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CCNB2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CCNB2
Gene Name:  cyclin B2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH009094.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000998

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1197nts         Open reading frame : 1 to 1197
Reference Sequence Alignment

ATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGGAGAATATTGACACAGGAGTT
AATTCTAAAGTTAAGAGTCATGTGACTATTAGGCGAACTGTTTTAGAAGAAATTGGAAAT
AGAGTTACAACCAGAGCAGCACAAGTAGCTAAGAAAGCTCAGAACACCAAAGTTCCAGTT
CAACCCACCAAAACAACAAATGTCAACAAACAACTGAAACCTACTGCTTCTGTCAAACCA
GTACAGATGGAAAAGTTGGCTCCAAAGGGTCCTTCTCCCACACCTGAGGATGTCTCCATG
AAGGAAGAGAATCTCTGCCAAGCTTTTTCTGATGCCTTGCTCTGCAAAATCGAGGACATT
GATAACGAAGATTGGGAGAACCCTCAGCTCTGCAGTGACTACGTTAAGGATATCTATCAG
TATCTCAGGCAGCTGGAGGTTTTGCAGTCCATAAACCCACATTTCTTAGATGGAAGAGAT
ATAAATGGACGCATGCGTGCCATCCTAGTGGATTGGCTGGTACAAGTCCACTCCAAGTTT
AGGCTTCTGCAGGAGACTCTGTACATGTGCGTTGGCATTATGGATCGATTTTTACAGGTT
CAGCCAGTTTCCCGGAAGAAGCTTCAGTTAGTTGGGATTACTGCTCTGCTCTTGGCTTCC
AAGTATGAGGAGATGTTTTCTCCAAATATTGAAGACTTTGTTTACATCACAGACAATGCT
TATACCAGTTCCCAAATCCGAGAAATGGAAACTCTAATTTTGAAAGAATTGAAATTTGAG
TTGGGTCGACCCTTGCCACTACACTTCTTAAGGCGAGCATCAAAAGCCGGGGAGGTTGAT
GTTGAACAGCACACTTTAGCCAAGTATTTGATGGAGCTGACTCTCATCGACTATGATATG
GTGCATTATCATCCTTCTAAGGTAGCAGCAGCTGCTTCCTGCTTGTCTCAGAAGGTTCTA
GGACAAGGAAAATGGAACTTAAAGCAGCAGTATTACACAGGATACACAGAGAATGAAGTA
TTGGAAGTCATGCAGCACATGGCCAAGAATGTGGTGAAAGTAAATGAAAACTTAACTAAA
TTCATCGCCATCAAGAATAAGTATGCAAGCAGCAAACTCCTGAAGATCAGCATGATCCCT
CAGCTGAACTCAAAAGCCGTCAAAGACCTTGCCTCCCCACTGATAGGAAGGTCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1345
Start on reference sequence 149
Mutations:
None
Discrepancy:
a627g
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCNB2
Symbol Nomenclature : CCNB2
SYNONYM : HsT17299
Designation : G2/mitotic-specific cyclin-B2
Full Nomenclature : cyclin B2
GENEID : 9133
GI : 61367951
GenBank Accession : AY891142
HGNC : 1580
MIM : 602755
Vega : OTTHUMG00000132715
Target GenBank: NM_004701

 

Reference Sequence Alignment

  

HsCD00000998      ATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGGAGAATATTGACACAGGAGTT
NM_004701.3       ATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGGAGAATATTGACACAGGAGTT
                  ************************************************************

HsCD00000998      AATTCTAAAGTTAAGAGTCATGTGACTATTAGGCGAACTGTTTTAGAAGAAATTGGAAAT
NM_004701.3       AATTCTAAAGTTAAGAGTCATGTGACTATTAGGCGAACTGTTTTAGAAGAAATTGGAAAT
                  ************************************************************

HsCD00000998      AGAGTTACAACCAGAGCAGCACAAGTAGCTAAGAAAGCTCAGAACACCAAAGTTCCAGTT
NM_004701.3       AGAGTTACAACCAGAGCAGCACAAGTAGCTAAGAAAGCTCAGAACACCAAAGTTCCAGTT
                  ************************************************************

HsCD00000998      CAACCCACCAAAACAACAAATGTCAACAAACAACTGAAACCTACTGCTTCTGTCAAACCA
NM_004701.3       CAACCCACCAAAACAACAAATGTCAACAAACAACTGAAACCTACTGCTTCTGTCAAACCA
                  ************************************************************

HsCD00000998      GTACAGATGGAAAAGTTGGCTCCAAAGGGTCCTTCTCCCACACCTGAGGATGTCTCCATG
NM_004701.3       GTACAGATGGAAAAGTTGGCTCCAAAGGGTCCTTCTCCCACACCTGAGGATGTCTCCATG
                  ************************************************************

HsCD00000998      AAGGAAGAGAATCTCTGCCAAGCTTTTTCTGATGCCTTGCTCTGCAAAATCGAGGACATT
NM_004701.3       AAGGAAGAGAATCTCTGCCAAGCTTTTTCTGATGCCTTGCTCTGCAAAATCGAGGACATT
                  ************************************************************

HsCD00000998      GATAACGAAGATTGGGAGAACCCTCAGCTCTGCAGTGACTACGTTAAGGATATCTATCAG
NM_004701.3       GATAACGAAGATTGGGAGAACCCTCAGCTCTGCAGTGACTACGTTAAGGATATCTATCAG
                  ************************************************************

HsCD00000998      TATCTCAGGCAGCTGGAGGTTTTGCAGTCCATAAACCCACATTTCTTAGATGGAAGAGAT
NM_004701.3       TATCTCAGGCAGCTGGAGGTTTTGCAGTCCATAAACCCACATTTCTTAGATGGAAGAGAT
                  ************************************************************

HsCD00000998      ATAAATGGACGCATGCGTGCCATCCTAGTGGATTGGCTGGTACAAGTCCACTCCAAGTTT
NM_004701.3       ATAAATGGACGCATGCGTGCCATCCTAGTGGATTGGCTGGTACAAGTCCACTCCAAGTTT
                  ************************************************************

HsCD00000998      AGGCTTCTGCAGGAGACTCTGTACATGTGCGTTGGCATTATGGATCGATTTTTACAGGTT
NM_004701.3       AGGCTTCTGCAGGAGACTCTGTACATGTGCGTTGGCATTATGGATCGATTTTTACAGGTT
                  ************************************************************

HsCD00000998      CAGCCAGTTTCCCGGAAGAAGCTTCAGTTAGTTGGGATTACTGCTCTGCTCTTGGCTTCC
NM_004701.3       CAGCCAGTTTCCCGGAAGAAGCTTCAATTAGTTGGGATTACTGCTCTGCTCTTGGCTTCC
                  **************************.*********************************

HsCD00000998      AAGTATGAGGAGATGTTTTCTCCAAATATTGAAGACTTTGTTTACATCACAGACAATGCT
NM_004701.3       AAGTATGAGGAGATGTTTTCTCCAAATATTGAAGACTTTGTTTACATCACAGACAATGCT
                  ************************************************************

HsCD00000998      TATACCAGTTCCCAAATCCGAGAAATGGAAACTCTAATTTTGAAAGAATTGAAATTTGAG
NM_004701.3       TATACCAGTTCCCAAATCCGAGAAATGGAAACTCTAATTTTGAAAGAATTGAAATTTGAG
                  ************************************************************

HsCD00000998      TTGGGTCGACCCTTGCCACTACACTTCTTAAGGCGAGCATCAAAAGCCGGGGAGGTTGAT
NM_004701.3       TTGGGTCGACCCTTGCCACTACACTTCTTAAGGCGAGCATCAAAAGCCGGGGAGGTTGAT
                  ************************************************************

HsCD00000998      GTTGAACAGCACACTTTAGCCAAGTATTTGATGGAGCTGACTCTCATCGACTATGATATG
NM_004701.3       GTTGAACAGCACACTTTAGCCAAGTATTTGATGGAGCTGACTCTCATCGACTATGATATG
                  ************************************************************

HsCD00000998      GTGCATTATCATCCTTCTAAGGTAGCAGCAGCTGCTTCCTGCTTGTCTCAGAAGGTTCTA
NM_004701.3       GTGCATTATCATCCTTCTAAGGTAGCAGCAGCTGCTTCCTGCTTGTCTCAGAAGGTTCTA
                  ************************************************************

HsCD00000998      GGACAAGGAAAATGGAACTTAAAGCAGCAGTATTACACAGGATACACAGAGAATGAAGTA
NM_004701.3       GGACAAGGAAAATGGAACTTAAAGCAGCAGTATTACACAGGATACACAGAGAATGAAGTA
                  ************************************************************

HsCD00000998      TTGGAAGTCATGCAGCACATGGCCAAGAATGTGGTGAAAGTAAATGAAAACTTAACTAAA
NM_004701.3       TTGGAAGTCATGCAGCACATGGCCAAGAATGTGGTGAAAGTAAATGAAAACTTAACTAAA
                  ************************************************************

HsCD00000998      TTCATCGCCATCAAGAATAAGTATGCAAGCAGCAAACTCCTGAAGATCAGCATGATCCCT
NM_004701.3       TTCATCGCCATCAAGAATAAGTATGCAAGCAGCAAACTCCTGAAGATCAGCATGATCCCT
                  ************************************************************

HsCD00000998      CAGCTGAACTCAAAAGCCGTCAAAGACCTTGCCTCCCCACTGATAGGAAGGTCCTTG
NM_004701.3       CAGCTGAACTCAAAAGCCGTCAAAGACCTTGCCTCCCCACTGATAGGAAGGTCCTAG
                  *******************************************************:*

Pathways

NCI : FOXM1 transcription factor network
NCI : Validated transcriptional targets of deltaNp63 isoforms
Reactome : Activation of NIMA Kinases NEK9, NEK6, NEK7
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Condensation of Prometaphase Chromosomes
Reactome : Cyclin A/B1 associated events during G2/M transition
Reactome : Cyclin B2 mediated events
Reactome : G2/M Checkpoints
Reactome : G2/M DNA replication checkpoint
Reactome : G2/M Transition
Reactome : Golgi Cisternae Pericentriolar Stack Reorganization
Reactome : M Phase
Reactome : Mitotic G2-G2/M phases
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Prometaphase
Reactome : Mitotic Prophase
Reactome : Nuclear Envelope Breakdown
Reactome : Nuclear Pore Complex (NPC) Disassembly
Reactome : Polo-like kinase mediated events
Reactome : Regulation of PLK1 Activity at G2/M Transition
Reactome : Resolution of Sister Chromatid Cohesion
WikiPathway : Cell cycle
WikiPathway : DNA damage response
WikiPathway : TGF beta Signaling Pathway
WikiPathway : miRNA regulation of DNA Damage Response

Clone

Cloning Information : 9094
HIP Master Clone ID : 11662
Original Clone ID : FLH009094.01L

Organism


TAX_ID : 9606
Species Specific ID: 9133

Genome

Chromosome : 15
Map Location : 15q22.2
Ensembl : ENSG00000157456

Reagents

Labome : CCNB2-antibody