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Detailed Vector Information: pENTR221

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Vector Name: pENTR221
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Synonyms: pDONR221, pENTR221-XnCDSYn

pENTR221

Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.
Size (bp): 2574
Empty Vector: None
Parent Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 1905 4
primer site M13 F M13 forward priming site 846 865
primer site M13 Rev M13 reverse primer site 846 865
recombination site rrnBT2 rrnB T2 transcription termination sequence 275 302
recombination site rrnBT1 rrnB T1 transcription termination sequence 434 477
recombination site attL1 attL1 recombination site 575 675
recombination site attL2 attL2 recombination site 805 706
selectable marker KanR kanamycin resistance gene 975 1784