Detailed Vector Information: pDNR-Dual

Vector Name:
pDNR-Dual
Synonyms:
''
Sequencing Primer:
Forward:  M13F
Reverse:  Unknown
Description:
Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments:
The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp):
4938
Parent Vector:
None
Properties:
Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
MCS
MCS
restriction enzyme-type multiple cloning site
45
129
bacterial origin
ColE1
ColE1 (pUC-type) origin of replication
4111
4574
negative selection marker
SacB
SacB negative selection marker gene (sucrose sensitivity)
1057
2972
primer site
M13 primer
M13 primer sequencing site
4839
4855
primer site
T7 primer
T7 RNA Pol primer sequencing site
4865
4883
recombination site
loxP site
loxP recombination site
9
42
recombination site
loxP site
loxP recombination site
1015
1048
selectable marker
CmR
chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter)
986
327
selectable marker
AmpR
ampicillin resistance gene
3103
3963
splice donor site
SD site
splice donor site
132
136
tag
HN
6xHN epitope tag
141
176