DNASU Plasmid Repository • 480.965.5697 | Email

ROM2 (Saccharomyces cerevisiae) in pBY011 (yeast expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ROM2
Gene Name:  GDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEP
Sequence             
Original Clone ID: FLH262664.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pBY011               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
ScCD00096983

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4071nts         Open reading frame : 1 to 4071

ATGAGCGAAACCAACGTTGACAGCTTAGGGGACAGAAATGACATATACTCCCAAATATTT
GGTGTAGAAAGGAGGCCAGATTCCTTCGCTACTTTTGATAGTGACTCGCACGGAGACATA
AGTTCTCAGCTGCTGCCTAATAGAATCGAAAATATCCAGAATCTAAATGTTCTGCTTTCT
GAAGACATTGCTAATGACATCATTATTGCCAAGCAGAGGAGACGTTCTGGAGTTGAAGCC
GCCATCGATGATTCTGATATACCAAATAACGAAATGAAGGGGAAAAGCTCAAATTACATT
TTATCGCAGCAGACTAATATTAAGGAAGTTCCAGATACACAAAGTTTGTCTAGTGCTGAT
AATACACCTGTGAGCAGTCCAAAGAAGGCAAGAGATGCCACATCTTCCCATCCCATTGTT
CATGCTAAATCGATGTCCCATATATATTCAACGAGTAATAGTGCTAGTAGACAAGCCAAG
CACTATAATGACCACCCTTTGCCTCCAATGTCTCCAAGAAATGAAGTTTACCAGAAGAAT
AAAAGTACTACAGCTTTTGTGCCTAAGAGGAAACCTTCGCTCCCGCAACTAGCACTTGCT
GGTCTTAAGAAGCAGAGCAGTTTTTCCACCGGCAGTGCATCAACCACTCCTACACAGGCT
AGAAAATCGCCTTTGCAAGGGTTTGGTTTCTTTTCGAGACCAAGTTCCAAAGACTTGCAC
GAACAGCACCAACATCACCAGCATATTCAGCATAATAATATTAACAATCATAATAATAAT
AATACTAATAATAATGGCGCACATTATCAAGTGGGTAGTAGTAACAGTAATTACCCTCAA
CATTCACATTCTATATCATCTAGATCGATGTCGTTGAACTCATCTACTCTAAAAAATATT
GCATCATCTTTTCAATCGAAGACTTCTAACAGCAGGAAAGCTACGCAAAAATATGATATA
ACTTCCAACCCATTCTCAGATCCTCATCATCATCATCATCATCATCACTCTTCAAACAGT
CACAGTAGCCTAAATAATGTCCACGGGTCTGGAAACTCCTCAAGTGTTATGGGAAGCTCG
TCTAATATTGGGTTGGGTCTTAAAACGAGAGTTTCTTCCACCTCATTAGCTTTAAAAAGA
TACACTTCGGTTTCAGGAACTTCTCTATCTTCTCCACGTAGAAGTAGTATGACACCTTTG
TCGGCATCTAGACCTGTTATGAGCGCTTCATCAAAAAAACCTCAGGTATATCCAGCTTTG
TTGTCAAGAGTCGCCACAAAATTTAAAAGTTCGATACAACTCGGGGAACATAAGAAGGAT
GGATTAGTTTACCGTGACGCATTTACAGGGCAGCAGGCAGTCGATGTCATCTGTGCTATC
ATTAGAACTTCTGATAGAAACTTAGCCTTGTTATTTGGTAGATCACTAGATGCACAAAAG
CTGTTCCATGATGTGGTATACGAGCACAGGTTAAGAGATTCACCACATGAAGTTTACGAA
TTTACAGATAACTCAAGATTTACAGGAACAGGTTCCACAAACGCCCATGATCCACTGATG
CTACTCCCAAACTCATCATCTTTTAATTCCGGGAACCACTCTTATCCGAATTCCGGAATG
GTTCCATCAAGTTCAACTTCATCTTTGAATAGTGATCAGGCAACTTTGACAGGGTCGAGG
CTACATATGAGTAGTTCATTGTCCCAGCAAAAGAACCCTGCAGCCATTCATAACGTCAAT
GGTGTGTTTACATTACTGGCGGAATGCTATTCCCCTACGTGCACAAGAGACGCTTTGTGT
TACTCTATTTCGTGTCCAAGACGTCTTGAGCAACAAGCAAGGTTAAATCTGAAACCAAAT
GGCGGCCTGAAACGTAATATATCAATGGCATTGGATGATGATGACGAAGAAAAGCCATCT
TGGACAAGTTCCGTATCTAAAGAGGATTGGGAAAACTTACCAAAAAAGGAAATAAAAAGA
CAAGAGGCTATTTATGAAGTTTATATCACGGAGAAGAACTTTGTAAAATCATTAGAAATT
ACAAGAGACACATTCATGAAGACTTTGGCTGAAACAAATATTATTTCAGCAGATATTAGA
AAAAATTTCATCAAGCATGTTTTTGCACATATAAACGATATCTATTCAGTGAATAGACGT
TTTTTGAAGGCTTTAACTGATAGGCAAAGAAGTTCACCAGTTGTGAGAGGAATTGGGGAC
ATTGTTTTAAGATTCATTCCATTTTTTGAACCATTTGTTTCATATGTCGCTTCAAGACCA
TATGCGAAGTACTTGATTGAAACCCAAAGATCAGTCAATCCCTATTTTGCTAGATTTGAT
GACGATATGATGAGCTCCTCCTTACGTCATGGTATTGATTCGTTTTTATCTCAAGGTGTT
TCAAGACCAGGTCGATATATGCTATTGGTCAAAGAAATCATGAAATCTACTGACCCCGAG
AAGGACAAAAGTGATTATGAAGATTTAAGCAAAGCCATGGATGCATTAAGGGATTTTATG
AAAAGGATTGATCAAGCAAGTGGTGCTGCACAAGATAGACATGATGTAAAATTGTTGAAA
CAAAAGATTCTTTTCAAAAACGAATACGTTAATTTAGGATTAAATGACGAAAGGAGAAAA
ATTAAACATGAAGGTATACTATCACGGAAGGAGTTGTCAAAATCGGACGGAACAGTTGTT
GGTGACATACAATTTTATCTTCTAGATAATATGCTCTTATTTTTAAAGGCCAAAGCAGTT
AATAAATGGCACCAGCATAAGGTATTCCAAAGGCCCATTCCATTGCCATTGTTATTTGCA
TGTCCAGGTGAAGATATGCCTGCGTTGAGGAAATATATTGGAGACCACCCAGATTGTTCA
GGAACTGTAATTCAACCAGAATACAACACGAGTAATCCTAAAAACGCAATAACTTTCTTA
TACTACGGTGCCAAGCAGAGATATCAAGTTACATTATATGCTGCGCAATACGCTGGATTG
CAAACGCTTTTGGAAAAAATAAAGCAAGGACAAGCAGCTATTATAAGCAAAACGGAGATG
TTCAATGTAACGAAAATGAGCGATAGGTTCTTCGATTACACCAACAAAATTAACAGTGTT
ACTTCCTGTGATGGGGGTAGAAAACTTCTGATTGCAACGAACTCTGGATTGTATATGAGC
AACATTAAAAGACAGCAGAATAAAGATCATAGACATAAAAGCTCTGCTTTTTTTTCAACT
CCAATCCAACTAGTACAAAGAAATAATATCACTCAAATTGCTGTATTAGAAGAATTCAAA
TCAATCATACTTTTAATTGATAAAAAATTATACAGTTGTCCGCTGTCATTAATCGAAGCA
GAAGGTAATGGGACATCTTTCTTCAAAAAGCATCACAAGGAACTGATCAATCATGTGAGT
TTCTTCGCAGAAGGAGATTGCAATGGTAAGAGATTAATCGTGACTGCACACAGTTCATCG
CATTCAATTAAGTACTTCGAGCATGAACATCCATTATTGGCCGAAAAAAATGGTAGTGGT
AGCGGTAACAAAAAAAGTCTCAAGAAAAAAATCACCGAGGTTATTTTTGATTCTGAACCT
GTCTCTATTTCATTTCTCAAGGCCAACCTATGCATTGGCTGTAAAAAGGGTTTTCAGATC
GTTAGCATTTCACAGAATGCTCATGAATCCTTGTTAGATCCGGCCGATACATCATTAGAG
TTTGCACTAAGGGATACTTTGAAACCAATGGCAATCTATCGTGTGGGAAATATGTTTTTG
CTGTGTTACACAGAATTTGCCTTCTTTGTCAATAATCAGGGTTGGAGAAAGAAAGAATCG
CATATTATTCATTGGGAGGGAGAGCCCCAAAAATTTGCAATTTGGTACCCGTACATCTTG
GCATTCGACAGCAACTTCATTGAGATCAGAAAGATTGAAACTGGCGAATTAATTCGTTGC
GTCCTTGCTGATAAGATTCGACTTCTACAAACAAGTACTCAAGAAATCCTGTACTGCTAC
GAGGATTATCGCGGGTATGATACAGTTGCGTCGTTAGATTTCTGGGGTTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 4071
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  URA
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pBY011

pBY011 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp): 8121
Parent Vector: Name: YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Empty Vector: None
Properties: Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Bhullar
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3651 4333
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 4915 5124
primer M13 reverse M13 reverse sequencing primer 4702 4722
primer M13 forward M13 forward sequencing primer 7183 7200
promoter Gal1 Gal1-10 yeast promoter 6934 7145
promoter Ura3 promoter URA3 promoter 2349 2378
recombination site attR2 attR recombination site 2, lost or altered in recombined (with insert) form 4749 4764
recombination site attR1 attR recombination site 1, lost or altered in recombined (with insert) form 6439 6455
selectable marker Ura3 URA3 1460 2263
selectable marker CmR chloramphenicol resistance gene LOST in recombined (with insert) form 5566 6222
selectable marker AmpR ampicillin resistance gene 2894 3553
yeast centromere CEN yeast centromeric sequence 255 7396
yeast origin ARS1 ARS1 yeast origin of replication 0 0

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: AAB67564

 

Clone

Cloning Information : 262664
HIP Master Clone ID : 124989
Original Clone ID : FLH262664.01X

Organism


Species Specific ID: YLR371W