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Detailed Vector Information: pBY011_GFP-NH3_CN


Vector Name: pBY011_GFP-NH3_CN
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Synonyms: ''


Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector, adds N-terminal fluorescent (GFP) tag; amp resistance; recombinational cloning.
Comments: This vector appears to be derived from pBY011-D123
Size (bp): 7920
Empty Vector: None
Parent Vector: None
Properties: Gateway, acceptor (destination), fluorescent marker, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name:
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available


Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori origin of replication 5118 5800
bacterial origin ColE1 ori ColE1 origin of replication 0 0
negative selection marker ccdB ccdB death cassette ONLY in Non-Recombined (NO INSERT) form 6381 6590
promoter Gal 1-10 Gal 1-10 promoter (yeast) 0 0
recombination site attR1 att recombination site 7901 7917
recombination site attB1 att recombination (half?) site 0 0
recombination site attB2 att recombination (half?) site 0 0
recombination site attR2 att recombination site 6215 6230
selectable marker CmR chlorampenicol resistance gene ONLY In Non-Recombined (NO INSERT) form 7028 7684
selectable marker URA3 URA3 nutritional marker 0 0
selectable marker AmpR ampicillin resistance gene 4361 5020
tag GFP Green fluorescent protein coding region 0 0
yeast ARS ARS1 ARS1 replication sequence (yeast) 0 0
yeast centromere CEN4 CEN4 yeast centromeric sequence 0 0