGCTGGAGAAAATTGGAAAACAATAGATGAGATTGAAGAGCCTATCGAGTCATTTGTT
GTTGATCCCTTCCGTGGGCATGATAGAGCTTTTGCTTTCGTGAAAACGGCACCAAAATTT
TACGTCACGGATGATCAAGGTAAATCGTGGAGGCCTTTGACTATACCCATTTCTGAGAAA
GCATCGAATTATTTTTGCGACGTAACTACTCACCCTATAAAAAAGAAGCATCTTATTATT
CGTTGTGATTTGTTGACAATAAAAAACTCAGGCTTAATGTATGTTGGAAGAGAGATTTAC
ACAACTAATGATGGAGTATCCTTTTCCCAAGTTAAACCTTCTTTTGGAAAAATTGATGGT
CATATTAGCACGGCACGCTGTGACTTTATTAAATCTAGTGAGGATTCTGATCTGGGCGGT
AATGATGCTTCGATACTCTGTCTTTTCCGAAACACTGAGTACATTGAAAGTACAGGCTCA
ACTATTGACAAGTCTGAATTGATTTTAAGTGCCGATGGAGGTGAAACATTCAAAGAATTA
GTCCAGTTCAAAGATAAGGTTGTTAGTCGATACGAGATACTTAAGCATCATGTGATCGTT
TTGACACAAGATGATATGTATAATGAAATGTCATCAACAAACATTTGGATATCCAATGAT
GTATCCACTTTTCAAGTGGCTCGTACACCTACTAAGATACGGCACGTTAATATGGGACAA
ATTCATGAAGATTCCATCGGAAGAATCGTTTTACCTGTATCTAGAGAAAGAGATGATGAA
GACAGCAACCAACCGGGAGCTGCTGAAGTGTTAATATCAGATTCTGAAGGACTAAAATTT
TTGCCTATAAATTGGATACCAAACAATCAGTTTGGATATATCAATGTAGCTTATCCAGGT
TTCTTAAAAGGAACATTTTTTGGTTCGTTTCATCCCTTCATTGAATATTCTGATAGAAAA
AGGAAATACAGCCGACAGAAAGTAAGAGAGGAAACTAAAGTATCTGTTGATAACGGTCTC
ACATGGACAAACTTGAAAGTTGTTGACCGGGAAAATGTAGATTTATTTGGTTGTGATGTC
ACTAAACCCGAGAGATGTTCGCTTCAGACTCATTTCTATGATCTAAGAAATTTAAATCCC
TCTGCCGGAATCATGATGATATCGGGTATTGTTGGCGACGGCAGTGCATACAATTGGAAA
GAAGAAAAAACTTTCATCTCCAGGGATAGTGGTTTAACATGGAGGTTAGTCCATAATTCC
ACTGGTTTATATACTACTGGTGATCTGGGAAATATTATCATGTATATTCCATATCGTTCG
AATGAAAATGGCGATGTGCCTTCTAAATTTTATTATTCCTTGGATCAAGGTAAGACATGG
GGTGAATATGATCTAATCATGCCTATTTATCCATACAGATTAGTCAGCACAATATCAGAT
GGATCTGGTTCAAAATTTATTCTAACAGGAACGTCCATTACAGAAGATCCAATATTCATT
ACATATTCAATTGATTTTTCAGCGGTATTTGATTATAAATCATGTGAAGAGGGTGATTTT
GAAGATTGGAATCTAGCAGATGGAAAATGTGTTAATGGCGCCAAATACAAGTACAGAAGG
AGGAAACAGGACGCCCAGTGTTTGGTAAAAAAAGCATTCAAAGATTTGAGTTTAGATGAA
ACGCCTTGTAACAGTTGTACTGGATCCGACTACGAATGTTCGTTTGAATTCGTTAGAGAT
GCGAAGGGTGACTGTATACCAGATTATAATCTGATTGCCCTTTCCGATATATGTGACAAA
TCGAAGGGTAAATCTGTGTTAGTAAAGCCACTACAATTAATCAAAGGTGATAAATGTAAA
ACACCTATGAAAATTGAATCCGTAGACATTCCGTGTGACGAAATTCCAAAGGAGGGTTCG
AGTGACAAGGAAATAGTGACTACTGAAAACAAATTCGACTTTGAAATTAAATTTTACCAA
TACTTCGATACAGTTGCCGACGAATCTTTGGTCATGCTGAATTCGATAGGAGACGCCTAT
ATATCCCACGATGGTGGACAAACGATAAAAAGGTTTGACACTGACGGCGAAAAAATTGTC
GAAATCGTGTTTAACCCATACTTCAATTCTTCAGCATATTTGTTTGGCTCTAAAGGTAAC
ATATTCTTAACACACGATAGAGGATACTCTTTCATGATAGCTAAATTACCTGAAGCCAGG
CAATTAGGCATGCCACTAGATTTTAGTGCTAAAGCACAGGATACTTTTATTTACTATGGT
GGTAAGAATTGTGAATCAATTTTAAGTCCAGAATGTCATGCAGTGGCATACCTCACCAAA
GATGGGGGTGAAACTTTTACAGAAATGCTTGATAATGCAATTCATTGTGAGTTTGCAGGA
ACACTATTCAAATATCCGTCAAATGACGATATGGTTATGTGCCAAGTAAAGGAAAAGTTT
TCGCAAACAAGAAGTTTAGTTTCTTCTACTGACTTTTTCCAAGATGATAGAAAAACTGTC
TTTGAAAACATTATCGGCTACTTGTCTACTGGTGGCTATATCATTGTTGCCGTACCTCAT
GAAGACAATGAATTGAGGGCGTATGTAACTAATGATGGTGCTGAGTTTACCGAGGCAAAA
TTCCCATATGATGAAGATATTGGTAAGCAAGACGCATTCACTATTTTAGGATCTGAGGAA
GGATCAATATTTTTACATTTAGCAACAAACTTGGAATCAGGACACGATTTCGGAAACCTT
TTGAAATCCAACTCGAATGGTACTTCTTTTGTCACCTTAGAGCATGCCGTTAATAGAAAC
ACATTCGGTTATGTGGACTTTGAAAAAGTTCAAGGTCTTGAAGGTATTATTATCACCAAC
ATCGTTTCGAATAGCGAAAAGGTTGGCGAGAACAAGGAAGACGAACAATTGAAGACGAAA
ATCACCTTCAATGACGGTTCAGATTGGAACTTTTTGAAGCCTCCAAAGAAGGATTCGGAA
GGGAAAAAGTTTCCCTGTGATTCTGTGTCATTAGATAAATGTTCTCTACATTTGCATGGT
TACACTGAACGTAAGGATATCAGGGATACATATTCCTCTGGCTCCGCGTTAGGAATGATG
TTTGGTGTCGGAAATGTTGGCGATAGGCTTCTACCATATGAGGAGTGTTCCACTTTTCTA
ACCACTGATGGAGGAGAGACGTGGACTGAGGTTAAAAAAGGCCCCCATCAATGGGAGTAC
GGTGATCATGGTGGAGTCTTGGTTTTGGTCCCCGAAAACGCAGAAACTGATTCTATTTCT
TACTCCACCGATTTTGGTAAAACATGGAAAGATTATAAATTCTGTGGCGACAAAGTCCTC
GTAAAGGATATAATCACCGTTCCCAGAGATTCTGCTTTGAGGTTTTTGCTATTTGGAGAA
GCAAAAAACATGGGTAGTGGTTCATTCAGAACGTATACAATTGATTTTAGAAACATCTTC
GAGAGGCAATGTGAGTTTGATATTACCGGTAGAAAAAGGGCAGATTTTAAGTACTCTCCT
CTGGGTTCCAGAACTGGTTGTTTGTTTGGCCATAAAACAGAATTTTTACGTAAAACCGAT
GAAAAATGTTTTATTGGGAATATTCCACTTTCTGAATTTTCGAGGAACGTCAAAAACTGT
CCATGTACAAGACAAGATTTCGAATGTGACTATAATTTTTATAAAGCCAGTGATGGTACT
TGTAAATTAGTCAAAGGCTTAAGTTCGGCGAATGGTGCCGATATCTGCAAAAAGGAACCC
GACTTAATTGAATACTATGATTCCTCCGGCTATCGGAAAATTCCCTTATCAACCTGTAAA
GGTGGGCTGAAATTGGATGCACATTTAGCACCACATCCTTGTCCGGGAAAAGAAAAGGCA
TTCAGGGAAAAATACTCTATAAACACCGGCGCTTATGCGCTGGTTTTTGTTACAATTCTT
CTCGTTATTTTCTTTGTCGCATGGTTTGTATACGATAGAGGTATCAGGAGAAATGGGGGA
TTTTCAAGATTTGAAGAAATCAGATTAGGAGACGATGGGCTGATAGAAAACAATAGGACC
GATAGAGTTGTCAACATCATTGTAAGACTAGGATTATGCATTTCTTTAATCACCAAGTCT
GCGTTTCAACGCGCGAAGGCAGGTACAGCGCAACTTTCTTCAAAATTTAGGGCAAGGTTT
GGCAACAAAAAAGGTGCCACTTACTCTTCGCTGCTTCATGATCAGCTTTCAGATGAACCA
GATGGCTTCCATGAAGATTCCAACGATTTATCCAGTTTCAGAGGTCAAGGTAGTAATTCT
GAAATTGAGCAAGAAGATGTTGACACATCTCAACAAGAGCATACGTTGCGGACAGACCTA
CTTGGTGCTAGCAATATTCCAGACGCTTTGCCAGCGCGTAGTGCTTCTCACGAGTCCGAT
TTAGCAGCTGCACGCAGCGAAGACAAGTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 4650
Start on reference sequence 1
Mutations:
None
Discrepancy:
c4547t,S1516L
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  URA
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pBY011

pBY011 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp): 8121
Parent Vector: Name: YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Empty Vector: None
Properties: Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Bhullar
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3651 4333
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 4915 5124
primer M13 reverse M13 reverse sequencing primer 4702 4722
primer M13 forward M13 forward sequencing primer 7183 7200
promoter Gal1 Gal1-10 yeast promoter 6934 7145
promoter Ura3 promoter URA3 promoter 2349 2378
recombination site attR2 attR recombination site 2, lost or altered in recombined (with insert) form 4749 4764
recombination site attR1 attR recombination site 1, lost or altered in recombined (with insert) form 6439 6455
selectable marker Ura3 URA3 1460 2263
selectable marker CmR chloramphenicol resistance gene LOST in recombined (with insert) form 5566 6222
selectable marker AmpR ampicillin resistance gene 2894 3553
yeast centromere CEN yeast centromeric sequence 255 7396
yeast origin ARS1 ARS1 yeast origin of replication 0 0

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: CAA89519

 

Protein

SMART domain : VPS10 :

Clone

Cloning Information : 261919
HIP Master Clone ID : 177717
Original Clone ID : FLH261919.01X

Organism


Species Specific ID: YJL222W